Mechanism of NF-κB signaling pathway and autophagy in the regulation of osteoblast differentiation

Han, Qinghua; Xu, Hui; Gong, Yong-qing

doi:10.1080/09687688.2017.1400601

Cited by 6 publications

(6 citation statements)

References 30 publications

(27 reference statements)

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“…We previously reported that osteoblasts themselves robustly induce autophagy, and the expression of autophagyrelated proteins was altered significantly by the action of NF-kappa B inhibitor SN50. The results suggested that the autophagy function of osteoblasts during tooth eruption plays an important role through the NF-kappa B signaling pathway (Qin et al, 2016). As an important regulator of osteoblasts, it is important to determine how POSTN relates to the autophagy function of osteoblasts and how it regulates the dynamic balance between osteoblasts and osteoclasts.…”

Section: Figmentioning

confidence: 99%

“…Previous studies have shown that cortical bone density and bone strength have decreased in POSTN-knockout mice, but the specific mechanism is not yet clear (Bonnet et al, 2013). Our previous studies have shown that osteoblasts themselves robustly induce autophagy (Qin et al, 2016). However, the effect of autophagy on osteoblasts during tooth eruption and the association between this effect and tooth eruption are unknown.…”

Section: Introductionmentioning

confidence: 95%

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Effect ofPeriostinSilencing on the Autophagy of Osteoblasts

Han

Cai²

2019

Cellular Reprogramming

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The objective of the present work was to investigate the effect of Periostin (POSTN) silencing on autophagy in osteoblasts, and provide an experimental basis for studying the mechanism of dental eruption. The cells were divided into the following four groups according to their viral number: the NC group, pFU-GW-016PSC53349-1; group KD1, LVpFU-GW-016PSC66471-1; group KD2, LVpFU-GW-016PSC66472-1; and group KD3, LVpFU-GW-016PSC66473-1. The lentiviral vector was infected at MOI = 100 in the ENi.S medium containing 5 g/mL Polybrene. The target gene expression was observed by a Celigo Ò Image Cytometer at 72 hours after infection, and the positive rate of fluorescence was noted. A two-step method of quantitative real-time PCR (qRT-PCR) was used to detect the silencing effect of POSTN. Western blotting was then performed to assess the expression of autophagy-related proteins Beclin-1 and LC3 in the group showing the best gene silencing effects. The experimental results showed that there was strong green fluorescence in group KD3. As confirmed via qRT-PCR analysis, the POSTN silencing efficiency in group KD3 reached 92.1%. The Western blotting revealed that the expression of Beclin-1 protein in group KD3 was significantly higher than that in the NC group. However, the LC3 protein expression was not significantly different from that of the control group. The lentiviral vector targeting POSTN in osteoblasts was constructed successfully. In addition, the expression of autophagy protein in mouse osteoblasts increased after POSTN silencing. This finding may provide new approaches for understanding the molecular signal transduction of POSTN during the tooth eruption process.

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Section: Figmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

Effect ofPeriostinSilencing on the Autophagy of Osteoblasts

Han

Cai²

2019

Cellular Reprogramming

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“…SN50, a blocking agent of the NF-κB pathway, exerts a significant effect on autophagy in osteoblasts only when administered at a certain concentration (25 mg/mL). At this dose, it significantly inhibited autophagic flux in osteoblasts by blocking the NF-κB pathway (Qin et al, 2016). Tnf-α promoted osteoblast autophagy and reduced osteoblast apoptosis in avascular necrotic tissue in the femoral head by activating the P38 MAPK/NF-κB signaling pathway (Zheng et al, 2020).…”

Section: Frontiers Inmentioning

confidence: 99%

Regulatory signaling pathways of osteoblast autophagy in periprosthetic osteolysis

Gu,

Wu,

Xie

et al. 2024

Front. Cell Death

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Periprosthetic osteolysis is a difficult-to-treat complication of arthroplasty. The pathological mechanisms of periprosthetic osteolysis are mainly weakened function of osteoblasts and excessive activation of osteoclasts. Many studies have demonstrated that the imbalance between the formation of bone by osteoblasts and the absorption of bone by osteoclasts is the direct cause of osteolytic diseases. Autophagy, as an important self-protective cellular mechanism, has significant effects on the regulation of osteoblast function, such as osteoblast differentiation, proliferation, and apoptosis. Osteoblasts, which play an important role in maintaining bone homeostasis, have attracted increasing attention in recent years. Up till now, Several signaling pathways have been proved to regulate autophagy of osteoblasts, including the AMPK, NF-κB, FoxO3 and other signaling pathways. This article reviews the recent progress in understanding osteoblast autophagy and mitophagy in the context of periprosthetic osteolysis and the signaling pathways which are involved in these processes. By summarizing previous studies describing the mechanism underlying osteoblast autophagy, we wish to contribute new therapeutic ideas and potential therapeutic targets for periprosthetic osteolysis.

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“…Osteoblasts’ autophagy-related function changes significantly when the NF-κB signaling pathway is disabled, demonstrating a possible connection between the two mechanisms 220 . However, according to some studies, NF-κB appears to play two roles in autophagy.…”

Section: Noncanonical Signaling Pathways In Osteoblast Differentiationmentioning

confidence: 99%

Cell signaling and transcriptional regulation of osteoblast lineage commitment, differentiation, bone formation, and homeostasis

Zhu,

Chen,

Masson

et al. 2024

Cell Discov

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The initiation of osteogenesis primarily occurs as mesenchymal stem cells undergo differentiation into osteoblasts. This differentiation process plays a crucial role in bone formation and homeostasis and is regulated by two intricate processes: cell signal transduction and transcriptional gene expression. Various essential cell signaling pathways, including Wnt, BMP, TGF-β, Hedgehog, PTH, FGF, Ephrin, Notch, Hippo, and Piezo1/2, play a critical role in facilitating osteoblast differentiation, bone formation, and bone homeostasis. Key transcriptional factors in this differentiation process include Runx2, Cbfβ, Runx1, Osterix, ATF4, SATB2, and TAZ/YAP. Furthermore, a diverse array of epigenetic factors also plays critical roles in osteoblast differentiation, bone formation, and homeostasis at the transcriptional level. This review provides an overview of the latest developments and current comprehension concerning the pathways of cell signaling, regulation of hormones, and transcriptional regulation of genes involved in the commitment and differentiation of osteoblast lineage, as well as in bone formation and maintenance of homeostasis. The paper also reviews epigenetic regulation of osteoblast differentiation via mechanisms, such as histone and DNA modifications. Additionally, we summarize the latest developments in osteoblast biology spurred by recent advancements in various modern technologies and bioinformatics. By synthesizing these insights into a comprehensive understanding of osteoblast differentiation, this review provides further clarification of the mechanisms underlying osteoblast lineage commitment, differentiation, and bone formation, and highlights potential new therapeutic applications for the treatment of bone diseases.

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Mechanism of NF-κB signaling pathway and autophagy in the regulation of osteoblast differentiation

Cited by 6 publications

References 30 publications

Effect ofPeriostinSilencing on the Autophagy of Osteoblasts

Effect ofPeriostinSilencing on the Autophagy of Osteoblasts

Regulatory signaling pathways of osteoblast autophagy in periprosthetic osteolysis

Cell signaling and transcriptional regulation of osteoblast lineage commitment, differentiation, bone formation, and homeostasis

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