Mechanisms and Origin of Bacterial Biolumenescence

Zavilgelsky, G. B.; Шакулов, Р. С.

doi:10.1134/s0026893318060183

Cited by 7 publications

(14 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…aeruginosa lux strain (PAO1 Tn7::Plac-lux)] were provided by the Hancock laboratory, University of British Columbia, Vancouver. These bacteria contain a lux operon which encodes genes for the production of luminescent proteins . Metabolically active bacteria will be luminescent, and the luminescence activity is positively correlated with growth, which thus lowers upon bacterial growth inhibition.…”

Section: Methodsmentioning

confidence: 99%

“…aeruginosa lux strain (PAO1 Tn7::Plac-lux)] before making luminescence measurements on a Molecular Devices SpectraMax M3 plate reader. Luminescence measurements were taken as total counts observed over 50 ms for each well across all wavelengths . Statistical analysis was done using a one-way ANOVA test as described be below.…”

Section: Methodsmentioning

confidence: 99%

“…These bacteria contain a lux operon which encodes genes for the production of luminescent proteins. 71 Metabolically active bacteria will be luminescent, and the luminescence activity is positively correlated with growth, which thus lowers upon bacterial growth inhibition. Thereby, luminescence measurements were used as a measure of antibacterial activity of the compounds tested.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…Luminescence measurements were taken as total counts observed over 50 ms for each well across all wavelengths. 71 Statistical analysis was done using a one-way ANOVA test as described be below.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Polyglycerol-Based Macromolecular Iron Chelator Adjuvants for Antibiotics To Treat Drug-Resistant Bacteria

Abbina

Gill

Mathew

et al. 2020

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Iron is an essential micronutrient for life. Its redox activity is a key component in a plethora of vital enzymatic reactions that take place in processes such as drug metabolism, DNA synthesis, steroid synthesis, gene regulation, and cellular respiration (oxygen transport and the electron transport chain). Bacteria are highly dependent on iron for their survival and growth and have specific mechanisms to acquire iron. Limiting the availability of iron to bacteria, thereby preventing their growth, provides new opportunities to treat infection in the era of the persistent rise of antibiotic-resistant bacteria. In this work, we have developed macromolecular iron chelators, conjugates of a high-affinity iron chelator (HBEDS) with polyglycerol, in an attempt to sequester iron uptake by bacteria to limit their growth in order to enhance antibiotic activity. The new macromolecular chelators are successful in slowing the growth of Staphylococcus aureus and worked as an efficient bacteriostatic against S. aureus. Further, these cytocompatible macrochelators acted as effective adjuvants to prevent bacterial growth when used in conjunction with antibiotics. The adjuvant activity of the macrochelators depends on their molecular weight and the chelator density on these molecules. These selective macro iron(III) chelators are highly efficient in growth inhibition and killing of methicillin-resistant S. aureus in conjunction with a low concentration of rifampicin.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: ■ Materials and Methodsmentioning

confidence: 99%

Section: ■ Materials and Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Polyglycerol-Based Macromolecular Iron Chelator Adjuvants for Antibiotics To Treat Drug-Resistant Bacteria

Abbina

Gill

Mathew

et al. 2020

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…luxC, luxD and luxE constitute the fatty acid reductase complex, responsible for the synthesis of long Aldehyde substrate, luxG encodes avin reductase [2]. Conserved genes luxC, luxD, luxA, luxB, luxE, and luxG exist in all luminescent bacteria that have been discovered [3], in addition, there are other genes such as luxI, luxR and luxF [4]. Although luminescent bacteria have the same lux genes, these bacteria show great differences in characteristics such as growth behavior, luminescence intensity, or bioluminescence regulation [5].…”

Section: Introductionmentioning

confidence: 99%

Whole genome sequencing and bioinformatics analysis of Photobacterium kishitanii FJ21

Yin

Liu

et al. 2022

Preprint

View full text Add to dashboard Cite

Background: Photobacterium kishitanii FJ21 is isolated and purified in the laboratory, and it is a kind of gram-negative bacteria that can emit blue-green fluorescence under normal conditions. In order to understand the lux operon that has luminescence activity, the whole-genome sequencing and bioinformatics analysis of Photobacterium kishitanii were carried out by using Nanopore sequencing technology. The sequencing data were predicted by the secondary structure and tertiary structure of the protein encoded by lux genes, genome assembly, GC content, gene prediction and functional annotation, phylogenetic tree, genome collinearity analysis and secondary metabolite synthesis gene cluster prediction. Result: Sequencing results showed that there were luxC, luxD, luxA, luxB, luxF, luxE and luxG genes in Photobacterium kishitanii FJ21, and the protein encoded by lux genes had certain hydrophilicity. The genome of this strain contains a chromosome with a total length of 4853277bp and a GC content of 39.23%. There are 3141, 1769, 2472, 4070, 3514 and 1413 genes annotated in COG, KEGG, GO, Refseq, Pfam and TIGRFAMs databases, respectively. At the same time, three types of secondary metabolite gene clusters are predicted, namely RiPP-like, betalactone and arylpolyene. 870 genes were annotated in PHI database. The antibiotic resistance genes of the strain were annotated with CARD database. Conclusion: This study reported the whole genome sequence Photobacterium kishitanii FJ21, and the related research results will provide a basis for further study of lux genes, photogenic activities and mining of other functional genes of this strain.

show abstract

Pickering emulsion-enhanced Vibrio fischeri assay for ecotoxicity assessment of highly hydrophobic polycyclic aromatic hydrocarbons

Zhao

et al. 2023

Chemosphere

View full text Add to dashboard Cite

Mechanisms and Origin of Bacterial Biolumenescence

Cited by 7 publications

References 68 publications

Polyglycerol-Based Macromolecular Iron Chelator Adjuvants for Antibiotics To Treat Drug-Resistant Bacteria

Polyglycerol-Based Macromolecular Iron Chelator Adjuvants for Antibiotics To Treat Drug-Resistant Bacteria

Whole genome sequencing and bioinformatics analysis of Photobacterium kishitanii FJ21

Pickering emulsion-enhanced Vibrio fischeri assay for ecotoxicity assessment of highly hydrophobic polycyclic aromatic hydrocarbons

Contact Info

Product

Resources

About