Lactobacillus acidophilus-derived biosurfactant effect on gtfB and gtfC expression level in Streptococcus mutans biofilm cells

Tahmourespour, Arezoo; Salehi, Rasoul; Kermanshahi, Rooha Kasra

doi:10.1590/s1517-83822011000100042

Cited by 65 publications

(54 citation statements)

References 45 publications

(25 reference statements)

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“…It was observed that neutralized SCS caused lower reduction in microbial growth than untreated SCS, but yet neutralized supernatant still showed significant reduction in Streptococcus mutans growth when compared to control. This suggests the influence of other antimicrobial agents such as hydrogen peroxide, bacteriocin, and biosurfactant that contribute with acid to growth inhibition.…”

Section: Discussionmentioning

confidence: 99%

“…supernatant‐induced altered gene expression indicates a promising anticaries effect. In vitro studies indicated that gtfB and gtfC were essential for the sucrose‐dependent attachment of Streptococcus mutans cells to hard surfaces and for microcolonies formation, but gtfD was not essential . In the biofilm‐forming bacteria, the expression of the three glucosyltransferase genes ( gtfB , gtfC and gtfD ) and the fructosyltransferase genes sacB ( ftf ) showed significant down‐regulation as compared to the control group of untreated biofilm‐forming cells.…”

Section: Discussionmentioning

confidence: 99%

ProbioticLactobacillussp. inhibit growth, biofilm formation and gene expression of caries-inducingStreptococcus mutans

et al. 2018

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Streptococcus mutans contributes significantly to dental caries, which arises from homoeostasic imbalance between host and microbiota. We hypothesized that Lactobacillus sp. inhibits growth, biofilm formation and gene expression of Streptococcus mutans. Antibacterial (agar diffusion method) and antibiofilm (crystal violet assay) characteristics of probiotic Lactobacillus sp. against Streptococcus mutans (ATCC 25175) were evaluated. We investigated whether Lactobacillus casei (ATCC 393), Lactobacillus reuteri (ATCC 23272), Lactobacillus plantarum (ATCC 14917) or Lactobacillus salivarius (ATCC 11741) inhibit expression of Streptococcus mutans genes involved in biofilm formation, quorum sensing or stress survival using quantitative real‐time polymerase chain reaction (qPCR). Growth changes (OD600) in the presence of pH‐neutralized, catalase‐treated or trypsin‐treated Lactobacillus sp. supernatants were assessed to identify roles of organic acids, peroxides and bacteriocin. Susceptibility testing indicated antibacterial (pH‐dependent) and antibiofilm activities of Lactobacillus sp. against Streptococcus mutans. Scanning electron microscopy revealed reduction in microcolony formation and exopolysaccharide structural changes. Of the oral normal flora, L. salivarius exhibited the highest antibiofilm and peroxide‐dependent antimicrobial activities. All biofilm‐forming cells treated with Lactobacillus sp. supernatants showed reduced expression of genes involved in exopolysaccharide production, acid tolerance and quorum sensing. Thus, Lactobacillus sp. can inhibit tooth decay by limiting growth and virulence properties of Streptococcus mutans.

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“…A major problem in many studies is the lack of evidence of complete purity or characterisation of the biosurfactant active fractions used, as in the case of the work carried out by ), Tahmourespour, Salehi and KasraKermanshahi (2011 and Salehi et al (2014) and their co-workers when they investigated the gene expression gtfB and gtfC of S. mutans, which are directly involved in biofilm matrix formation. In principle, these studies are unique in being the first to investigate oral-related bacterial gene expression, after treatment with biosurfactant.…”

Section: Potential Biosurfactant Applications For Oral Health and Hygmentioning

confidence: 99%

Biosurfactants: promising bioactive molecules for oral-related health applications

Elshikh

Marchant

Banat

2016

FEMS Microbiology Letters

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One sentence summary: Biosurfactant properties including antimicrobial activity, emulsion formation and the ability to increase bioavailability of hydrophobic compounds make them potential candidates for use in cosmetic, therapeutic, oral hygiene and medical devices. Editor: Geertje van Keulen ABSTRACTBiosurfactants are naturally produced molecules that demonstrate potentially useful properties such as the ability to reduce surface tensions between different phases. Besides having similar properties to their artificial chemical counterparts, they are regarded as environmental friendly, biodegradable and less toxic, which make them desirable candidates for downstream applications. The structure-activity-related properties of the biosurfactants which are directly correlated with potency of the biosurfactants as antimicrobial agents, the ability of the biosurfactants to alter surface energies and their ability to increase bioavailability are particularly what attract researchers to exploit their potential use in the oral-related health applications. Current research into biosurfactant indicates significant future potential for use in cosmetic and therapeutic oral hygiene product formulations and related medical device treatments.

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“…Antibiofilm glycolipids have also been isolated from Lactobacillus (Tahmourespour et al 2011;Zakaria Gomaa 2013). In this case, L. paracasei A20 produced biosurfactants that inhibit Gram-positive and Gram-negative bacteria, yeasts and filamentous fungi (Gudina et al 2010).…”

Section: Sophorolipidsmentioning

confidence: 99%

Microbial biofilms: biosurfactants as antibiofilm agents

Banat

Rienzo

Quinn

2014

Appl Microbiol Biotechnol

166

102

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Current microbial inhibition strategies based on planktonic bacterial physiology have been known to have limited efficacy on the growth of biofilm communities. This problem can be exacerbated by the emergence of increasingly resistant clinical strains. All aspects of biofilm measurement, monitoring, dispersal, control, and inhibition are becoming issues of increasing importance. Biosurfactants have merited renewed interest in both clinical and hygienic sectors due to their potential to disperse microbial biofilms in addition to many other advantages. The dispersal properties of biosurfactants have been shown to rival those of conventional inhibitory agents against bacterial and yeast biofilms. This makes them suitable candidates for use in new generations of microbial dispersal agents and for use as adjuvants for existing microbial suppression or eradication strategies. In this review, we explore aspects of biofilm characteristics and examine the contribution of biologically derived surface-active agents (biosurfactants) to the disruption or inhibition of microbial biofilms.

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“…Two probiotic bacteria Lactococcus lactis 53 and S. thermophilus A have been identifi ed as producers of cell-bound biosurfactants at the stationary growth phase in the presence of lactose and cheese whey as carbon substrates (Rodrigues et al 2006a , b ). A protein-containing biosurfactant from Lactobacillus acidophilus has also been demonstrated to reduce the adhesion and biofi lm formation of Streptococcus mutans on glass slides (Tahmourespour et al 2011 ), indicating that the treatment of teeth with this biosurfactant may be an alternative dental control for biofi lm development and adhesion of the pathogens on teeth. Similarly, Gudiña et al ( 2011 ) investigated the production of cell-bound and excreted biosurfactant by three lactobacilli strains and Leuconostoc mesenteroides strains.…”

Section: Glycolipidsmentioning

confidence: 99%

Production of Biosurfactants Using Eco-friendly Microorganisms

Uzoigwe

Ennis

Rahman

2014

Environmental Sustainability

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Pathogenicity of biosurfactant-producing microorganisms is currently raising some health, safety and environmental concerns. As a result, the industrial-scale production and application of biosurfactants as potential alternatives to the synthetic one is still an unachieved task. The production of biosurfactants using nonpathogenic/recombinant strains requires more attention and investigation for some advantages that includes the discovery of non-toxic biosurfactants suitable for all industrial applications, identifying new biosurfactant congeners with better inherent surfaceactive properties compared to that from pathogens and synthetic ones and the synthesis of biosurfactant without complex metabolic regulations. Although a number of nonpathogenic/recombinant, eco-friendly biosurfactant-producing strains have been documented, there is need for more research in this area focusing especially on improved biosurfactant production by these strains using optimisation processes and the discovery of new nonpathogenic/recombinant strains using molecular techniques for future sustainability.

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Lactobacillus acidophilus-derived biosurfactant effect on gtfB and gtfC expression level in Streptococcus mutans biofilm cells

Cited by 65 publications

References 45 publications

ProbioticLactobacillussp. inhibit growth, biofilm formation and gene expression of caries-inducingStreptococcus mutans

Biosurfactants: promising bioactive molecules for oral-related health applications

Microbial biofilms: biosurfactants as antibiofilm agents

Production of Biosurfactants Using Eco-friendly Microorganisms

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