Mechanisms of mammalian mitochondrial transcription

Bouda, Emilie; Stapon, Anthony; García‐Díaz, Miguel

doi:10.1002/pro.3688

Cited by 21 publications

(22 citation statements)

References 130 publications

(264 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The transcription of mtDNA relies entirely on nuclear-encoded factors [16]. Mitochondrial RNA polymerase (POLRMT), the main player in this process, is a DNA-dependent RNA polymerase which is highly similar to phage T7 polymerase [17][18][19].…”

Section: Mitochondrial Transcriptionmentioning

confidence: 99%

Mitochondrial OXPHOS Biogenesis: Co-Regulation of Protein Synthesis, Import, and Assembly Pathways

Tang

Thompson

Taylor

et al. 2020

IJMS

View full text Add to dashboard Cite

The assembly of mitochondrial oxidative phosphorylation (OXPHOS) complexes is an intricate process, which—given their dual-genetic control—requires tight co-regulation of two evolutionarily distinct gene expression machineries. Moreover, fine-tuning protein synthesis to the nascent assembly of OXPHOS complexes requires regulatory mechanisms such as translational plasticity and translational activators that can coordinate mitochondrial translation with the import of nuclear-encoded mitochondrial proteins. The intricacy of OXPHOS complex biogenesis is further evidenced by the requirement of many tightly orchestrated steps and ancillary factors. Early-stage ancillary chaperones have essential roles in coordinating OXPHOS assembly, whilst late-stage assembly factors—also known as the LYRM (leucine–tyrosine–arginine motif) proteins—together with the mitochondrial acyl carrier protein (ACP)—regulate the incorporation and activation of late-incorporating OXPHOS subunits and/or co-factors. In this review, we describe recent discoveries providing insights into the mechanisms required for optimal OXPHOS biogenesis, including the coordination of mitochondrial gene expression with the availability of nuclear-encoded factors entering via mitochondrial protein import systems.

show abstract

Section: Mitochondrial Transcriptionmentioning

confidence: 99%

Mitochondrial OXPHOS Biogenesis: Co-Regulation of Protein Synthesis, Import, and Assembly Pathways

Tang

Thompson

Taylor

et al. 2020

IJMS

View full text Add to dashboard Cite

show abstract

“…We first tested if mitochondrial activity is required for alveologenesis by inactivating Tfam (transcription factor A, mitochondria), which encodes a master regulator of mitochondrial transcription (20), in the mouse lung after birth. We produced CAGG CreER/+ ; ROSA26 mTmG/+ (control) and Tfam f/f ; CAGG CreER/+ ; ROSA26 mTmG/+ mice.…”

Section: Postnatal Removal Of Mitochondrial Activity In the Lung Leads To Defective Alveologenesismentioning

confidence: 99%

Acquisition of cellular properties during alveolar formation requires differential activity and distribution of mitochondria

Chuang

K²,

Yao³

et al. 2021

Preprint

View full text Add to dashboard Cite

Alveolar formation requires coordinated movement and interaction between alveolar epithelial cells, mesenchymal myofibroblasts and endothelial cells/pericytes to produce secondary septa. These processes rely on the acquisition of distinct cellular properties to enable ligand secretion for cell-cell signaling and initiate morphogenesis through cell migration and cell shape change. In this study, we showed that mitochondrial activity and distribution play a key role in bestowing cellular functions on both alveolar epithelial cells and mesenchymal myofibroblasts for generating secondary septa to form alveoli. These results suggest that mitochondrial function is tightly regulated to empower cellular machineries in a spatially specific manner. Indeed, such regulation via mitochondria is required for secretion of platelet-derived growth factor from alveolar epithelial cells to influence myofibroblast proliferation and migration. Moreover, mitochondrial function enables myofibroblast migration during alveolar formation. Together, these findings yield novel mechanistic insights into how mitochondria regulate pivotal steps of alveologenesis. They highlight selective utilization of energy and diverse energy demands in different cellular processes during development. Our work serves as a paradigm for studying how mitochondria control tissue patterning.

show abstract

“…The detailed view of transcription initiation will be complemented by a comprehensive picture that will highlight multiple feedback events between the mitochondria and nucleus that are necessary for regulating mtDNA transcription. Mammalian mtDNA transcription and regulation has been reviewed in many excellent articles ( 11 , 12 , 13 , 14 ). Herein, we will highlight recent studies that further enhance our understanding of the regulation of mammalian mtDNA transcription.…”

Section: Brief Overview Of Mitochondrial Functionsmentioning

confidence: 99%

“…5 , path C ) and represents another form of mitochondrial transcription regulation (recently reviewed in Ref. 14 ).…”

Section: Regulation Of Mitochondrial Transcriptionmentioning

confidence: 99%

Structure, mechanism, and regulation of mitochondrial DNA transcription initiation

Basu

Bostwick

Das

et al. 2020

Journal of Biological Chemistry

View full text Add to dashboard Cite

Mitochondria are specialized compartments that produce requisite ATP to fuel cellular functions and serve as centers of metabolite processing, cellular signaling, and apoptosis. To accomplish these roles, mitochondria rely on the genetic information in their small genome (mitochondrial DNA) and the nucleus. A growing appreciation for mitochondria's role in a myriad of human diseases, including inherited genetic disorders, degenerative diseases, inflammation, and cancer, has fueled the study of biochemical mechanisms that control mitochondrial function. The mitochondrial transcriptional machinery is different from nuclear machinery. The in vitro reconstituted transcriptional complexes of Saccharomyces cerevisiae (yeast) and humans, aided with high-resolution structures and biochemical characterizations, have provided a deeper understanding of the mechanism and regulation of mitochondrial DNA transcription. In this review, we will discuss recent advances in the structure and mechanism of mitochondrial transcription initiation. We will follow up with recent discoveries and formative findings regarding the regulatory events that control mitochondrial DNA transcription, focusing on those involved in crosstalk between the mitochondria and nucleus.

show abstract

Mechanisms of mammalian mitochondrial transcription

Cited by 21 publications

References 130 publications

Mitochondrial OXPHOS Biogenesis: Co-Regulation of Protein Synthesis, Import, and Assembly Pathways

Mitochondrial OXPHOS Biogenesis: Co-Regulation of Protein Synthesis, Import, and Assembly Pathways

Acquisition of cellular properties during alveolar formation requires differential activity and distribution of mitochondria

Structure, mechanism, and regulation of mitochondrial DNA transcription initiation

Contact Info

Product

Resources

About