Mechanism of Formation and Construction of Self-Assembled Myoglobin/Hyaluronic Acid Multilayer Films: An Electrochemical QCM, Impedance, and AFM Study

Pinto, Edilson M.; Bârsan, Mădălina M.; Brett, Christopher M.A.

doi:10.1021/jp107107b

Cited by 21 publications

(14 citation statements)

References 33 publications

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“…The significant advantage of LBL self-assembly method is the number of layers and film thickness are precisely controlled. In addition, this method requires a small quantity of materials [ 32 , 33 ]. The fabrication of PANi-AuNPs composite multilayers through the LBL method was studied for simultaneous detection of DA and UA.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Dopamine Biosensor Based on Poly(3-aminobenzylamine) Layer-by-Layer Self-Assembled Multilayer Thin Film

2021

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Dopamine (DA) is an important neurotransmitter which indicates the risk of several neurological diseases. The selective determination with low detection limit is necessary for early diagnosis and prevention of neurological diseases associated with abnormal concentration of DA. The purpose of this study is to fabricate a poly(3-aminobenzylamine)/poly(sodium 4-styrenesulfonate) (PABA/PSS) multilayer thin film for use as an electrochemical DA biosensor. The PABA was firstly synthesized using a chemical oxidation method of 3-aminobenzylamine (ABA) monomer with ammonium persulfate (APS) as an oxidant. For electrochemical biosensor, the PABA/PSS thin film was fabricated on fluorine doped tin oxide (FTO)-coated glass substrate using the layer-by-layer (LBL) self-assembly method. The optimized number of bilayers was achieved using SEM and cyclic voltammetry (CV) results. The electroactivity of the optimized LBL thin film toward detection of DA in neutral solution was studied by CV and amperometry. The PABA/PSS thin film showed good sensitivity for DA sensing with sensitivity of 6.922 nA.cm−2.µM−1 and linear range of 0.1–1.0 µM (R2 = 0.9934), with low detection limit of 0.0628 µM, long-term stability and good reproducibility. In addition, the selectivity of the PABA/PSS thin film for detection of DA under the common interferences (i.e., ascorbic acid, uric acid and glucose) was also presented. The prepared PABA/PSS thin film showed the powerful efficiency for future use as DA biosensor in real sample analysis.

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Section: Introductionmentioning

confidence: 99%

Electrochemical Dopamine Biosensor Based on Poly(3-aminobenzylamine) Layer-by-Layer Self-Assembled Multilayer Thin Film

2021

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“…Modern QCM‐devices can detect sub‐monolayer amounts of adsorbates and provide a stable signal under high loadings . The QCM method combined with dissipation measurements (QCM‐D) are often employed to characterize protein monolayers . The method provides the surface coverage of the adsorbed molecules, including the associated electrolyte molecules.…”

Section: Introductionmentioning

confidence: 99%

“…It is in many cases absolutely necessary that low‐ and ultra‐low volume cells are used to minimize the amount of scarce materials. For example, the assembly of colloidal redox‐active particles or multilayers of redox proteins can be followed using the increase in mass and charge obtained in low volume CV‐EQCM systems. However, the performance of low volume devices is in many cases not sufficient for accurate characterization of monolayer or sub‐monolayer amounts of nano‐objects such as proteins or nanoparticles during assembly.…”

Section: Introductionmentioning

confidence: 99%

Anodic Desorption Monitored by Voltammetric and Gravimetric Measurements for Fast Estimation of Surface Coverage of Complex Organic Molecules on Au Electrodes

2016

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1IntroductionComplex molecular nanostructures and biomolecules assembled att he surface of functional electrodes are key objectsi nr apidly growing areas of electro-catalysis, where reactants are selectively oxidizedo rr educed at the electrode surface,i nt he development of efficient (bio)sensors or (bio)-fuel cells,f or example.In depth investigation of catalyticp roperties,m olecular interactions or other phenomena in nano-objects immobilized at the electrodes urface require precise information about the surface coverage.F or "hard"i norganicp articles,avariety of powerful techniques including, transmission electron microscopy (TEM), scanning electron microscopy (SEM), scanning tunneling microscopy (STM), or atomic force microscopy( AFM) allows direct visualization of the formed structures and straightforward determination of the surface concentrations.W ith "softer" organic or biological nano-objects such techniques can still be useful, especially for imagingo rdered superstructures. However, with dense monolayers (and/or disordered layers) or highlyf lexible structures[ 1],t he accuracyo f surface coverage determination by means of these commonm ethodsi soften very limited.If the molecular nanostructures are electroactive,t he electrochemical techniqueo fp otentiodynamic cyclic voltammetry (CV) can be used to control their assembly and characterizet he resultant layers in as traightforward manner. This technique uses ac yclic linear change in electrode potential asaprobing signal and analyses the direct current flowing through the system. This provides informationa bout the number of electroactivec entres [2][3][4].H owever, the redox centersi naccessible to the electront ransfer will not be detected.A lternatively,f or electrocatylic centers,s uch as redoxe nzymes,m ediatede lectront ransfer may be exploited to quantify the catalytic properties whichi ndirectly provide information on the surface coverageo ft he system.F or this purpose the catalytica ctivity is assumed to be equal to that of the freely diffusing enzyme [5,6].I na ddition, two robust and wellestablished techniques can be usedt oc omplement electrochemical methods and determine surface coverages for non-redox active monolayers [7].T hese techniques are in-situ gravimetry using aq uartz crystalm icrobalance (QCM) [8,9] and surface plasmon resonance (SPR). The SPR methodp rovides the dry weight of the modifiers and has become one of the standard methods to investigate protein adsorption [10].H owever, interpretation of the data withr espect to surface mass requires preliminary calibrations and assumptions to be made [11].Abstract:Am ethodology for fast determinationo fs urface coverage of molecules or nanostructures assembled in monolayers at the surface of gold electrodes has been developed. Thea pproach is based on anodic oxidative desorptiono ft he molecules which is controlled by synchronized electrochemical gravimetric and voltammetric measurements.S uccessivea nodic scans induce degradation and oxidative desorption of the assembled molecu...

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“…In addition, electrochemical impedance spectroscopy (EIS) was used to investigate the electrical properties and electron transfer [27][28][29] resulting from Ni-Salpn/substrate and/or Ni-Salpn/solution interface interactions. Characterization of film growth was carried out by the electrochemical quartz crystal microbalance (EQCM) and UV-vis absorption spectroscopy.…”

Section: Introductionmentioning

confidence: 99%

Nickel- N,N’ – bis (salicylidene)-1,3-propanediamine (Ni- Salpn ) film-modified electrodes. Influence of electrodeposition conditions and of electrode material on electrochemical behaviour in aqueous solution

Martin

Gouveia‐Caridade

Crespilho

et al. 2015

Electrochimica Acta

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Influence of electrode material EQCM UV-vis and Raman spectroscopy a b s t r a c t Modified electrodes based on films of Schiff base complexes are excellent candidates for sensing applications. The influence of the electrode material, glassy carbon, platinum, gold or indium tin oxide, on the electrodeposition of nickel-N,N'-bis(salicylidene)-1,3-propanediamine (Ni-Salpn) films in 1,2-dichloroethane (DCE) was investigated, and their electrochemical behaviour was evaluated by cyclic voltammetry and electrochemical impedance spectroscopy. The effect of the electrodeposition potential and electrodeposition time on the electrochemical behaviour of Ni-Salpn was examined using glassy carbon as substrate. The film growth process was investigated using the electrochemical quartz crystal microbalance and UV-vis absorption spectroscopy and structural differences between the Ni-Salpn complex and the Ni-Salpn film were examined by micro-Raman spectroscopy. The results demonstrate that the electrodeposition mechanism is independent of the electrode material, but that the nature of the substrate material influences the rate of film growth, the electrochemical behaviour and the stability of the film-modified electrodes in aqueous solution. Counteranion insertion during oxidation can break the bonds between the complexes in the stacked Ni-Salpn film structure and cause both mass loss and blockage of the redox metal centre activity, but these effects are small in thin films.

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Mechanism of Formation and Construction of Self-Assembled Myoglobin/Hyaluronic Acid Multilayer Films: An Electrochemical QCM, Impedance, and AFM Study

Cited by 21 publications

References 33 publications

Electrochemical Dopamine Biosensor Based on Poly(3-aminobenzylamine) Layer-by-Layer Self-Assembled Multilayer Thin Film

Electrochemical Dopamine Biosensor Based on Poly(3-aminobenzylamine) Layer-by-Layer Self-Assembled Multilayer Thin Film

Anodic Desorption Monitored by Voltammetric and Gravimetric Measurements for Fast Estimation of Surface Coverage of Complex Organic Molecules on Au Electrodes

Nickel- N,N’ – bis (salicylidene)-1,3-propanediamine (Ni- Salpn ) film-modified electrodes. Influence of electrodeposition conditions and of electrode material on electrochemical behaviour in aqueous solution

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