Mechanisms of Chinese medical formula Fangji Huangqi Decoction as an effective treatment of nephrotic syndrome based on systems pharmacology

Zhang, Wang-Ning; Yang, Liu; He, Shengsheng; Li, Aiping; Qin, Xuemei

doi:10.1016/j.chmed.2019.05.005

Cited by 2 publications

(2 citation statements)

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“…In recent years, TCMs are increasingly becoming the promising therapeutic drugs for kidney diseases for their low side effects and commendable pharmacological activities [ 33 ]. FHD is a classical TCMs firstly recorded in Synopsis of the Golden Chamber and widely used in the treatment of edema and dysuria [ 4 ]. In this study, based on network pharmacology and functional enrichment analyses, 9 hub targets (CCL2, IL-10, PTGS2, TNF, MAPK1, IL-6, CXCL8, TP53, and VEGFA) and PI3K-Akt pathway were predicted as the potential target mechanisms for FHD treating NS.…”

Section: Discussionmentioning

confidence: 99%

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Network Pharmacology and In Vivo Experimental Validation to Uncover the Renoprotective Mechanisms of Fangji Huangqi Decoction on Nephrotic Syndrome

Yin

Mao

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Background. Fangji Huangqi decoction (FHD) is a traditional Chinese medicine formula that has the potential efficacy for nephrotic syndrome (NS) treatment. This study aims to explore the effects and underlying mechanisms of FHD against NS via network pharmacology and in vivo experiments. Methods. The bioactive compounds and targets of FHD were retrieved from the TCMSP database. NS-related targets were collected from GeneCards and DisGeNET databases. The compound-target and protein-protein interaction networks were constructed by Cytoscape 3.8 and BisoGenet, respectively. GO and KEGG analyses were performed by the DAVID online tool. The interactions between active compounds and hub genes were revealed by molecular docking. An NS rat model was established to validate the renoprotective effects and molecular mechanisms of FHD against NS in vivo. Results. A total of 32 hub genes were predicted to play essential roles in FHD treating NS. Eight main bioactive compounds of FHD had the good affinity with 9 hub targets (CCL2, IL-10, PTGS2, TNF, MAPK1, IL-6, CXCL8, TP53, and VEGFA). The therapeutic effect of FHD on NS was closely involved in the regulation of inflammation and PI3K-Akt pathway. In vivo experiments confirmed the renoprotective effect of FHD on NS, evidenced by reducing the levels of proteinuria, serum creatinine, blood urea nitrogen, and inflammatory factors in NS rats. The PI3K activator 740Y-P weakened the effects of FHD against NS. Furthermore, FHD downregulated the levels of PTGS2, MAPK1, IL-6, and p-Akt in NS rats. Conclusions. FHD alleviates kidney injury and inflammation in NS by targeting PTGS2, MAPK1, IL-6, and PI3K-Akt pathway.

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Section: Discussionmentioning

confidence: 99%

“…Traditional Chinese medicines (TCMs) have attracted great attention for NS treatment, due to their favorable properties of low side effects, recurring rate, and cost [ 4 ]. Importantly, TCMs exert therapeutic effects by coordinating multicompounds and multitargets.…”

Section: Introductionmentioning

confidence: 99%