Mechanism of Experimental Sterility Induced in Guinea Pigs by Injection of Homologous Testis and Sperm

Ashitaka, Yoshio; Isojima, Shinzo; Ukita, Hisashi

doi:10.1016/s0015-0282(16)35170-6

Cited by 13 publications

(2 citation statements)

References 3 publications

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“…In animal studies, Edwards could detect only a weak antibody activity in the uterus and oviduct after systemic immunization in the rabbits 32 . However, Ashitaka and Isojima et al could find a strong sperm‐immobilizing activity in the vaginal secretions immunized with homologous sperm or testis in female guinea pigs 33 …”

Section: Reproductive Failures By Anti‐sperm Antibodiesmentioning

confidence: 98%

Anti‐sperm antibodies and reproductive failures

Shibahara

Wakimoto

Fukui

et al. 2020

American J Rep Immunol

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Reproductive failures include infertility and recurrent pregnancy loss (RPL). Although the relative importance of immunological factors in human reproduction remains unclear, there may be immune‐mediated reproductive failures in a portion of unexplained infertility and RPL. As a cause of immunological factor, anti‐sperm antibodies (ASA) are produced both in men and women. There have been reported several antigens in the surface of sperm that are especially foreign to women. The presence of ASA, especially sperm‐immobilizing antibodies, in the sera of infertile women has been shown to inhibit sperm migration in the female genital tract. Therefore, the effectiveness of the treatments for infertile women with sperm‐immobilizing antibodies by timed intercourse or intra‐uterine insemination is limited. Such antibodies can also exert inhibitory effects on various stages of sperm‐egg interaction and subsequent embryo development in vitro. It is suggested that ASA testing for infertile women should be performed before proceeding IVF. The manipulation of gametes and embryos from patients having sperm‐immobilizing antibodies should be carefully carried out especially to avoid contaminating patient's serum and follicular fluid in the culture medium in order to overcome the immunological causes of female infertility by ASA, and satisfactory results under suitable conditions for gametes and embryos have been obtained. The relationship between ASA and RPL was controversially reported. Increased miscarriage rates in women with ASA were demonstrated by some authors. In contrast, lack of association between ASA and RPL was reported. In this manuscript, we are focusing the roles of ASA in women with reproductive failures.

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Section: Reproductive Failures By Anti‐sperm Antibodiesmentioning

confidence: 98%

Anti‐sperm antibodies and reproductive failures

Shibahara

Wakimoto

Fukui

et al. 2020

American J Rep Immunol

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show abstract

“…Sperm agglutination tests were carried out using the microscopic technique of Kiddy et al (1959) and the macroscopic agglutinationin-gel reaction of Kilbrick, Balding & Merrill (1952). In the sperm immobiliza¬ tion test (Ashitaka, Isojima & Ukita, 1964), human serum was used as a source of complement. Negative sera and saline were used as controls in all tests.…”

Section: Methodsmentioning

confidence: 99%

An Immune Response to Egg-Yolk Semen Diluent in Dairy Cows

Griffin¹,

Nunn²,

Hartigan³

1971

Reproduction

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Antibodies to egg-yolk semen diluent antigens were isolated from the majority of samples from the vagina, uterus and serum of cows which had received repeated artificial inseminations with semen diluted in egg-yolk diluent. The vagina was the site from which antibodies were isolated most frequently. There was evidence that the titres of antibodies in the genital tract resulted in part, at least, from local antibody production by the vaginal and uterine tissues.Samples with titres to egg-yolk diluent antigens, when tested in vitro, caused mixed agglutination of homologous spermatozoa diluted in egg\ x=req-\ yolk diluent, while non-specific head-to-head agglutination was seen when the spermatozoa had been diluted in saline. When the cows were inseminated with homologous semen diluted in egg-yolk diluent, the fertility of animals which had uterine titres was significantly lower (P\ m=ge\ 0\m=.\05) than that of animals which had no titres.Antibodies to homologous bovine semen were detected in very few uterine samples from animals which had received repeated inseminations.

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The Reproductive System

Shulman¹

1974

Molecular Biology Biochemistry and Biophysics

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Mechanism of Experimental Sterility Induced in Guinea Pigs by Injection of Homologous Testis and Sperm

Cited by 13 publications

References 3 publications

Anti‐sperm antibodies and reproductive failures

Anti‐sperm antibodies and reproductive failures

An Immune Response to Egg-Yolk Semen Diluent in Dairy Cows

The Reproductive System

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