Mechanism of M-cell differentiation accelerated by proliferation of indigenous bacteria in rat Peyer’s patches

Yuasa, Hideto; Mantani, Youhei; Masuda, Natsumi; Nishida, Miho; Arai, Mamoru; Yokoyama, Toshifumi; Tsuruta, Hiroki; Kawano, Junichi; Hoshi, Nobuhiko; Kitagawa, Hiroshi

doi:10.1292/jvms.17-0470

Cited by 4 publications

(2 citation statements)

References 29 publications

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“…It was shown that in mice specific bacteria determine mucosal IgA production with followed mucosal immune response, they enhance intestinal IgA levels by inducing TGF-β in T follicular helper cells of PP Bacteria belonged to Anaeroplasma are sufficient to induce these changes [9]. In rat's PP the indigenous bacteria accelerate the differentiation of microvillous columnar epithelial cells into M-cells [10]. The growth of bacterial colonies between the intervillous spaces associates with the change of histological localization of the lymphocyte lineage in small intestine mucosa of rats [11].…”

Section: Discussionmentioning

confidence: 99%

Effect of probiotic strains upon intestinal immune system under cold stress

et al. 2020

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Gut microbiome changes can be considered as a causative factor of stress-associated intestinal diseases. The aim of the investigation was to study the effects of the oral probiotic strains Lactobacillus casei LB 148 and E. coli EB 387 onto gut microbiota, as well as the intestinal immune system of rats Sprague Dawley in a healthy state and under cold stress. There are no strict official recommendations here in the Russian Federation for preclinical studies of new probiotic strains. Here we describe a method used to estimate the safety and effectiveness of the oral use of a probiotic. It is cheap and simple, so we recommend using it at least for the screening search of effective and safe probiotics. The number of Peyer’s patches in the small intestine of rats in different compartments was counted and then compared between different groups of rats. After cold stress there was a change in the number of Peyer’s patches together with microbiota shifts. Lactobacillus casei LB 148 as well as E. coli EB 387 protected the immune system against the stress effects.

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Section: Discussionmentioning

confidence: 99%

Effect of probiotic strains upon intestinal immune system under cold stress

et al. 2020

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“…After euthanasia by overdose peritoneal injection of pentobarbital sodium (Kyoritsu Seiyaku Corp., Tokyo, Japan), tissue blocks were removed from the ileum including the Peyer’s patches. Then, the tissue blocks were immersion-fixed in 4.0% paraformaldehyde fixative in 0.1 M phosphate buffer for 6 hr at 4°C, and snap-frozen in liquid nitrogen as described in a previous study [47]. Four micrometer-thick sections were cut using a Coldtome Leica CM1950 (Leica Biosystems, Nussloch, Germany), placed on slide glasses precoated with 0.2% 3-aminopropyltriethoxysilane (Shin-Etsu Chemical Co., Tokyo, Japan), and stored at −30°C until use.…”

Section: Methodsmentioning

confidence: 99%

Effects of the expansion of bacterial colonies into the intervillous spaces on the localization of several lymphocyte lineages in the rat ileum

Yuasa

Mantani

Miyamoto

et al. 2019

The Journal of Veterinary Medical Science

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The effect of bacterial colonies expanded into the intervillous spaces on the localization of several lymphocyte lineages was immunohistochemically investigated in two types of mucosa: ordinary mucosa of rat ileum, which consists of mucosa without any mucosal lymphatic tissue; and follicle-associated mucosa (FAM), which accompanies the parafollicular area under the muscularis mucosae in the rat ileal Peyer’s patch. The results showed that bacterial colonies in the intervillous spaces induced increased populations of CD8 + cells in the epithelium of the intestinal villus in ordinary mucosa (IV) and intestinal villus in FAM (IV-FAM). Bacterial colonies in the intervillous spaces were also associated with increased numbers of IgA + cells, which were mainly localized in the lamina propria of basal portions of IV and IV-FAM, and with expanded localization of IgA + cells into the villous apex in both IV and IV-FAM. Moreover, IgA + cells around the intestinal crypts adjacent to IV or IV-FAM were also increased in response to bacterial colonies. In the IV-FAM, but not IV, L-selectin + cells, which were found to be immunopositive for TCRαβ or CD19, were drastically increased in the lamina propria from the crypt to middle portion of IV-FAM and in the lumen of central lymph vessel of IV-FAM in response to the bacterial colonies in the intervillous spaces. These findings revealed that the expansion of bacterial colonies into the intervillous spaces accompanies the change of histological localization of the lymphocyte lineage in both the ordinary mucosa and FAM.

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