Mechanisms of biofilm formation in Staphylococcus epidermidis and Staphylococcus aureus: functional molecules, regulatory circuits, and adaptive responses

Mack, Dietrich; Becker, Petra; Chatterjee, Indranil; Dobinsky, Sabine; Knobloch, J; Peters, Georg; Rohde, Holger; Herrmann, Mathias

doi:10.1016/j.ijmm.2004.06.015

Cited by 187 publications

(124 citation statements)

References 92 publications

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“…These results indicated that several properties of bacterial cells (the surface properties, gene expression, etc) were also changed after biosurfactant treatment. The situation in natural systems is obviously more complex and requires the consideration of many additional parameters (15,39).…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus acidophilus-derived biosurfactant effect on gtfB and gtfC expression level in Streptococcus mutans biofilm cells

Tahmourespour¹,

Salehi²,

Kermanshahi³

2011

Braz. J. Microbiol.

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Streptococcus mutans (S. mutans), harboring biofilm formation, considered as a main aetiological factor of dental caries. Gtf genes play an important role in S. mutans biofilm formation. The purpose of this study was to investigate the effect of Lactobacillus acidophilus-derived biosurfactant on S. mutans biofilm formation and gtfB/C expression level (S. mutans standard strain ATCC35668 and isolated S. mutans strain (22) Several properties of S. mutans cells (the surface properties, biofilm formation, adhesion ability and gene expression) were changed after L. acidophilus-derived biosurfactant treatment. It is also concluded that biosurfacant treatment can provide an optional way to control biofilm development. On the basis of our findings, we can suggest that the prepared biosurfactant may interfere with adhesion processes of S. mutans to teeth surfaces, provided additional evaluation produce satisfactory results.

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Section: Discussionmentioning

confidence: 99%

Lactobacillus acidophilus-derived biosurfactant effect on gtfB and gtfC expression level in Streptococcus mutans biofilm cells

Tahmourespour¹,

Salehi²,

Kermanshahi³

2011

Braz. J. Microbiol.

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“…[21], due to the presence of adherent, multilayered biofilms on the surfaces of implanted biomedical devices which are less susceptible to antibiotics compared to their planktonic counterparts [22]. P. aeruginosa has been found to infect surgical procedures such as Total Knee Arthroplasty (TKA) [23], and is reported to be the cause of osteomyelitis in pelvic bones [24].…”

Section: Introductionmentioning

confidence: 99%

Comparison of antibacterial properties of commercial bone cements and fillers with a zinc-based glass polyalkenoate cement

2010

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“…Studies of Staphylococcus species, which normally inhabit the human oral cavity, suggest that the key purine metabolic gene, purL in the purine metabolic pathway plays an important role in the oral biofilm formation known as dental plaque. [35][36][37][38][39][40][41][42] Further evidence is also available suggesting that purine is usually combined with sugars to participate in the synthesis of the polysaccharide components of cell walls and capsules causing biofilm formation. 39 Therefore, it is reasonable to predict that biofilm formation is highly influenced by purine metabolism.…”

Section: Discussionmentioning

confidence: 99%

Characterization of inosine monophosphate dehydrogenase from Staphylococcus aureus ATCC12600 and its involvement in biofilm formation

Yeswanth¹,

Kumar²,

Swarupa³

et al. 2013

JCSR

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Background: In Staphylococcus aureus purine metabolism plays a crucial role in the formation of biofilm which is a key pathogenic factor. The present study is aimed in the characterization of inosine monophosphate dehydrogenase (IMPDH) from Staphylococcus aureus ATCC 12600.Methods: IMPDH gene was amplified using primers designed from IMPDH gene sequence of S. aureus reported in the database. Then polymerase chain reaction (PCR) product was cloned in the Sma I site of M13mp18 and expressed in Escherichia coli JM109. The recombinant IMPDH (rIMPDH) was overexpressed with 1 mM isopropyl beta-D-1-thiogalactopyranoside (IPTG); Michaelis constant (Km), maximum enzyme velocity (Vmax) and catalytic constant (Kcat) of expressed IMPDH were determined. Results:The enzyme kinetics of IMPDH grown under aerobic conditions showed a Km of 43.71±1.56 µM, Vmax of 0.247±0.84/µM/mg/min and Kcat of 2.74±0.015/min while in anaerobic conditions the kinetics showed Km of 42.81±3.154/ µM, Vmax of 0.378±0.036 µM/mg/min and Kcat of 4.78±0.021/min, indicating elevated levels of IMPDH activity under anaerobic conditions. Three-folds increased activity in the presence of 1 mM adenosine triphosphate (ATP) correlated with biofilm formation. The kinetics of pure rIMPDH were close to the native IMPDH of S. aureus ATCC12600 and the enzyme showed single band in sodium dodecyl sulphate polyacrylamide gel electrophoresis with a molecular weight of 53 KDa.Conclusions: Elevated activity of IMPDH was observed in S. aureus grown under anaerobic conditions and this was correlated with the biofilm formation indicating the linkage between purine metabolism and pathogenesis.

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Mechanisms of biofilm formation in Staphylococcus epidermidis and Staphylococcus aureus: functional molecules, regulatory circuits, and adaptive responses

Cited by 187 publications

References 92 publications

Lactobacillus acidophilus-derived biosurfactant effect on gtfB and gtfC expression level in Streptococcus mutans biofilm cells

Lactobacillus acidophilus-derived biosurfactant effect on gtfB and gtfC expression level in Streptococcus mutans biofilm cells

Comparison of antibacterial properties of commercial bone cements and fillers with a zinc-based glass polyalkenoate cement

Characterization of inosine monophosphate dehydrogenase from Staphylococcus aureus ATCC12600 and its involvement in biofilm formation

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