Mechanism of cellular uptake and cytotoxicity of paclitaxel loaded lipid nanocapsules in breast cancer cells

Valsalakumari, Remya; Yadava, Sunil Kumar; Szwed, Marzena; Pandya, Abhilash D.; Mælandsmo, Gunhild Mari; Torgersen, Maria Lyngaas; Iversen, Tore-Geir; Skotland, Tore; Sandvig, Kirsten; Giri, Jyotsnendu

doi:10.1016/j.ijpharm.2021.120217

Cited by 27 publications

(15 citation statements)

References 59 publications

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“…K-RAS and H-RAS) (Belaid & Filippakis, 2021 ; Kay, 2021 ), small GTPases (e.g. Cdc42, Rac, Rab, and RhoA) (Garrett et al., 2000 ; Somsel Rodman & Wandinger-Ness, 2000 ; Pertz et al., 2006 ; Valsalakumari et al., 2021 ; Buckley et al., 2020 ), and phosphoinositides (Di Paolo & De Camilli, 2006 ; Walpole & Grinstein, 2020 ). Changes in the activity of these genes and related factors may play a role in the differences in macropinocytosis activity between various cell types.…”

Section: Discussionmentioning

confidence: 99%

Silmitasertib-induced macropinocytosis promoting DDP intracellular uptake to enhance cell apoptosis in oral squamous cell carcinoma

Song

Xia

et al. 2021

Drug Delivery

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Section: Discussionmentioning

confidence: 99%

Silmitasertib-induced macropinocytosis promoting DDP intracellular uptake to enhance cell apoptosis in oral squamous cell carcinoma

Song

Xia

et al. 2021

Drug Delivery

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“…A standard concentration of 10 ng/mL (concentration before base treatment) of PTX was used without and with treatment of different bases used in Methods I–IV. 10 ng/mL PTX was chosen to treat the cells for this study as this concentration is equivalent to the IC 50 of PTX in MCF‐7 cells 31 . Briefly, 5000 cells/well were seeded into 96‐well standard culture plates and allowed to attach overnight.…”

Section: Methodsmentioning

confidence: 99%

“…10 ng/mL PTX was chosen to treat the cells for this study as this concentration is equivalent to the IC 50 of PTX in MCF-7 cells. 31 Briefly, 5000 cells/well were seeded into 96-well standard culture plates and allowed to attach overnight. After 72 h of treatment, the viability of cells was determined by MTT assay as described in the previous section.…”

Section: Bioactivity Of Free Ptx Treated With Different Basesmentioning

confidence: 99%

A facile strategy for the preparation of polypropylene sulfide nanoparticles for hydrophobic and base‐sensitive cargo

Chauhan

Basu

Yadava

et al. 2021

J of Applied Polymer Sci

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Polypropylene sulfide (PPS) as reactive oxygen species (ROS) responsive nanoparticles (NPs) have attained great interest for drug delivery applications in many diseases such as cancer, pulmonary, neurovascular, cardiovascular, and age-related diseases. Despite great potential of PPS NPs as a nanocarrier, it remains relatively less explored for small molecules (drug) delivery, as the current method of PPS NPs synthesis involving strong bases like sodium methoxide (NaOMe) and 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU) results in breakdown of base sensitive drugs or molecules. Herein, we are reporting a facile synthesis method for PPS NPs preparation with high drug loading capacity (five times higher than the reported synthesis method) of base-sensitive cargos (Paclitaxel/Dil dye) within the PPS matrix and protection from possible breakdown of drug during synthesis. The NPs prepared via our method possess oxidative-responsive release of drug, preservation of drug bioactivity, and excellent storage stability at room temperature or 4 C. PPS NPs system demonstrate biocompatibility and drug-loaded NPs provide efficient anticancer activity against breast cancer cells. Our method of PPS NPs synthesis is suitable to prepare a delivery system with higher loading capacity of drug and small molecules for different biomedical applications.

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“…The 96-well-plates were incubated at 5% CO 2 with 37 ± 0.5 • C for 24, 48, and 72 h. After which, 20 µL (10 mg/mL) of MTT solution was added and incubated for 1 h to form formazone crystals. DMSO (100 µL) was added to dissolve the developed crystals after removing the media and subjected to Optical Density (OD) measurement using a microplate reader (Thermo Scientific ® Varioskan Flash Multimode Reader) at 570 nm [30].…”

Section: In Vitro Cellular Studies 2121 In Vitro Cytotoxicity Studiesmentioning

confidence: 99%

“…The cells were trypsinized, and the Qu was extracted using chloroform. The chloroform was evaporated, and the samples were reconstituted in a mobile phase and analyzed using the developed HPLC method, as described in Section 2.2 [30].…”

Section: In Vitro Cellular Studies 2121 In Vitro Cytotoxicity Studiesmentioning

confidence: 99%

Anti-Proliferative Potential of Quercetin Loaded Polymeric Mixed Micelles on Rat C6 and Human U87MG Glioma Cells

et al. 2022

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Quercetin (Qu) is a natural flavonoid present in many commonly consumed food items and is also identified as a potential anticancer agent. The present study evaluates the Qu-loaded polymeric mixed micelles (Qu-PMMs) against C6 and U87MG glioma cell lines. The Box–Behnken Design (BBD) was employed to study the influence of independent variables such as Soluplus, Vitamin-E polyethyleneglycol-1000 succinate (E-TPGS), and poloxamer 407 concentrations on dependent variables including particle size (PS), polydispersity index (PDI), and percentage entrapment efficiency (%EE) of the prepared Qu-PMMs. The Qu-PMMs were further characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Scanning Electron Microscope (SEM), and were assessed for in vitro drug release, effect on cell viability, migration, cellular uptake, and apoptosis assays. The PS, PDI, and % EE of the optimized PMMs were 107.16 ± 1.06 nm, 0.236 ± 0.053, and 77.46 ± 1.94%, respectively. The FTIR and XRD revealed that the Qu was completely entrapped inside the PMMs. The SEM analysis confirmed the spherical shape of micelles. The in vitro cell viability study showed that the Qu-PMMs had 1.7 times higher cytotoxicity against C6 and U87MG cells than Qu pure drug (Qu-PD). Furthermore, Qu-PMMs demonstrated superior cellular uptake, inhibited migration, and induced apoptosis when tested against C6 and U87MG cells than pure Qu. Thus, the polymeric mixed micelle (PMMs) enhanced the therapeutic effect of Qu and can be considered an effective therapeutic strategy to treat Glioma.

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Mechanism of cellular uptake and cytotoxicity of paclitaxel loaded lipid nanocapsules in breast cancer cells

Cited by 27 publications

References 59 publications

Silmitasertib-induced macropinocytosis promoting DDP intracellular uptake to enhance cell apoptosis in oral squamous cell carcinoma

Silmitasertib-induced macropinocytosis promoting DDP intracellular uptake to enhance cell apoptosis in oral squamous cell carcinoma

A facile strategy for the preparation of polypropylene sulfide nanoparticles for hydrophobic and base‐sensitive cargo

Anti-Proliferative Potential of Quercetin Loaded Polymeric Mixed Micelles on Rat C6 and Human U87MG Glioma Cells

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