Mechanism of Tetracycline Resistance in Staphylococcus aureus

Sompolinsky, D; Zaidenzaig, Yeshayahu; Ziegler-Schlomowitz, Ruth; Абрамова, Н В

doi:10.1099/00221287-62-3-351

Cited by 39 publications

(20 citation statements)

References 5 publications

(8 reference statements)

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“…Tetracycline uptake has been shown to be an energy-requiring system in E. coli (6) and S. aureus (9). This was confirmed to be true also in P. aeruginosa (Fig.…”

Section: Resultssupporting

confidence: 53%

“…It would be of interest to examine a variety of characteristics of the various streptomycin phosphotransferases to determine their degree of relatedness and thus the probability of common origin. In the case of R factor or episomal tetracycline resistance, there is evidence reported here and elsewhere (6,9) …”

Section: Downloaded Fromsupporting

confidence: 55%

“…Thus R931 appears to be of unique origin relative to previously studied R factors. The mechanisms of R931-mediated streptomycin and tetracycline resistance are of considerable interest for purposes of comparison with those mediated by other plasmids (6,9). Such information could be used as clues to the origin of r-determinants carried on diverse R factors (5).…”

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confidence: 99%

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Mechanisms of R Factor R931 and Chromosomal Tetracycline Resistance in Pseudomonas aeruginosa

Tseng

Bryan

1973

Antimicrob Agents Chemother

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The mechanism of tetracycline resistance mediated by R931 (a Pseudomonas aeruginosa R factor not yet successfully transferred to Escheri-chia coli recipients) was examined. In strain 931 (R931) (minimal inhibitory concentration [MIC] 200 ug/ml) significant tetracycline uptake did not occur until 100 ug of tetracycline per ml was included in uptake studies. The introduction of R931 into strain 280 resulted in a significant decline in 3H-tetracycline uptake. In both strains 931 (R931) and 280 (R931), a further reduction in tetracycline uptake resulted from pre-incubation with 1 ug of tetracycline per ml. Tetracycline resistance in R-P. aeruginosa strains 1731, 1885, and 494, considered to be of chromosomal origin, was associated with a lack of tetracycline uptake until the MIC of the strain was obtained. No evidence of tetracycline inactivation or ribosomal resistance was detected in R-or R+ strains. The MIC for R-strains was generally about 25 pg/ml and that for R+ strains was 75 to 200 Ag/ml. The R factor 931 is an example of a group of R factors detected in strains of Pseudomonas aeruginosa, which are unusual in that they fail to transfer to Escherichia coli or Proteus mirabilis but act as a naturally occurring, high-frequency transfer system when P. aeruginosa recipients are used in mating (4). Thus R931 appears to be of unique origin relative to previously studied R factors. The mechanisms of R931-mediated streptomycin and tetracycline resistance are of considerable interest for purposes of comparison with those mediated by other plasmids (6, 9). Such information could be used as clues to the origin of r-determinants carried on diverse R factors (5). It may also be of use in design of drugs effective against R factor-mediated antimicrobial resistance. The mechanism of R931-mediated streptomycin resistance has been shown to be phosphorylation of streptomycin (3). The mode of plasmid or chromosomal tetracycline resistance is unknown in P. aeruginosa. The results of an examination of such tetracycline resistance are reported in this paper.

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“…Tetracycline uptake has been shown to be an energy-requiring system in E. coli (6) and S. aureus (9). This was confirmed to be true also in P. aeruginosa (Fig.…”

Section: Resultssupporting

confidence: 53%

Section: Downloaded Fromsupporting

confidence: 55%

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Mechanisms of R Factor R931 and Chromosomal Tetracycline Resistance in Pseudomonas aeruginosa

Tseng

Bryan

1973

Antimicrob Agents Chemother

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“…This type of growth curve in which the lag period varies with the drug concentration is seen when an inhibiting drug is inactivated by the culture (Sompolinsky & Samra, 1968). However, no clear evidence for inactivation was found experimentally (Sompolinsky, Zaidenzaig, Ziegler-Schlomowitz & Abramova, 1970).…”

Section: Resultsmentioning

confidence: 99%

Inducible Resistance to Tetracycline in Staphylococcus aureus

Sompolinsky

Krawitz

Zaidenzaig

et al. 1970

Journal of General Microbiology

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S U M M A R YWhen staphylococci, resistant to 4 pg. tetracycline/ml., were grown in nutrient media at subinhibitory levels of the drug, phenotypical resistance increased until the cocci grew with 160 pg. tetracyclinelml. Resistance increased most rapidly at the highest concentration of tetracycline which did not significantly inhibit growth. Increase in resistance was also obtained by pre-incubation with p-apo-5-oxy-tetracycline. Increase in resistance could be prevented by chloramphenicol and actinomycin D, but not by nalidixic acid. When a highly resistant culture was transferred to tetracycline-free medium, phenotypical resistance decreased gradually ; after four transfers on nutrient agar it returned entirely to the original level. I N T R O D U C T I O NWilliams (1967) has drawn attention to the resistance to mercury salts (Moore, I 960) of tetracycline-resistant strains of Staphylococcus aureus. Resistance to tetracycline was in general correlated with resistance to mercury; in mercury-sensitive strains, however, high-level resistance to tetracycline could be obtained by ' training ', i.e. growth in peptone water containing 3 pg. antibiotic/ml. METHODSBacterial strains. The strain studied, Staphjdococcus aureus I I I (Sompolinsky, Yiflah & Aboud, 1568), was susceptible to mercuiy salts (Moore, 1960) and resistant to tetracycline (Tc) by virtue of a plasmid that could be eliminated by growth at 44" (May, Houghton & Perret, 1964). The eliminated susceptible strain grew on nutrient agar containing 0.2 pg. Tc/ml. ; on agar containing 0.4 pg. Tc/ml., colonies did not develop from an inoculum of IO* staphylococci. With the uneliminated culture, identical colony counts were obtained on plain agar and on agar containing 4-0 pg. Tc/ml. At concentrations of up to 160pg. Tc/ml., some colonies developed but growth did not occur on nutrient agar containing 320pg. Tc/ml. Three types of Iesistant cultures have been used: (a) a culture transferred daily on plain nutrient agar; only 5 to 10% of the viable cocci from this culture produced colonies on agar containing 10 pg. Tc/ml. ; (b) a highly resistant culture subcultured daily on nutrient agar containing I 60 pg. Tc/ml.; and (c) cultures with intermediate levels of resistance obtained by growth of type (a) culture for a short period in nutrient broth containing tetracycline at different concentrations.

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“…In some cases, R plasmids are known to modify the permeability of bacterial cells. For example, plasmid-controlled resistance to tetracycline in Staphylococcus aureus and Escherichia coli is due to decreased uptake of the drug (Sompolinsky et al, 1970;Franklin, 1967).…”

Section: Discussionmentioning

confidence: 99%