[Retracted] Mechanism of Gegen Qinlian Decoction Regulating ABTB1 Expression in Colorectal Cancer Metastasis Based on PI3K/AKT/FOXO1 Pathway

Abstract

It was to investigate the role of Gegen Qinlian decoction (GQD) in the regulation of ABTB1 gene based on PI3K/AKT/FOXO1 signaling pathway in colorectal cancer (CRC) metastasis. In this study, 10 cases of the CRC mouse model were established by inoculating CT26 cells into the spleen of mice, which were divided into the experimental group and the control group, 5 cases in each group; the control group was intragastrically administered with normal saline 0.3 mL/d, and the experimental group was intragastrically administered with GQD 0.2 mL/d at a ratio of 0.2 g medicinal materials/10 g for 10 days and sacrificed, and pathological sections were made. The expression density of signaling pathway PI3K/AKT/FOXO1 as well as gene ABTB1 was detected in the sections of the two groups, and the mechanism of action of this gene in the two groups of mice was studied. It was found that the densities of p-PI3K, p-AKT, and p-FOXO1 in the experimental group of mice were 26.55 g/cm3, 70.2 g/cm3, and 24.36 g/cm3, respectively, which were significantly increased compared with the control group, P < 0.05 ; the density of ABTB1 was 35.4 g/cm3, which was significantly increased compared with the control group, P < 0.05 ; the proliferation and migration ability of CRC cells in the experimental group were significantly decreased, P < 0.05 . GQD can promote the expression of ABTB1 by activating the PI3K/AKT/FOXO1 signaling pathway, in order to inhibit the proliferation and growth ability of CRC cells.

Multi-Omics Analysis of MCM2 as a Promising Biomarker in Pan-Cancer

“…We also investigated the gene expression characteristics of MCM2 which presented a high mutation rate, indicating that MCM2 may be a potential marker for diagnosing various cancers. We found the top-10 gene that was co-mutated with MCM2, which have been reported in multiple cancers, such as colorectal cancer ( Li et al, 2022 ), BRCA ( Lin et al, 2020 ), LUAD ( Zhang et al, 2020 ) and LIHC ( Li et al, 2021a ). Furthermore, we integrated MCM2 in the GGI and PPI networks and found that it was strongly associated with genes and proteins related to the cell cycle and DNA replication, such as the CDC family, a group of genes coding proteases and phosphatases that regulate the cell division phase and cell cycle, and POLA1, a protein contributing to the DNA replication pathway.…”

Traditional Chinese medicine for colorectal cancer treatment: potential targets and mechanisms of action

“…), Huang Qin ( Scutellaria baicalensis Georgi), Huang Lian ( Coptis chinensis Franch.) – CT-26 spleen transplanted mice Proliferation ↓, Migration ↓ ↑: p-PI3K, p-AKT, p-FOXO1, ABTB1 PI3K/AKT/FOXO1 [ 55 ] Tounong Powder extracts (TNSEs) Huang Qi ( Astragalus membranaceus (Fisch. )), Dang Gui ( Aaugellica sinensis (Oliv) Diels.…”

“…Li et al [ 55 ] found that Gegen Qinlian Decoction (GQD) could inhibit CT-26 CRC growth accompanied by upregulation of p-PI3K, p-Akt, phosphorylated forkhead box transcription factor O1 (p-FOXO1), and ankyrin repeat and BTB/POZ domain containing protein 1 (ABTB1). Fang et al [ 56 ] found that Tounong powder extracts (TNSEs) induced LoVo cell growth inhibition in a dose- and time-dependent manner; significantly downregulated the expression of PI3K, p-AKT, phosphorylated mechanistic target of rapamycin (p-mTOR), and p-p70s6k1; and upregulated the expression of cleaved caspase-9 and -3.…”

Retracted: Mechanism of Gegen Qinlian Decoction Regulating ABTB1 Expression in Colorectal Cancer Metastasis Based on PI3K/AKT/FOXO1 Pathway

“…This article has been retracted by Hindawi following an investigation undertaken by the publisher [ 1 ]. This investigation has uncovered evidence of one or more of the following indicators of systematic manipulation of the publication process: Discrepancies in scope Discrepancies in the description of the research reported Discrepancies between the availability of data and the research described Inappropriate citations Incoherent, meaningless and/or irrelevant content included in the article Manipulated or compromised peer review …”

“…This article has been retracted by Hindawi following an investigation undertaken by the publisher [1]. This investigation has uncovered evidence of one or more of the following indicators of systematic manipulation of the publication process:…”