1991
DOI: 10.1084/jem.174.6.1527
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Mechanism of target cell recognition by natural killer cells: characterization of a novel triggering molecule restricted to CD3- large granular lymphocytes.

Abstract: Sllmmal-yIn an attempt to identify a molecule in target recognition by CD3-large granular lymphocytes (LGL), we have generated a rabbit antiidiotypic (anti-ID) serum against a monoclonal antibody (mAb 36) that reacted with the cell membrane of K562. Flow cytometry analysis demonstrated that the anti-ID serum bound selectively to CD3-LGL and that F(ab')2 fragments of the anti-ID serum blocked both target cell binding and lysis by NK cells. Stimulation of CD3-LGL with F(ab')z fragments resulted in the release of… Show more

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Cited by 41 publications
(18 citation statements)
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References 35 publications
(36 reference statements)
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“…A synthetic peptide corresponding to a positively charged region of the predicted NK-2 fusion protein (peptide 104; residues 476-497 in Fig. 1) was injected into rabbits and produced the anti-p104 antiserum that reacted with the 150-kDa molecule (12). The antipeptide antiserum possessed properties similar to the original anti-ID antiserum.…”
Section: Resultsmentioning
confidence: 99%
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“…A synthetic peptide corresponding to a positively charged region of the predicted NK-2 fusion protein (peptide 104; residues 476-497 in Fig. 1) was injected into rabbits and produced the anti-p104 antiserum that reacted with the 150-kDa molecule (12). The antipeptide antiserum possessed properties similar to the original anti-ID antiserum.…”
Section: Resultsmentioning
confidence: 99%
“…The anti-ID antiserum was shown by immunofluorescence to bind specifically to CD3, CD16+ NK cells, and it blocked the binding of LGL to NK-susceptible targets, thereby preventing lysis (12). Two proteins of 80 and 150 kDa were immunoprecipitated from 125I surface-labeled NK cells but not from T cells or monocytes.…”
mentioning
confidence: 99%
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“…Most recently, CD3-large granular lymphocytes have been reported to have a cell surface molecule involved in both recognition and triggering natural killer cell-mediated lytic function (43), which, when cloned and sequenced, has 50%6 homology to cyclophilin (S. K. Anderson, S. Gallinger, J. Roder, J. Frey, H. Young, and J. Ortaldo, personal communication).…”
Section: Discussionmentioning
confidence: 99%
“…15,52 In natural-killer cells, CypNK was identified as a component of the putative tumour recognition complexes. 52,53 Although a direct role for CypNK in target-cell recognition has not been demonstrated, CypNK may be a useful tool in the molecular basis of tumourcell recognition. 15 Cyclophilin 20 (Cyp20) is a less abundant, mitochondrially located, cyclophilin.…”
Section: General Functions Of Cypsmentioning
confidence: 99%