Mechanism of Tang Luo Ning effect on attenuating of oxidative stress in sciatic nerve of STZ-induced diabetic rats

Yang, Xinwei; Yao, Weijie; Li, Qingqin; Liu, Haolong; Shi, Haotian; Gao, Yanbin; Xu, Liping

doi:10.1016/j.jep.2015.07.047

Cited by 25 publications

(35 citation statements)

References 39 publications

(30 reference statements)

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“…Moreover, SCs play an extensive role in the regulation of axonal regeneration, hyperglycemia-induced SCs damages may cause reduced nerve conductivity, axonal atrophy, and impaired axonal regeneration 31 . In our previous study, TLN was found to improve the deficiency of motor nerve conductivity, sensory nerve conductivity and thresholds of thermal perception which were accompanied by lesser demyelination and a milder axonal atrophy in DPN rats 23 . It was also found to induce apoptosis in hyperglycemic media 32 .…”

Section: Discussionmentioning

confidence: 80%

“…Our previous study demonstrated that TLN improves oxidative stress by upregulating the expression of Nrf2 in STZ-induced DPN rats 23 . Nrf2 is a direct PERK substrate, and contributes to cellular redox homeostasis by regulating phase II antioxidant response 24 .…”

Section: Resultsmentioning

confidence: 93%

“…Briefly, the following crude herbs were combined to make the TLN extract powder: 15.0 g of Huangqi, 15.0 g of Danshen, 15.0 g of Jixueteng, 15.0 g of Mugua, 15.0 g of Gouji, 12.0 g of Chishao, 12.0 g of Niuxi and 10.0 g of Yanhusuo. The protocol of preparation TLN powder was as previously described 23 and the powder was dissolved in distilled water.…”

Section: Methodsmentioning

confidence: 99%

“…Male Sprague-Dawley (SD) rats (weighing 200 ± 20 g) were obtained from the Experimental Animal Center at Capital Medical University, Beijing, China (SCXK 2012–0001) and were housed on a 12 hours on and 12 hours off light cycle at 23 ± 2 °C in rooms with 55 ± 10% relative humidity and allowed free access to drinking water and standard laboratory chows. Experimental design, protocols and sample collections were the same as previously described 23 . In brief, rats were randomly divided into normal, DPN, α-lipoic acid (ALA), low dose TLN (LTLN) and high dose TLN (HTLN) groups.…”

Section: Methodsmentioning

confidence: 99%

“…Traditional Chinese medicine Tangluoning (TLN) based on Huangqi Guizhi Wuwu decoction is clinically used to treat DPN in China 22 . Our previous study has shown that TLN markedly improves the neurological functions including thermal perception threshold and nerve conductivity in DPN rats by attenuating oxidative stress through the activation of Nrf2 23 . It is possible that TLN may affect ER stress sensor PERK and attenuate DPN through modulating oxidative and ER stress.…”

Section: Introductionmentioning

confidence: 95%

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Tangluoning, a traditional Chinese medicine, attenuates in vivo and in vitro diabetic peripheral neuropathy through modulation of PERK/Nrf2 pathway

Yang

Yao

Liu

et al. 2017

Sci Rep

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Prolonged hyperglycemia-induced oxidative stress and endoplasmic reticulum stress have been demonstrated to play a key role in progression of diabetic peripheral neuropathy (DPN). PERK/ Nrf2 pathway plays a predominant role in oxidative and endoplasmic reticulum (ER) stress which is associated with cell survival. This study examined the modulation of the PERK/Nrf2 pathway and apoptosis by a traditional Chinese medicine Tangluoning (TLN) in streptozotocin-induced DPN rat models and the effects of serum TLN on the PERK/Nrf2 pathway, apoptosis, intracellular reactive oxygen species and mitochondrial membrane potential in Schwann cells cultured in 150 mM glucose. It is found that TLN attenuated oxidative and ER stress and apoptosis through the PERK/Nrf2 pathway by upregulating p-PERK, Nrf2/ARE pathways and downregulating the CHOP-related apoptosis pathways in the experimental DPN models both in vivo and in vitro.

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Section: Discussionmentioning

confidence: 80%

Section: Resultsmentioning

confidence: 93%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

See 3 more Smart Citations

Tangluoning, a traditional Chinese medicine, attenuates in vivo and in vitro diabetic peripheral neuropathy through modulation of PERK/Nrf2 pathway

Yang

Yao

Liu

et al. 2017

Sci Rep

Self Cite

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Effects of Tang Luo Ning on diabetic peripheral neuropathy in rats revealed by LC–MS metabolomics approach

Yao²,

Gao

2022

Biomedical Chromatography

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Diabetic peripheral neuropathy (DPN) is one of the most common complications of diabetes with limited therapies. Tang Luo Ning (TLN), a traditional Chinese medicine compound, has been proved to be effective in the treatment of DPN in clinical and experimental studies. However, the potential metabolic mechanism of TLN for the treatment of DPN is still unclear. Here the therapeutic effect of TLN on DPN was studied, and HPLC‐IT‐TOF/MS was used to explore the metabolic changes related to DPN and to explore the mechanism of TLN on DPN induced by high glucose. Furthermore, metabolic pathway analysis was used to explore the metabolic changes induced by DPN and TLN. As a result, TLN could improve the peripheral nerve function of DPN rats, and TLN could reduce the demyelination of the sciatic nerve in DPN rats. Metabolomics analysis showed that 14 potential biomarkers (citrate, creatine, fumarate, glyceric acid, glycine, succinate, etc.) of both DPN and TLN treatment were identified. Pathway analysis showed that the changes in these metabolites were mainly related to the citrate cycle (TCA cycle); glycine, serine, and threonine metabolism; and glyoxylate and dicarboxylate metabolism.

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Medicinal plants and phytochemicals with anti-obesogenic potentials: A review

Mopuri

Islam

2017

Biomedicine & Pharmacotherapy

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Mechanism of Tang Luo Ning effect on attenuating of oxidative stress in sciatic nerve of STZ-induced diabetic rats

Cited by 25 publications

References 39 publications

Tangluoning, a traditional Chinese medicine, attenuates in vivo and in vitro diabetic peripheral neuropathy through modulation of PERK/Nrf2 pathway

Tangluoning, a traditional Chinese medicine, attenuates in vivo and in vitro diabetic peripheral neuropathy through modulation of PERK/Nrf2 pathway

Effects of Tang Luo Ning on diabetic peripheral neuropathy in rats revealed by LC–MS metabolomics approach

Medicinal plants and phytochemicals with anti-obesogenic potentials: A review

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