Mechanisms of bone repair and regeneration

Deschaseaux, Frédéric; Sensebé, Luc; Heymann, Dominique

doi:10.1016/j.molmed.2009.07.002

Cited by 267 publications

(247 citation statements)

References 99 publications

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“…41,42 Growth factors that stimulate cell growth, differentiation, and vascularization have become a common applicable tool to induce bone formation. Several ceramic, polymer, composite systems have been adapted to maximize their therapeutic potential at the target site.…”

Section: Discussionmentioning

confidence: 99%

Effect of Local Sustainable Release of BMP2-VEGF from Nano-Cellulose Loaded in Sponge Biphasic Calcium Phosphate on Bone Regeneration

Sukul

Linh

Min

et al. 2015

Tissue Engineering Part A

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Bone regeneration is a coordinated process mainly regulated by multiple growth factors. Vascular endothelial growth factor (VEGF) stimulates angiogenesis and bone morphogenetic proteins (BMPs) induce osteogenesis during bone healing process. The aim of this study was to investigate how these growth factors released locally and sustainably from nano-cellulose (NC) simultaneously effect bone formation. A biphasic calcium phosphate (BCP)-NC-BMP2-VEGF (BNBV) scaffold was fabricated for this purpose. The sponge BCP scaffold was prepared by replica method and then loaded with 0.5% NC containing BMP2-VEGF. Growth factors were released from NC in a sustainable manner from 1 to 30 days. BNBV scaffolds showed higher cell attachment and proliferation behavior than the other scaffolds loaded with single growth factors. Bare BCP scaffolds and BNBV scaffolds seeded with rat bone marrow mesenchymal stem cells were implanted ectopically and orthotopically in nude mice for 4 weeks. No typical bone formation was exhibited in BNBV scaffolds in ectopic sites. BMP2 and VEGF showed positive effects on new bone formation in BNBV scaffolds, with and without seeded stem cells, in the orthotopic defects. This study demonstrated that the BNBV scaffold could be beneficial for improved bone regeneration. Stem cell incorporation into this scaffold could further enhance the bone healing process.

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Section: Discussionmentioning

confidence: 99%

Effect of Local Sustainable Release of BMP2-VEGF from Nano-Cellulose Loaded in Sponge Biphasic Calcium Phosphate on Bone Regeneration

Sukul

Linh

Min

et al. 2015

Tissue Engineering Part A

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“…Images of the most central three sections of the defects were obtained and stored as digital files for histomorphometric analysis. The following parameters were measured: (1) distance between the margins of the original surgical defect (defect length: DL); (2) length of the internal bone bridging formation (defect closure: DC); (3) total defect area (TA), determined by firstly identifying the external and internal surfaces of the original calvarium at the right and left margins of the surgical defect, and then connecting them with lines drawn along their respective curvatures; (4) region stained with hematoxylin/eosin in the TA, which was color extracted and defined as the newly formed bone area (NBA); and (5) vertical augmentation height, as measured at four points, two at 1 mm from the center of the defect and two at 1 mm internally from the defect margins, with each pair of measurements averaged to yield the mean central bone height (CBH) and marginal bone height (MBH). These heights were composed of calcified osseous tissue, grafted biomaterial, and/or the soft tissue interface 24) .…”

Section: Histological and Histomorphometric Analysesmentioning

confidence: 99%

“…In vitro and in vivo studies have revealed that several factors, including bone morphogenetic proteins (BMPs), are involved in bone development, repair and regeneration 1) . In particular, BMP-2, which possesses potent osteoinductive activity, has been extensively studied 2) .…”

Section: Introductionmentioning

confidence: 99%

Bone healing capabilities of recombinant human bone morphogenetic protein-9 (rhBMP-9) with a chitosan or collagen carrier in rat calvarial defects

et al. 2016

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The aim of this study was to examine the effects of recombinant human BMP-9 (rhBMP-9) with chitosan sponge (ChiS) or absorbable collagen sponge (ACS) on bone formation in rat calvarial defects. The defects were treated by one of the following implantations: ChiS, rhBMP-9/ChiS, ACS, rhBMP-9/ACS and no implantation. The animals were euthanized at 8 weeks for histological evaluation. The percentage of defect closure (DC) in the rhBMP-9/ACS group was significantly greater than that in the ACS group. The rhBMP-9/ ACS group demonstrated the highest level of DC among all the groups. The newly formed bone area (NBA) and NBA/total area in the ChiS-implanted groups and in the rhBMP-9/ACS group were significantly greater compared with those in the ACS group. It can be concluded that rhBMP-9/ACS has a potential to induce bone formation in rat calvarial defects. Further studies are required to elucidate the mechanism of bone formation induced by rhBMP-9.

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“…Age-related changes in bone β-catenin protein and sclerostin levels in PAM and NPAM Given that the activation of canonical Wnt pathway is a major mechanism to increase bone accrual (Glass et al 2005;Khosla et al 2008;Deschaseaux et al 2009), we measured the protein levels of β-catenin, the main effector of this pathway, in the tibia of both types of aging mice. We found that these levels were significantly diminished in adult PAM, and further decreased to almost undetectable values in both types of aged mice (Fig.…”

Section: Resultsmentioning

confidence: 99%

Characterization of skeletal alterations in a model of prematurely aging mice

Portal‐Núñez

Manassra

Lozano

et al. 2012

AGE

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An age-related bone loss occurs, apparently associated with the concomitant increase in an oxidative stress situation. However, the underlying mechanisms of age-related osteopenia are ill defined since these studies are time consuming and require the use of many animals (mainly rodents). Here, we aimed to characterize for the first time the bone status of prematurely aging mice (PAM), which exhibit an increased oxidative stress. Tibiae from adult (6 months) PAM show an increase in bone mineral density (BMD) and bone mineral content (assessed by bone densitometry) versus those in their normal counterparts (non-prematurely aging mice, NPAM) and similarly decreased in both kinds of mouse with age. However, at this bone site, trabecular BMD (determined by μ-computerized tomography) was similar in both adult PAM and old (18 months) NPAM. Femurs from these groups of mice present an increase in oxidative stress, inflammation, osteoclastogenic, and adipogenic markers, but a decrease in the gene expression of osteoblastic differentiation markers and of the Wnt/β-catenin pathway. Our findings show that adult PAM recapitulate various age-related bone features, and thus are a suitable model for premature bone senescence studies.

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Mechanisms of bone repair and regeneration

Cited by 267 publications

References 99 publications

Effect of Local Sustainable Release of BMP2-VEGF from Nano-Cellulose Loaded in Sponge Biphasic Calcium Phosphate on Bone Regeneration

Effect of Local Sustainable Release of BMP2-VEGF from Nano-Cellulose Loaded in Sponge Biphasic Calcium Phosphate on Bone Regeneration

Bone healing capabilities of recombinant human bone morphogenetic protein-9 (rhBMP-9) with a chitosan or collagen carrier in rat calvarial defects

Characterization of skeletal alterations in a model of prematurely aging mice

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