Mechanism of the Decreased Erythropoiesis in the Water Deprived Rat

Giglio, J. M.; Alippi, Rosa M.; Barceló, Ana C.; Bozzini, Carlos E.

doi:10.1111/j.1365-2141.1979.tb03701.x

Cited by 3 publications

(1 citation statement)

References 11 publications

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“…Since the disappearance rate of EPO from the plasma compartment was normal and no inhibitor was detectable in the plasma of starved rats, the authors concluded that starvation decreases the EPO-sensitive progenitor cell pool. Studies performed in our laboratory confirmed these findings and extended them to the water-deprived, polycythemic rat [3]. Furthermore, they also showed that the ability of rats to respond normally to EPO is dependent on a continuous dietary intake of proteins at levels which are dependent on their biological values [4].…”

Section: Introductionsupporting

confidence: 68%

Impaired Response of Polycythemic Mice to Erythropoietin Induced by Protein Starvation Imposed After Hormone Administration

et al. 1993

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The present study was performed to determine the stage of the erythropoietic pathway which is affected by starvation or protein deprivation and whose manifestation is a depressed response to exogenous erythropoietin (EPO). The response to recombinant human EPO was measured in post-hypoxic polycythemic mice by determination of 59Fe uptake into red cells, spleen and femur and/or erythroid colony forming units (CFU-E) and erythroid precursor cell concentrations in femoral marrow. Experimental mice were either starved or fed one of seven different diets whose protein (casein) content ranged from 0 to 20%. All diets were isocaloric. The response of mice maintained on the standard diet (Purina Lab chow) was taken as the normal one. Starvation during the 48-hour period immediately before EPO injection had no effect on the response to the hormone. Starvation, and protein deprivation to a lesser extent, during the 48-hour period following EPO, on the other hand, significantly reduced the response. There was a progressive increase in the response as the casein content of the diet was increased. A normal response was observed when dietary casein concentration was 10%. These findings indicate that nutritional deprivation or dietary protein alterations during the period immediately following EPO injection in polycythemic mice can have detrimental effects on the erythroid response in a model in which nutritional deprivation was relatively short and acute. They also indicate that the subnormal response is not due to a decreased size of the erythroid progenitor pool available for differentiation but to deficient rates of differentiation of erythropoietic units.

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Section: Introductionsupporting

confidence: 68%

Impaired Response of Polycythemic Mice to Erythropoietin Induced by Protein Starvation Imposed After Hormone Administration

et al. 1993

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Erythropoietin levels can be used to determine tissue oxygenation in critically ill patients

Padayatty¹

1998

Medical Hypotheses

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Influence of Dietary Protein Concentration and Quality on Response to Erythropoietin in the Polycythaemic Rat

Alippi¹,

Giglio²,

Barceló³

et al. 1979

Br J Haematol

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Summary. The effects were examined of dietary protein concentration and quality on the response of polycythaemic hypertransfused rats to 6 units of human urinary erythropoietin. Rats were either starved or fed one of 14 different diets. Four protein sources were used, having a quality gradient from 100 to about 24. Two proteins—casein and wheat gluten—were used at five different levels of concentration (5–25%) in the diet. The response of rats maintained on the standard diet (Purina rat chow, 23.4% protein/g) was taken as the normal standard. The response to erythropoietin was 25% of normal in starved rats and 35% of normal in rats put on a protein‐free diet. When 10% protein in the diet was obtained by using high biological value proteins (egg yolk or casein) the response to erythropoietin was normal. When the same concentration was achieved by using low biological value proteins (wheat gluten or corn protein) the response to erythropoietin was undistinguishable from that of rats put on the protein‐free diet. When rats were maintained on diets with different concentrations of casein (5–25%) a normal response was observed when protein concentration was 10% with no further changes at higher concentrations. When rats were fed diets with different wheat gluten concentrations (5–25%) the response to erythropoietin was subnormal. These data suggest that the ability of rats to respond normally to erythropoietin is dependent on a continuous dietary intake of proteins at levels which are dependent on their biological values.

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Mechanism of the Decreased Erythropoiesis in the Water Deprived Rat

Cited by 3 publications

References 11 publications

Impaired Response of Polycythemic Mice to Erythropoietin Induced by Protein Starvation Imposed After Hormone Administration

Impaired Response of Polycythemic Mice to Erythropoietin Induced by Protein Starvation Imposed After Hormone Administration

Erythropoietin levels can be used to determine tissue oxygenation in critically ill patients

Influence of Dietary Protein Concentration and Quality on Response to Erythropoietin in the Polycythaemic Rat

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