Mechanisms of Carbapenem Resistance in Multidrug-Resistant Clinical Isolates of<i>Pseudomonas aeruginosa</i>from a Croatian Hospital

Bubonja‐Šonje, Marina; Matovina, Mihaela; Škrobonja, Ivana; Bedenić, Branka; Abram, Maja

doi:10.1089/mdr.2014.0172

Cited by 36 publications

(24 citation statements)

References 42 publications

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“…Both species showed resistance to cephalosporins. Previous studies from Canada, Croatia, and Latin America found P. aeruginosa isolates resistant to carbapenems, aminoglycosides, and ciprofloxacin but not to piperacillin [14, 15]. Citrobacter isolates showed resistance to eight antibiotics whereas they were moderately susceptible to other antibiotics.…”

Section: Resultsmentioning

confidence: 99%

Antibiotic Susceptibility Patterns of Bacterial Isolates from Pus Samples in a Tertiary Care Hospital of Punjab, India

Trojan

Razdan

Singh

2016

International Journal of Microbiology

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We determined the prevalence and antibiotic susceptibilities patterns of bacterial isolates from pus samples collected from patients in a tertiary care hospital of Punjab, India. E. coli was the most prevalent pathogen (51.2%) followed by Staphylococcus aureus (21%), Klebsiella pneumoniae (11.6%), Pseudomonas aeruginosa (5.8%), Citrobacter spp. (3.5%), Acinetobacter baumannii (2.3%), Proteus mirabilis (2.3%), and Streptococcus spp. (2.3%). E. coli, K. pneumoniae, A. baumannii, and Citrobacter isolates were resistant to multiple antibiotics including higher generation cephalosporins. S. aureus and Streptococcus isolates were sensitive to cloxacillin and vancomycin. However, P. aeruginosa, P. mirabilis, and Streptococcus isolates were found to be less resistant to the spectrum of antibiotics tested. Overall, our findings indicate the prevalence of resistance to different classes of antibiotics in bacterial isolates from pus infections and hence highlight the need for effective surveillance, regulator reporting, and antibiogram-guided antibiotic prescription.

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Section: Resultsmentioning

confidence: 99%

Antibiotic Susceptibility Patterns of Bacterial Isolates from Pus Samples in a Tertiary Care Hospital of Punjab, India

Trojan

Razdan

Singh

2016

International Journal of Microbiology

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“…Over the past decades, the frequency of antimicrobial resistance has increased globally. MDR bacteria are now recognized as a major cause of nosocomial infections, representing a serious safety concern for both health care practitioners and the patients [9,14]. The risk of MDR bacterial infection has been related to a number of factors, including long-term antimicrobial therapy, cross-transmission, length of hospital stay, and invasive procedures [15,16].…”

Section: Discussionmentioning

confidence: 99%

“…Common bacteria that show MDR/PDR are methicillin-resistant Staphylococcus aureus (MRSA), extended-spectrum beta-lactamase (ESBL)-producing bacteria, carbapenem-resistant Enterobacteriaceae (CRE), carbapenem-resistant Acinetobacter baumannii (A. baumannii, CRAB), MDR/PDR Pseudomonas aeruginosa (P. aeruginosa, MDR/PDRPA), and MDR Mycobacterium tuberculosis [6][7][8]. A high prevalence of nosocomial infections attributable to MDR strains has been reported in countries worldwide [8][9][10][11][12]. In 2014, the average MRSA detection rate across 17 Chinese hospitals was 44.6% (29.1% to 74.2%), and the detection rates of ESBL-producing Escherichia coli (E. coli, ESBLECO) and ESBL-producing Klebsiella pneumoniae (ESBLKPN) were 55.8% and 29.9%, respectively [10].…”

Section: Introductionmentioning

confidence: 99%

Analysis of multidrug-resistant bacteria in 3223 patients with hospital-acquired infections (HAI) from a tertiary general hospital in China

Wang¹,

H²,

Zhao

et al. 2019

Bosn J of Basic Med Sci

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The frequency of antimicrobial resistance has increased globally due to misuse and overuse of antibiotics, and multi-drug resistant (MDR) bacteria are now recognized as a major cause of hospital-acquired infections (HAI). Our aim was to investigate the prevalence, distribution, and antimicrobial susceptibility rates of MDR bacteria in patients with HAI from a tertiary hospital in China. We retrospectively evaluated all patients with a confirmed diagnosis of bacterial infection at a tertiary general hospital in Jining, for the period between January 2012 and December 2014. The following clinical and demographic data were collected: age, sex, specimens, treatment, microbiology results, and antibiotic resistance patterns of isolates. Bacterial identification and susceptibility testing were performed using VITEK 2 COMPACT system. We screened a total of 15,588 patients, out of which 7579 (48.6%) had an HAI. MDR showed 3223 out of 7579 isolates (42.5%). The most frequently isolated MDR bacteria in patients with HAI were extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli (n = 1216/3223, 37.7%), MDR Pseudomonas aeruginosa (n = 627/3223, 19.5%) and MDR Acinetobacter baumannii (n = 588/3223, 18.2%). MDR-HAI were more common in males (2074/3223, 64.4%) and in elderly patients (≥60 years; 1196/3223, 37.1%). Sputum was the main source of MDR isolates (2056/3223, 63.8%). Patients with MDR-HAI were predominantly distributed in different types of intensive care units. MDR strains in our study showed resistance to most current antibiotics. Overall, patients with HAI infections attributed to MDR bacteria were widely distributed in our hospital. Enhanced surveillance of MDR bacteria is critical for guiding the rational use of antibiotics and reducing the incidence of HAI.

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“…␤-lactams (4,5). The chromosomally encoded broad-spectrum AmpC ␤-lactamase contributes to the natural resistance of P. aeruginosa to many ␤-lactam antibiotics together with MexAB-OprM (6).…”

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confidence: 99%

Antimicrobial Effects of β-Lactams on Imipenem-Resistant Ceftazidime-Susceptible Pseudomonas aeruginosa

Choi

Lee

et al. 2017

Antimicrob Agents Chemother

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We studied the resistance mechanism and antimicrobial effects of ␤-lactams on imipenem-resistant Pseudomonas aeruginosa isolates that were susceptible to ceftazidime as detected by time-kill curve methods. Among 215 P. aeruginosa isolates from hospitalized patients in eight hospitals in the Republic of Korea, 18 isolates (23.4% of 77 imipenem-resistant isolates) were imipenem resistant and ceftazidime susceptible. Multilocus sequence typing revealed diverse genotypes, which indicated independent emergence. These 18 isolates were negative for carbapenemase genes. All 18 imipenem-resistant ceftazidime-susceptible isolates showed decreased mRNA expression of oprD, and overexpression of mexB was observed in 13 isolates. In contrast, overexpression of ampC, mexD, mexF, or mexY was rarely found. Time-kill curve methods were applied to three selected imipenem-resistant ceftazidime-susceptible isolates at a standard inoculum (5 ϫ 10 5 CFU/ml) or at a high inoculum (5 ϫ 10 7 CFU/ml) to evaluate the antimicrobial effects of ␤-lactams. Inoculum effects were detected for all three ␤-lactam antibiotics, ceftazidime, cefepime, and piperacillin-tazobactam, against all three isolates. The antibiotics had significant killing effects in the standard inoculum, but no effects in the high inoculum were observed. Our results suggest that ␤-lactam antibiotics should be used with caution in patients with imipenem-resistant ceftazidime-susceptible P. aeruginosa infection, especially in high-inoculum infections such as endocarditis and osteomyelitis.

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Mechanisms of Carbapenem Resistance in Multidrug-Resistant Clinical Isolates ofPseudomonas aeruginosafrom a Croatian Hospital

Cited by 36 publications

References 42 publications

Antibiotic Susceptibility Patterns of Bacterial Isolates from Pus Samples in a Tertiary Care Hospital of Punjab, India

Antibiotic Susceptibility Patterns of Bacterial Isolates from Pus Samples in a Tertiary Care Hospital of Punjab, India

Analysis of multidrug-resistant bacteria in 3223 patients with hospital-acquired infections (HAI) from a tertiary general hospital in China

Antimicrobial Effects of β-Lactams on Imipenem-Resistant Ceftazidime-Susceptible Pseudomonas aeruginosa

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