Mechanisms of action of cyclic nucleotides on a bursting pacemaker and silent neuron in aplysia

Drake, Peter F.; Treistman, Steven N.

doi:10.1016/0006-8993(81)91304-4

Cited by 23 publications

(3 citation statements)

References 19 publications

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“…Changes in pacemaker activity have been also observed under the action of various inhibitors of phosphodiesterase on Aplysia and Helix bursting neurons (TREISTMAN and LEVITAN, 1976;KONONENKO, 1981;DRAKE and TREISTMAN, 1981). DRAKE and TREISTMAN (1981) reported that bath-applied 8BTcAMP induced the NSR region in the silent Aplysia neuron (motacerebral giant cell) in the same manner as IBMX. In molluscan neurons, at least three different ionic channels can be modulated by cAMP-dependent protein phosphorylation: the serotonin-sensitive potassium channel in the sensory neuron of Aplysia (CASTELLUCCI et al, 1982), the anomalous rectifying potassium channel in the R15 neuron of Aplysia (ADAMS and LEVITAN, 1982), and the A current of the peptidergic neurons of Aplysia (KACZMAREK and STRUMWASSER, 1984).…”

Section: Discussionmentioning

confidence: 94%

“…Cyclic nucleotide agents have been shown to dramatically alter the pacemaker activity (TREISTMAN and LEVITAN, 1976). Changes in pacemaker activity have been also observed under the action of various inhibitors of phosphodiesterase on Aplysia and Helix bursting neurons (TREISTMAN and LEVITAN, 1976;KONONENKO, 1981;DRAKE and TREISTMAN, 1981). DRAKE and TREISTMAN (1981) reported that bath-applied 8BTcAMP induced the NSR region in the silent Aplysia neuron (motacerebral giant cell) in the same manner as IBMX.…”

Section: Discussionmentioning

confidence: 99%

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Influences of pressure-injected cyclic AMP on the membrane current and characteristics of an identified neuron of Aplysia kurodai.

1985

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The ionic mechanism of the effect of intracellularly injected adenosine 3',5'-cyclic monophosphate (CAMP) on the membrane of identified neuron L5 of Apl ysia kurodai was investigated with conventional voltage-clamp and ion-substitution techniques. The intracellular elevation of CAMP caused an inward current ('CAMP), which was not accompanied by a significant change in membrane conductance at potentials more hyperpolarized than -60 mV. The current increased over the voltage range (-50 to -30 mV) associated with a conductance decrease and decreased at potentials more hyperpolarized than -60 mV. Elevated intracellular CAMP was found to enhance a region of negative slope resistance in steady-state I -V relations. Duration of the 'CAMP was greatly prolonged by bath-applied isobutylmethylxanthine (50 ,uM), but imidazole (10 mM) had an opposite effect on the 'CAMP. Tolbutamide (5 mM), a protein kinase inhibitor, reduced the DAMP. The current was not affected by the presence of bath-applied TTX (50 µM), ouabain (50 /LM), or triaminopyrimidine (5 mM). Reduction of [Na+]o reversibly decreased the DAMP. Li+ could largely substitute for Nat Alterations of [K+]o, and bath application of 4-AP (5 mM) and TEA (30 mM) did not affect the DAMP, In the presence of Nat, Cl-, and divalent cations such as Ca2+ and Ba2+ inhibited the DAMP. These results suggest that fast elevation of intracellular cAMP induces a TTX-resistant slow Na+ inward current, and the current might be due to activation of CAMP-dependent protein kinase.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Influences of pressure-injected cyclic AMP on the membrane current and characteristics of an identified neuron of Aplysia kurodai.

1985

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“…GLP-1 and other ␤-cell ion channels. Increases in intracellular cAMP have long been known to enhance Na ϩ currents (86), an effect that may be mediated through direct channel phosphorylation by PKA (87). A transient Na ϩ current response to cAMP was first described in gastropod neurons and termed I Na(cAMP) (88).…”

Section: Glp-1 and The ␤-Cellmentioning

confidence: 99%

The Multiple Actions of GLP-1 on the Process of Glucose-Stimulated Insulin Secretion

et al. 2002

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Serotonin and cAMP induce excitatory modulation of a serotonergic neuron

2006

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Serotonin (5-HT) is an excitatory neurotransmitter and neuromodulator. In the Aplysia nervous system it increases excitability and induces spike broadening in sensory neurons. It is released at the synaptic terminals of the metacerebral cells (MCCs) and modulates the feeding neural circuit and buccal muscles during the aroused feeding state. We report that MCC itself is depolarized by 5-HT and becomes excitable. 5-HT induces tonic spike activity and even spike-burst activity. Conceivably, this sensitivity to its own transmitter could provide positive feedback excitation of MCC. Voltage clamp analysis of isolated cultured MCCs shows that 5-HT reduces a calcium-dependent outward current at the resting potential (-60 mV), and enhances steady state inward currents between -55 and -30 mV and between -75 and -100 mV. 8-Br-cAMP has similar effects, suggesting that cAMP mediates the 5-HT effects, in part. A transient calcium current is enhanced at voltages more positive than -40 mV. Barium and cesium selectively block the 5-HT-induced inward current between -75 and -100 mV. Substitution of N-methyl-D-glucamine for sodium and adding cobalt block this current, also indicating that it is a hyperpolarization-activated cation current. The 5-HT-induced inward current between -55 and -30 mV is also blocked by sodium substitution and added cobalt, suggesting that 5-HT increases a depolarization-activated cation current. The outward current that remains when sodium and calcium currents are blocked is reduced by 5-HT. Thus, 5-HT enhances two different cation currents and reduces potassium currents.

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Mechanisms of action of cyclic nucleotides on a bursting pacemaker and silent neuron in aplysia

Cited by 23 publications

References 19 publications

Influences of pressure-injected cyclic AMP on the membrane current and characteristics of an identified neuron of Aplysia kurodai.

Influences of pressure-injected cyclic AMP on the membrane current and characteristics of an identified neuron of Aplysia kurodai.

The Multiple Actions of GLP-1 on the Process of Glucose-Stimulated Insulin Secretion

Serotonin and cAMP induce excitatory modulation of a serotonergic neuron

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