Mechanism of Flavonoids Action in Smooth Muscle Relaxation

Basir, Seemi Farhat

doi:10.20959/wjpps20179-9992

Cited by 3 publications

(1 citation statement)

References 83 publications

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“…The same suggestion of blocking calcium influx through the Ca V was observed with Arrabidaea chica in the smooth muscle of arteries (Cartagenes et al, 2014). In addition, the effect reported for A. chica and now A. brachypoda may be due to the flavonoids present in the phytochemical composition of these two species (Takemura et al, 1995;Alcerito et al, 2002;Siraichi et al, 2013), since the literature shows some plants with flavonoids promoting the blockage of Ca 2+ influx by Ca V (Ghayur et al, 2006;Chen et al, 2009;Carvalho Correia et al, 2013;Basir, 2017;Patel & Patel, 2017).…”

Section: Discussionmentioning

confidence: 98%

Hydroalcoholic extract of leaves of Arrabidaea brachypoda (DC.) Bureau present antispasmodic activity mediated through calcium influx blockage

Monteiro

Costa

Martins³

et al. 2020

Rev. Ciênc. Farm. Básica Apl.

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Aim: Since other species of the Bignoniaceae Family presented of antispasmodic activity, it was decided, by chemotaxonomic criterion, to determine the antispasmodic activity of the leaves of Arrabidaea brachypoda. Methodology: the segments of the rat jejunum were suspended in glass vats containing specific saline solution, at an appropriate temperature, and after stabilization period, were stimulated by a contractile agent to observe the inhibitory or relaxing effect of EH-FAB. Results: EH-FAB showed the presence of 10 compounds, mainly rutin and it has an antispasmodic activity as it inhibits the phasic component and relaxes the tonic component of the contraction in isolated rat jejunum. To assess the mechanism of antispasmodic action, cumulative curves to the CCh were performed in which a non-competitive antagonism was observed, due to a displacement of the control curve to the right and reduction of the maximum contraction effect (E max). Afterward, the participation of the calcium and/or potassium channels was evaluated by increasing the extracellular potassium, and it was observed that the EH-FAB relaxed the rat jejunum, suggesting the participation of the Ca 2+ channels. To corroborate that hypothesis, the EH-FAB was tested against cumulative curves to Ca 2+ in a free depolarizing solution of Ca 2+ , and it was observed that there was a shift of the curve to the right with a reduction in E max. Conclusions: EH-FAB presents antispasmodic activity in isolated rat jejunum and it is suggested to block the influx of Ca 2+ through voltage-gated calcium channels, signaling the therapeutic potential for the treatment of colic and/or diarrhea.

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Section: Discussionmentioning

confidence: 98%

Hydroalcoholic extract of leaves of Arrabidaea brachypoda (DC.) Bureau present antispasmodic activity mediated through calcium influx blockage

Monteiro

Costa

Martins³

et al. 2020

Rev. Ciênc. Farm. Básica Apl.

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Quercetin and resveratrol ameliorate nickel-mediated hypercontraction in isolated Wistar rat aorta

Wani

Khan

Basir

2022

J. Smooth Muscle Res.

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Purpose:The ameliorative potential of quercetin and resveratrol on isolated endothelium-intact aortic rings incubated with nickel was examined. Method:The effect of varying concentrations of quercetin and resveratrol was investigated on isolated Wistar rat aortic rings using an organ bath system over vasoconstrictor phenylephrine (PE) at 1 µM. To delineate the mechanism of action, isolated aortic rings were pre-incubated with pharmacological modulators, such as verapamil 1 µM, apocynin 100 µM, indomethacin 100 µM or N-G-nitro-L-arginine methyl ester (L-NAME) 100 µM, separately, before incubation with 100 µM quercetin and 30 µM resveratrol. To assess the ameliorative and prophylactic potentials of quercetin and resveratrol, aortic rings were also incubated with quercetin or resveratrol for 40 min, followed by incubation with nickel for 40 min.Results: At 100 µM, quercetin caused 29% inhibition of contraction, while resveratrol at 30 µM caused 55% inhibition of contraction in aortic rings compared with control. Aortic rings incubated with contractile modulators, such as verapamil, apocynin, indomethacin or N-G-nitro-L-arginine methyl ester (L-NAME), along with quercetin or resveratrol at their concentrations producing maximum relaxant effect, showed that both of these natural compounds exert their relaxant effect by inhibiting the generation of reactive oxygen species (ROS) from endothelial and smooth muscle cells, blocking voltage-gated calcium channels, and increasing the release of nitric oxide (NO). The mediation of hypercontraction by nickel is due to the increased ROS and the influx of calcium through voltage-dependent calcium channels. These natural compounds are shown to counter the nickel-induced effects, appearing as effective ameliorators. Conclusion:In this study, we found that quercetin and resveratrol act as ameliorators of nickel-mediated hypercontraction by decreasing ROS and enhancing NO release from endothelial cells.

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Potential Benefits of Flavonoids on the Progression of Atherosclerosis by Their Effect on Vascular Smooth Muscle Excitability

2021

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Flavonoids are a group of secondary metabolites derived from plant-based foods, and they offer many health benefits in different stages of several diseases. This review will focus on their effects on ion channels expressed in vascular smooth muscle during atherosclerosis. Since ion channels can be regulated by redox potential, it is expected that during the onset of oxidative stress-related diseases, ion channels present changes in their conductive activity, impacting the progression of the disease. A typical oxidative stress-related condition is atherosclerosis, which involves the dysfunction of vascular smooth muscle. We aim to present the state of the art on how redox potential affects vascular smooth muscle ion channel function and summarize if the benefits observed in this disease by using flavonoids involve restoring the ion channel activity.

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Mechanism of Flavonoids Action in Smooth Muscle Relaxation

Cited by 3 publications

References 83 publications

Hydroalcoholic extract of leaves of Arrabidaea brachypoda (DC.) Bureau present antispasmodic activity mediated through calcium influx blockage

Hydroalcoholic extract of leaves of Arrabidaea brachypoda (DC.) Bureau present antispasmodic activity mediated through calcium influx blockage

Quercetin and resveratrol ameliorate nickel-mediated hypercontraction in isolated Wistar rat aorta

Potential Benefits of Flavonoids on the Progression of Atherosclerosis by Their Effect on Vascular Smooth Muscle Excitability

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