Mechanism underlying transient gestational-onset hypothyroidism–induced impairment of posttesticular sperm maturation in adult rats

Jaganathan, Anbalagan; Sashi, A. M. J.; Ganapathy, Vengatesh; Stanley, Jone A.; Neelamohan, R; Aruldhas, M. M.

doi:10.1016/j.fertnstert.2010.02.005

Cited by 28 publications

(29 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…At the end of treatment period, each animal per group was anesthetized with chloroform and the blood samples were taken from the heart and then centrifuged to obtain sera. The levels of T4 and TSH in each serum were measured using chemiluminescent immunoassay [3].…”

Section: Thyroid Hormones Measurementmentioning

confidence: 99%

See 1 more Smart Citation

Assessment of sperm morphology, chromatin integrity, and catSper genes expression in hypothyroid mice

Alipour

Jalali

Nikravesh

et al. 2018

Acta Biologica Hungarica

View full text Add to dashboard Cite

There is an evident relationship between the fertilizing capacity of sperm and the normal morphology, quality chromatin, and motility of sperm. It is well known that thyroid hormones are the important regulators of testicular function. A correlation was found between the hypothyroidism and sperm damages. The present study was conducted to investigate the effects of hypothyroidism on sperm morphology, chromatin quality, and motility. For this purpose, 20 male mice were divided into the control and the hypothyroid groups that received 0.05% 6-n-propyl-2-thiouracil (PTU) for 35 days. Sperm morphology with Papanicolaou staining and sperm chromatin quality with both Aniline Blue (AB) and Toluidine blue (TB) staining were assessed. Besides, immunohistochemistry and real-time PCR were performed to evaluate the changes of cation sperm channel (CatSper) genes. A significant increase in the sperm chromatin condensation was found in the hypothyroid mice compared to the control mice (p < 0.05). Furthermore, a significant decrease was observed in the morphology of normal sperm in hypothyroid mice compared to the controls (p < 0.05). The results showed that Hypothyroidism could downregulate the expression of CatSper genes. Immunohistochemical data confirmed the real time-PCR results. Furthermore, the results showed that hypothyroidism could adversely affect sperm morphology, sperm chromatin condensation, and CatSper gene expression in mice and these abnormalities may be related to the excessive production of reactive oxygen species (ROS) in a hypothyroid state.

show abstract

Section: Thyroid Hormones Measurementmentioning

confidence: 99%

“…Several studies have shown that hypothyroidism decreases hyperactivated motility in sperm. Furthermore, a significant reduction has been observed in the progressive forward motility of sperm in the hypothyroid cases [3,16,17,33,37]. Sperm motility is considered to be one of the best predictors of fertilizing ability.…”

Section: Introductionmentioning

confidence: 99%

Assessment of sperm morphology, chromatin integrity, and catSper genes expression in hypothyroid mice

Alipour

Jalali

Nikravesh

et al. 2018

Acta Biologica Hungarica

View full text Add to dashboard Cite

show abstract

“…At least two studies were conducted on animal models. As discussed above, Anbalagan et al, (2010) reported adverse effects of gestational hypothyroidism on fertility of the upcoming generation in Wistar rats [48]. In another similar study, Maran and Aruldhas, (2002) reported adverse effects of hypothyroidism on male reproductive organs and fertility [67].…”

Section: Thyroid Disease and Male Fertilitymentioning

confidence: 82%

“…Gestational disturbance in thyroid function can affect not only testicular development of the upcoming generation but also its fertility potential in adulthood. Anbalagan et al, 2010, in a study on rats explored the mechanism underlying gestational onset hypothyroidism induced male infertility [48]. Pregnant rats were exposed to methimazole from embryonic days 9 to 14, 18, and 21, covering specific fetal periods of differentiation and development of male reproductive tract organs.…”

Section: Gestational Thyroid Activity Testicu-lar Development and Fementioning

confidence: 99%

“…Secretory activity of the epididymis was evaluated by quantifying sialic acid, carnitine, and glycerylphosphorylcholine. It was observed that gestational exposure to methimazole decreased sperm forward motility, in vivo fertilizing ability, bioavailability of androgens, AR status, and secretory activity of the epididymis in adult rats [48]. The authors concluded that transient gestational-onset hypothyroidism affects male fertility by impairing posttesticular sperm maturation process in the epididymis, owing to subnormal androgen(s) bioavailability, AR expression, and AR functional activity.…”

Section: Gestational Thyroid Activity Testicu-lar Development and Fementioning

confidence: 99%

See 1 more Smart Citation

Thyroid Hormones in Male Reproduction and Fertility

Rajender¹,

Hamada²,

Agarwal

2011

TORSJ

View full text Add to dashboard Cite

Thyroid hormones, previously thought not to affect spermatogenesis and male fertility, are now being recognized as having important role in spermatogenesis. The identification of thyroid hormone receptors on Sertoli cells, the nurturing cells for sperm in the testis, has embarked further research to investigate the role of thyroid hormones in male reproduction. Since spermatogenesis occurs in close contact with the Sertoli cells, the thyroid hormones must exert significant regulatory role in sperm production. Therefore, disturbances in the thyroid function could affect spermatogenesis and male fertility. Studies on human subjects and animals models are now revealing further insights into the effect of thyroid hormones on male fertility and infertility. The present review provides an update on the role of thyroid hormones in spermatogenesis and male fertility.

show abstract

Thyroid Hormone Regulation of Mammalian Reproductive Development and the Potential Impact of Endocrine-Disrupting Chemicals

Thoemke

Bastian

Anderson

2011

Endocrine Disruptors and Puberty

View full text Add to dashboard Cite

Mechanism underlying transient gestational-onset hypothyroidism–induced impairment of posttesticular sperm maturation in adult rats

Cited by 28 publications

References 27 publications

Assessment of sperm morphology, chromatin integrity, and catSper genes expression in hypothyroid mice

Assessment of sperm morphology, chromatin integrity, and catSper genes expression in hypothyroid mice

Thyroid Hormones in Male Reproduction and Fertility

Thyroid Hormone Regulation of Mammalian Reproductive Development and the Potential Impact of Endocrine-Disrupting Chemicals

Contact Info

Product

Resources

About