MECHANISM OF INACTIVATION OF Α-Estradiol BY RAT LIVER “IN VITRO”

Demeio, R. H.; Rakoff, A. E.; Cantarow, A.; Paschkis, J. E.

doi:10.1097/00006254-194904000-00048

Cited by 2 publications

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“…Liver mince is not as effective as slices in performing this inactivation of a-estradiol, suggesting that the enzyme system involved is dependent upon cellular integrity. It has recently been demonstrated (DeMeio et al (48), Coppedge et al (49,50)) that nicotinamide and diphosphopyridine nucleotide (cozymase) enhance the activity of liver mince. Israel, Meranze and Johnston (51) added estrone to the perfusate of a liver, heart-lung preparation and obtained inactivation of the estrogen.…”

Section: The Role Of the Liver In The Inactivation Of Estrogensmentioning

confidence: 99%

The Metabolism of the Estrogens: A Review

Jailer

1949

The Journal of Clinical Endocrinology & Metabolism

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Section: The Role Of the Liver In The Inactivation Of Estrogensmentioning

confidence: 99%

The Metabolism of the Estrogens: A Review

Jailer

1949

The Journal of Clinical Endocrinology & Metabolism

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“…Twombly and Taylor (5) showed that human liver inactivates 17-pt estradiol less than does rat liver. It is generally accepted that liver mince is not as effective as slices in inactivating estradiol but De Meio, Rakoff, Cantarow and Paschkis (6), and Coppedge, Segaloff, Sarett and Altschul (7,8) have shown that nicotinamide or diphosphorpyridine nucleotide enhance the activity of liver mince.…”

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confidence: 99%

The in Vitro Metabolism of Estrogens by the Liver Studied by Means of a Colorimetric Method 12

Lieberman¹,

Tagnon²,

Schulman³

1952

J. Clin. Invest.

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Since the work of Zondek (1) the in vitro action of liver slices and liver pulp on natural estrogens is usually called inactivation because the transformation undergone by these compounds results in loss of estrogenic activity as measured by bioassay methods (2). Using cc. of toluene were added to the mixture. All these operations were carried out in an ice bath.5 The colored material was extracted into toluene by shaking for 10 minutes in a shaking machine. After centrifuging for two minutes at 2,500 r.p.m., the toluene layer was pipetted off and read in a Beckman quartz spectrophotometer (Model DU) at 425 m#* in a 1 cm. cell against the blank.The blank was treated as the experimental sample except that no estrogen was present.Quantitative measurements of 17-j estradiol, estrone and estriol were obtained by this procedure between 10 and 150 micrograms, as indicated on Figure 1. The relation between optical densities and concentrations followed Beer's law between these two values. The slope of the curve was found to be similar for estrone and estradiol but different for estriol (Figure 1).The absorption spectrum of the colored compound showed a plateau-like peak between 400 mAi' and 450 miA with the maximum at 425 my approximately (Figure 2). 4 Kindly supplied by Drs. E. Schwenk and E. B. Hirshbirg, Schering Corporation, Bloomfield, New Jersey. 5 The tetrazotized dianisidine solution was prepared as follows: 25 mg. of dianisidine dihydrochloride (recrystallized from ethanol) were dissolved in 3 cc. of water. Three tenths cc. of concentrated hydrochloric acid, followed by 0.6 cc. of freshly prepared 5% sodium nitrite solution were added. After thorough mixing and a five minute waiting period, 0.66 cc. of a 5% urea solution was added. The solution was prepared in an ice bath, and made freshly for each experiment.341

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MECHANISM OF INACTIVATION OF Α-Estradiol BY RAT LIVER “IN VITRO”

Cited by 2 publications

References 0 publications

The Metabolism of the Estrogens: A Review

The Metabolism of the Estrogens: A Review

The in Vitro Metabolism of Estrogens by the Liver Studied by Means of a Colorimetric Method 12

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