“…Using Flp-In T-REx 293 cells, we generated a stable cell line that constitutively expressed human TEFM as a fusion protein containing a carboxyterminal BirA*-HA tag (TEFM-BirA*). As expected, our results confirmed that TEFM interacts with POLRMT ( Fig 6), as previously reported [16,17,19]. In addition, we identified a number of RNA processing-related proteins that interacted with TEFM, including the RNase P complex (MRPP1-3), GRSF1, FASTKD5, ELAC2, mtPAP, and the components of mitochondrial RNA degradosome (SUPV3L1-PNPase) complex (Fig 6), which are all reported to be localized in or adjacent to RNA granules [29,30,37,40,41,57].…”