Mechanism of inactivation of influenza viruses by immobilized hydrophobic polycations

Hsu, Bryan B.; Wong, Sze Yinn; Hammond, Paula T.; Chen, Jianzhu; Klibanov, Alexander M.

doi:10.1073/pnas.1017012108

Cited by 95 publications

(103 citation statements)

References 38 publications

(45 reference statements)

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“…As seen in Table 4, regardless of the mode of coating, the resultant surfaces were completely lethal to the human Wuhan strain of influenza virus. Presumably, the hydrophobic Quat-12-PU coatings disrupt the lipid envelope of the virus protecting its RNA, as was previously demonstrated for N,N-dodecyl,methyl-PEI coatings [16]. However, Quat-12-PU-coated surfaces failed to inactivate poliovirus (Table 4).…”

Section: Determination Of Coating Cycle By Charge Density Of Quaternamentioning

confidence: 59%

“…Viruses were obtained from the U.S. Centers for Disease Control and Prevention (CDC) (Wuhan strain of influenza) and the American Type Tissue Collection (ATCC) (Chat strain of poliovirus). Madin-Darby canine kidney (MDCK) and HeLa cells were purchased from ATCC and maintained as previously reported [16,17]. Escherichia coli and Staphylococcus aureus bacteria were purchased from ATCC (catalog numbers 6538 and 15597, respectively) and grown in LB broth.…”

Section: Methodsmentioning

confidence: 99%

“…In addition, such polyQACs as N,N-dodecyl,methyl-PEI have been recently reported to inactivate enveloped viruses, including both human and avian strains of influenza virus [15,16], and disinfect solutions containing non-enveloped poliovirus or rotavirus [17].…”

mentioning

confidence: 99%

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Antiviral and Antibacterial Polyurethanes of Various Modalities

Park

Larson

Klibanov

et al. 2013

Appl Biochem Biotechnol

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We have prepared and characterized a new polyurethane-based antimicrobial material, N,N-dodecyl,methyl-polyurethane (Quat-12-PU). It exhibits strong antiviral and antibacterial activities when coated (as an organic solution or an aqueous nanosuspension) onto surfaces and antibacterial activity when electrospun into nanofibers. Quat-12-PU surfaces are able to kill airborne Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli bacteria, as well as inactivate the enveloped influenza virus (but not the non-enveloped poliovirus).

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Section: Determination Of Coating Cycle By Charge Density Of Quaternamentioning

confidence: 59%

Section: Methodsmentioning

confidence: 99%

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Antiviral and Antibacterial Polyurethanes of Various Modalities

Park

Larson

Klibanov

et al. 2013

Appl Biochem Biotechnol

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“…17 In all cases, the mechanism of disruption was ascribed to hydrophobic interactions. However, a systematic study that examines this mechanism as a function of other physicochemical properties of the surface in addition to hydrophobicity is lacking.…”

Section: 14-16mentioning

confidence: 99%

Functionalized Surfaces with Tailored Wettability Determine Influenza A Infectivity

Mannelli

Reigada²,

Suarez

et al. 2016

ACS Appl. Mater. Interfaces

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Surfaces contaminated with pathogenic microorganisms contribute to their transmission and spreading. The development of "active surfaces" that can reduce or eliminate this contamination necessitates a detailed understanding of the molecular mechanisms of interactions between the surfaces and the microorganisms. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 shown that, among the different surface characteristics, the wetting properties play an important role in reducing virus infectivity. Here, we systematically tailored the wetting characteristics of flat and nanostructured glass surfaces by functionalizing them with alkyl-and fluoro-silanes. We studied the effects of these functionalized surfaces on the infectivity of Influenza A viruses using a number of experimental and computational methods including real-time fluorescence microscopy and molecular dynamics simulations. Overall, we show that surfaces that are simultaneously hydrophobic and oleophilic are more efficient in deactivating enveloped viruses. Our results suggest that the deactivation mechanism likely involves disruption of the viral membrane upon its contact with the alkyl chains. Moreover, enhancing these specific wetting characteristics by surface nanostructuring led to an increased deactivation of viruses. These combined features make these substrates highly promising for applications in hospitals and similar infrastructures where antiviral surfaces can be crucial.

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“…While most work on flowering and the CO–FT regulon has centered on annual herbaceous taxa, a pair of studies have examined the recently derived paralogs PtFT 1 and PtFT 2, FT homologs in the long-lived tree Populus trichocarpa (Salicaceae; Bohlenius et al, 2006; Hsu et al, 2011). Several lines of evidence indicate that PtFT 1 promotes floral initiation.…”

Section: Emerging Dicot Models: Evidence For Diversity In Ft Homolog mentioning

confidence: 99%

In the Light of Evolution: A Reevaluation of Conservation in the CO?FT Regulon and Its Role in Photoperiodic Regulation of Flowering Time

Ballerini¹,

Kramer²

2011

Front. Plant Sci.

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In order to maximize reproductive success, plants have evolved different strategies to control the critical developmental shift marked by the transition to flowering. As plants have adapted to diverse environments across the globe, these strategies have evolved to recognize and respond to local seasonal cues through the induction of specific downstream genetic pathways, thereby ensuring that the floral transition occurs in favorable conditions. Determining the genetic factors involved in controlling the floral transition in many species is key to understanding how this trait has evolved. Striking genetic discoveries in Arabidopsis thaliana (Arabidopsis) and Oryza sativa (rice) revealed that similar genes in both species control flowering in response to photoperiod, suggesting that this genetic module could be conserved between distantly related angiosperms. However, as we have gained a better understanding of the complex evolution of these genes and their functions in other species, another possibility must be considered: that the genetic module controlling flowering in response to photoperiod is the result of convergence rather than conservation. In this review, we show that while data clearly support a central role of FLOWERING LOCUS T (FT) homologs in floral promotion across a diverse group of angiosperms, there is little evidence for a conserved role of CONSTANS (CO) homologs in the regulation of these loci. In addition, although there is an element of conserved function for FT homologs, even this component has surprising complexity in its regulation and evolution.

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Mechanism of inactivation of influenza viruses by immobilized hydrophobic polycations

Cited by 95 publications

References 38 publications

Antiviral and Antibacterial Polyurethanes of Various Modalities

Antiviral and Antibacterial Polyurethanes of Various Modalities

Functionalized Surfaces with Tailored Wettability Determine Influenza A Infectivity

In the Light of Evolution: A Reevaluation of Conservation in the CO?FT Regulon and Its Role in Photoperiodic Regulation of Flowering Time

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