Mechanism of rat osteosarcoma cell apoptosis induced by a combination of low-intensity ultrasound and 5-aminolevulinic acid in vitro

Li, Y N; Zhou, Qi; Yang, Bin; Hu, Zheng; Wang, J H; Li, Q S; Cao, Wenwu

doi:10.4238/2015.august.14.23

Cited by 13 publications

(7 citation statements)

References 12 publications

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“…The US-induced secondary metabolism observed in plant cell cultures could be regulated by low-level ROS, which have been shown to be produced in sonicated cell-free solutions [92,93] and in cells upon exposure to moderate U.S. [94]. This effect has become a background for the sonodynamic therapy of tumours [95,96].…”

Section: Redox Regulation Of Secondary Metabolite Synthesis In Plamentioning

confidence: 99%

Meristem Plant Cells as a Sustainable Source of Redox Actives for Skin Rejuvenation

Korkina¹,

Mayer²,

Luca³

2017

Biomolecules

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Recently, aggressive advertisement claimed a “magic role” for plant stem cells in human skin rejuvenation. This review aims to shed light on the scientific background suggesting feasibility of using plant cells as a basis of anti-age cosmetics. When meristem cell cultures obtained from medicinal plants are exposed to appropriate elicitors/stressors (ultraviolet, ultrasound ultraviolet (UV), ultrasonic waves, microbial/insect metabolites, heavy metals, organic toxins, nutrient deprivation, etc.), a protective/adaptive response initiates the biosynthesis of secondary metabolites. Highly bioavailable and biocompatible to human cells, low-molecular weight plant secondary metabolites share structural/functional similarities with human non-protein regulatory hormones, neurotransmitters, pigments, polyamines, amino-/fatty acids. Their redox-regulated biosynthesis triggers in turn plant cell antioxidant and detoxification molecular mechanisms resembling human cell pathways. Easily isolated in relatively large quantities from contaminant-free cell cultures, plant metabolites target skin ageing mechanisms, above all redox imbalance. Perfect modulators of cutaneous oxidative state via direct/indirect antioxidant action, free radical scavenging, UV protection, and transition-metal chelation, they are ideal candidates to restore photochemical/redox/immune/metabolic barriers, gradually deteriorating in the ageing skin. The industrial production of plant meristem cell metabolites is toxicologically and ecologically sustainable for fully “biological” anti-age cosmetics.

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Section: Redox Regulation Of Secondary Metabolite Synthesis In Plamentioning

confidence: 99%

Meristem Plant Cells as a Sustainable Source of Redox Actives for Skin Rejuvenation

Korkina¹,

Mayer²,

Luca³

2017

Biomolecules

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“…Left ventricular dysfunction in mice with acute myocardial infarction could be attenuated by LIPUS ( 7 ). Biological effects of reagents and drugs were augmented by LIPUS, such as 5-aminolevulinic acid ( 8 ).…”

Section: Introductionmentioning

confidence: 99%

Low-intensity pulsed ultrasound promotes proliferation and migration of HaCaT keratinocytes through the PI3K/AKT and JNK pathways

Leng

Shang

Gao

et al. 2018

Braz J Med Biol Res

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Although the effects of low-intensity pulsed ultrasound (LIPUS) on diverse cell types have been fully studied, the functional role of LIPUS in keratinocytes remains poorly understood. This study aimed to investigate the effects of LIPUS on proliferation and migration of HaCaT cells as well as the regulatory mechanisms associated with signaling pathways. Human HaCaT cells were exposed or not to LIPUS, and cell proliferation and migration were measured by BrdU incorporation assay and Transwell assay, respectively. Expression of proteins associated with proliferation and migration was evaluated by western blot analysis. Expression of key kinases in the PI3K/AKT and JNK pathways was also evaluated by western blot analysis. Effects of LIPUS on the PI3K/AKT and JNK pathways, and whether LIPUS affected HaCaT cells via these two pathways were finally explored. When the parameter of LIPUS (number of cycles) was set at 300, cell viability was the highest after LIPUS stimulation. We then found that the percentage of BrdU positive cells was enhanced by LIPUS, along with up-regulation of cyclinD1, CDK6, CDK4, and VEGF. LIPUS promoted migration, as well as up-regulation of MMP-2 and MMP-9. Phosphorylation levels of key kinases in the PI3K/AKT and JNK pathways were increased by LIPUS. Inhibition of either PI3K/AKT pathway or JNK pathway attenuated effects of LIPUS on HaCaT cells, and co-inhibition of these two pathways showed augmented effects. LIPUS promoted proliferation and migration of HaCaT cells through activating the PI3K/AKT and JNK pathways.

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“…As observed by some experiments, the tumor inhibitory activity of SDT on tumor cells was mainly to induce cell apoptosis14353940. The pro-apoptotic effect and related bio-mechanisms vary with the type of sonosensitizers used in cancer treatment.…”

Section: Discussionmentioning

confidence: 95%

“…PpIX, a hematorphyrin derivative, was found preferentially accumulated in rapid proliferating cancer cells, and it is sonosensitive18323334. In previous studies, we showed that ALA can be a good sonosensitizer for SDT as it exhibited significant selectivity on tumor tissues and a synergistic inhibition effect with low intensity ultrasound on SAS cells2526273536. In this work, we demonstrated, both in vivo and in vitro , that the combined use of PpIX and LIU can significantly inhibit the proliferation of SAS cells, which acted through the cell cycle arrest and p53-dependent extrinsic apoptosis pathway.…”

Section: Discussionmentioning

confidence: 99%

Antiproliferative and Apoptosis-inducing Effect of exo-Protoporphyrin IX based Sonodynamic Therapy on Human Oral Squamous Cell Carcinoma

Jie

Zhou

et al. 2017

Sci Rep

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Sonodynamic therapy (SDT) is an innovative modality for cancer treatment. But the biological effect of SDT on oral squamous cell carcinoma has not been studied. Our previous study has shown that endo-Protoporphyrin IX based SDT (ALA-SDT) could induce apoptosis in human tongue squamous carcinoma SAS cells through mitochondrial pathway. Herein, we investigated the effect of exo- Protoporphyrin based SDT (PpIX-SDT) on SAS cells in vitro and in vivo. We demonstrated that PpIX-SDT increased the ratio of cells in the G2/M phase and induced 3–4 times more cell apoptosis compared to sonocation alone. PpIX-SDT caused cell membrane damage prior to mitochondria damage and upregulated the expression of Fas and Fas L, while the effect was suppressed if cells were pre-treated with p53 inhibitor. Additionally, we examined the SDT-induced cell apoptosis in two cell lines with different p53 status. The increases of p53 expression and apoptosis rate in wild-type p53 SAS cells were found in the SDT group, while p53-mutated HSC-3 cells did not show such increase. Our data suggest that PpIX-SDT suppress the proliferation of SAS cells via arresting cell cycle at G2/M phase and activating the extrinsic Fas-mediated membrane receptor pathway to induce apoptosis, which is regulated by p53.

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Mechanism of rat osteosarcoma cell apoptosis induced by a combination of low-intensity ultrasound and 5-aminolevulinic acid in vitro

Cited by 13 publications

References 12 publications

Meristem Plant Cells as a Sustainable Source of Redox Actives for Skin Rejuvenation

Meristem Plant Cells as a Sustainable Source of Redox Actives for Skin Rejuvenation

Low-intensity pulsed ultrasound promotes proliferation and migration of HaCaT keratinocytes through the PI3K/AKT and JNK pathways

Antiproliferative and Apoptosis-inducing Effect of exo-Protoporphyrin IX based Sonodynamic Therapy on Human Oral Squamous Cell Carcinoma

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