Mechanism study of intracellular zinc oxide nanocomposites formation

Król, Anna; Railean-Plugaru, Viorica; Złoch, Michał

doi:10.1016/j.colsurfa.2018.05.069

Cited by 54 publications

(37 citation statements)

References 42 publications

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“…The generated species reduce the formed Zn(OH) 2 leading to the development of (Zn[OH] 4 ) 2− and ZnO nuclei are formed, which agglomerate into ZnO nanoparticles according to the following mechanism (Equations ()–()). [ 27 ]

Zn {({CH}_{3} COO)}_{2} .2 H_{2} O \overset{aq . medium}{\to} Zn {(OH)}_{2} + 2 {CH}_{3} COOH

Zn {(OH)}_{2} + 2 {OH}^{-} \to {(Zn {[OH]}_{4})}^{- 2}

{(Zn {[OH]}_{4})}^{- 2} \to ZnO + H_{2} O + 2 {(OH)}^{-}

…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial ZnO Nanoparticle–Doped Polyvinyl Alcohol/Pluronic Blends as Active Food Packaging Films

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El‐Rehim

et al. 2020

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Today, plastic waste has been highlighted as one of the greatest threats to the environment. These environmental concerns and the increased necessity for safe food packaging have inspired scientists to focus on the development of active biodegradable materials. Herein, a novel poly(vinyl alcohol)/pluronic/ZnO nanocomposite film (PVA/PLUR/ZnO) is introduced as an active packaging material with enhanced antimicrobial activity. Gamma irradiation is used as a “green” route to prepare ZnO nanoparticles via a polymer pyrolysis method. The as‐prepared ecofriendly ZnO nanoparticles are characterized and incorporated into the PVA/PLUR matrix in different concentrations. Transmission electron microscopy and dynamic light scattering measurements prove that ZnO nanoparticles have a mean particle size of 30 nm with a spherical‐like morphology. Morphological and structural characterization confirm the successful incorporation of ZnO into the PVA/PLUR matrix, which in turn enhances the thermal and barrier properties of PVA/PLUR/ZnO nanocomposite films. On the other hand, the opacity of blends is increased. The PVA/PLUR/ZnO composites exhibit broad‐spectrum antimicrobial activity against Gram‐positive, Gram‐negative bacterial pathogens, and fungi, and the activity increases with increasing concentrations of ZnO nanoparticles. These results introduce PVA/PLUR/ZnO films as effective antimicrobial materials for active food‐packaging applications.

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Zn {({CH}_{3} COO)}_{2} .2 H_{2} O \overset{aq . medium}{\to} Zn {(OH)}_{2} + 2 {CH}_{3} COOH

Zn {(OH)}_{2} + 2 {OH}^{-} \to {(Zn {[OH]}_{4})}^{- 2}

{(Zn {[OH]}_{4})}^{- 2} \to ZnO + H_{2} O + 2 {(OH)}^{-}

…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial ZnO Nanoparticle–Doped Polyvinyl Alcohol/Pluronic Blends as Active Food Packaging Films

Amin

Partila

El‐Rehim

et al. 2020

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“…A number of microorganisms, including bacteria, fungi and yeast are investigated due to their efficiency in Zn 2+ sorption and ZnO NPs synthesis. Bacteria such as Lactobacillus produce ZnO NPs intracellularly [7]. Meanwhile, fungi and yeast such as Aspergillus aeneus and Pichia fermentans, respectively, produce ZnO NPs extracellularly [8,9].…”

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confidence: 99%

“…As Gram-positive bacteria, LAB have a thick cell wall consisting of peptidoglycan, lipoteichoic acid, protein, and polysaccharides [16]. The layers function as the sites for the biosorption and bioreduction of metal ions, due to their negative electrokinetic potential features, which attract the metal cations to initiate NPs biosynthesis [7]. Former study by Król et al [7] proposed that the deprotonated carboxyl groups (functional group) present on the cell surface of L. paracasei strain interact with the zincaqua complexes (electron acceptor) to form ZnO NPs.…”

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confidence: 99%

“…The layers function as the sites for the biosorption and bioreduction of metal ions, due to their negative electrokinetic potential features, which attract the metal cations to initiate NPs biosynthesis [7]. Former study by Król et al [7] proposed that the deprotonated carboxyl groups (functional group) present on the cell surface of L. paracasei strain interact with the zincaqua complexes (electron acceptor) to form ZnO NPs. Also, LAB produce a wide range of exopolysaccharides (EPS) [17], which serve as the additional binding sites for the cationic metal ions and protect the microorganisms from toxic metal stresses [18].…”

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Sustainable microbial cell nanofactory for zinc oxide nanoparticles production by zinc-tolerant probiotic Lactobacillus plantarum strain TA4

2020

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Background: The use of microorganisms in the biosynthesis of zinc oxide nanoparticles (ZnO NPs) has recently emerged as an alternative to chemical and physical methods due to its low-cost and eco-friendly method. Several lactic acid bacteria (LAB) have developed mechanisms in tolerating Zn 2+ through prevention against their toxicity and the production of ZnO NPs. The LAB's main resistance mechanism to Zn 2+ is highly depended on the microorganisms' ability to interact with Zn 2+ either through biosorption or bioaccumulation processes. Besides the inadequate studies conducted on biosynthesis with the use of zinc-tolerant probiotics, the understanding regarding the mechanism involved in this process is not clear. Therefore, this study determines the features of probiotic LAB strain TA4 related to its resistance to Zn 2+. It also attempts to illustrate its potential in creating a sustainable microbial cell nanofactory of ZnO NPs. Results: A zinc-tolerant probiotic strain TA4, which was isolated from local fermented food, was selected based on the principal component analysis (PCA) with the highest score of probiotic attributes. Based on the 16S rRNA gene analysis, this strain was identified as Lactobacillus plantarum strain TA4, indicating its high resistance to Zn 2+ at a maximum tolerable concentration (MTC) value of 500 mM and its capability of producing ZnO NPs. The UV-visible spectroscopy analysis proved the formations of ZnO NPs through the notable absorption peak at 380 nm. It was also found from the dynamic light scattering (DLS) analysis that the Z-average particle size amounted to 124.2 nm with monodisperse ZnO NPs. Studies on scanning electron microscope (SEM), energy-dispersive X-ray (EDX) spectroscopy, and Fourier-transform infrared spectroscopy (FT-IR) revealed that the main mechanisms in ZnO NPs biosynthesis were facilitated by the Zn 2+ biosorption ability through the functional groups present on the cell surface of strain TA4. Conclusions: The strong ability of zinc-tolerant probiotic of L. plantarum strain TA4 to tolerate high Zn 2+ concentration and to produce ZnO NPs highlights the unique properties of these bacteria as a natural microbial cell nanofactory for a more sustainable and eco-friendly practice of ZnO NPs biosynthesis.

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“…bacterial proteins. Our previous work [37] have shown that addition of 3 mM zinc nitrate to the Lactobacillus paracasei LB3 cell suspension have resulted in the intracellular formation of zinc oxide nanocomposites which could also form aggregates and be observed as a multiplied signals on the CE electropherogram. According to their data from FT-IR analysis, the main groups involved in the ZnO biosynthesis are carboxyl and amid groups derived from bacterial proteins.…”

Section: Resultsmentioning

confidence: 99%

Electrophoretic Determination of Lactococcus lactis Modified by Zinc Ions

2018

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The developed capillary electrophoresis (CE) method was applied to evaluate the aggregation activity of divalent metal ions (zinc) on bacterial cells. In our study, the influence of the Lactococcus lactis surface modification by zinc ions at different concentration (1, 3 and 10 mM, respectively) was determined. Electrophoretic results in pseudo isotachophoretic mode indicated that using a Zn aq 2+ caused the uncontrolled aggregation of bacterial cells and, as a result, new signals of microbial agglomerates were observed in the electropherogram. Moreover, the observed process is strongly related with the concentration of the used Zn aq 2+ solution-the number of bacterial agglomerates increased with an increasing concentration of modifier. The phenomenon of L. lactis uncontrolled clumping was also evaluated by application of size distribution and spectrometric in infrared range measurements (FT-IR) measurements. The obtained data indicated that the main groups involved in the bacterial cells aggregation process are deprotonated carboxyl and amid groups derived from microbial surface proteins. As a consequence, the proposed mechanism of the L. lactis uncontrolled clumping process is presented. In addition, data from fluorescence microscopy pointed out damage cells analyzed direct after electroanalysis. Therefore, CE may be a potential method for evaluating the aggregation activity of different types of bivalent metal ions against microbial cells.

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Mechanism study of intracellular zinc oxide nanocomposites formation

Cited by 54 publications

References 42 publications

Antimicrobial ZnO Nanoparticle–Doped Polyvinyl Alcohol/Pluronic Blends as Active Food Packaging Films

Antimicrobial ZnO Nanoparticle–Doped Polyvinyl Alcohol/Pluronic Blends as Active Food Packaging Films

Sustainable microbial cell nanofactory for zinc oxide nanoparticles production by zinc-tolerant probiotic Lactobacillus plantarum strain TA4

Electrophoretic Determination of Lactococcus lactis Modified by Zinc Ions

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