Mechanisms of Adhesion by Pseudomonads

Hinsa, Shannon M.; O’Toole, George A.

doi:10.1007/978-1-4419-9086-0_23

Cited by 2 publications

(1 citation statement)

References 98 publications

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“…Long rupture separations, reaching up to 9 μm, were observed in some measurements (SI Figure S9), which can be explained by the presence of long flagella and pili in P. f luorescens. 15,54,56,92,93 For both PSF and PSF−PDA, longer rupture separations were recorded as t Contact was increased from 0 to 2 s, but remained unchanged on increasing t Contact to 5 s. The increase in average rupture separation as t Contact was varied from 0 to 2 s indicates that at longer t Contact , longer adhesins (possibly pili) mediated attachment or more contact points along a single pili were established. Beyond 2 s, the rupture separation did not vary significantly but the adhesion became stronger (Figure 2(A)), suggesting that more adhesins of similar length were involved.…”

Section: Environmental Science and Technologymentioning

confidence: 94%

Bacterial Adhesion to Ultrafiltration Membranes: Role of Hydrophilicity, Natural Organic Matter, and Cell-Surface Macromolecules

BinAhmed

Hasane

Wang

et al. 2017

Environ. Sci. Technol.

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Insight into the mechanisms underlying bacterial adhesion is critical to the formulation of membrane biofouling control strategies. Using AFM-based single-cell force spectroscopy, we investigated the interaction between Pseudomonas fluorescens, a biofilm-forming bacterium, and polysulfone (PSF) ultrafiltration (UF) membranes to unravel the mechanisms underlying early stage membrane biofouling. We show that hydrophilic polydopamine (PDA) coatings decrease bacterial adhesion forces at short bacterium-membrane contact times. Further, we find that adhesion forces are weakened by the presence of natural organic matter (NOM) conditioning films, owing to the hydrophilicity of NOM. Investigation of the effect of adhesion contact time revealed that PDA coatings are less effective at preventing bioadhesion when the contact time is prolonged to 2-5 s, or when the membranes are exposed to bacterial suspensions under stirring. These results therefore challenge the notion that simple hydrophilic surface coatings are effective as a biofouling control strategy. Finally, we present evidence that adhesion to the UF membrane surface is mediated by cell-surface macromolecules (likely to be outer membrane proteins and pili) which, upon contacting the membrane, undergo surface-induced unfolding.

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Section: Environmental Science and Technologymentioning

confidence: 94%

Bacterial Adhesion to Ultrafiltration Membranes: Role of Hydrophilicity, Natural Organic Matter, and Cell-Surface Macromolecules

BinAhmed

Hasane

Wang

et al. 2017

Environ. Sci. Technol.

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Pathogenic Arsenal of Pseudomonas Aeruginosa : An Update on Virulence Factors

Veetilvalappil

Manuel²,

Aranjani

et al. 2022

Future Microbiol.

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The emergence of Pseudomonas aeruginosa as a potential threat in persistent infections can be attributed to the plethora of virulence factors expressed by it. This review discusses the various virulence factors that help this pathogen to establish an infection and regulatory systems controlling these virulence factors. Cell-associated virulence factors such as flagella, type IV pili and non-pilus adhesins have been reviewed. Extracellular virulence factors have also been explained. Quorum-sensing systems present in P. aeruginosa play a cardinal role in regulating the expression of virulence factors. The identification of novel virulence factors in hypervirulent strains indicate that the expression of virulence is dynamic and constantly evolving. An understanding of this is critical for the better clinical management of infections.

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Mechanisms of Adhesion by Pseudomonads

Cited by 2 publications

References 98 publications

Bacterial Adhesion to Ultrafiltration Membranes: Role of Hydrophilicity, Natural Organic Matter, and Cell-Surface Macromolecules

Bacterial Adhesion to Ultrafiltration Membranes: Role of Hydrophilicity, Natural Organic Matter, and Cell-Surface Macromolecules

Pathogenic Arsenal of Pseudomonas Aeruginosa : An Update on Virulence Factors

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