Mechanism of action, sources, and application of peroxidases

Oliveira, Francine Kerstner de; Santos, Lucielen Oliveira; Garda‐Buffon, Jaqueline

doi:10.1016/j.foodres.2021.110266

Cited by 68 publications

(31 citation statements)

References 92 publications

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“…According to research and statistics, about 75 percent of the information that a person receives from the outside world is from vision. With the rapid development of science and technology, sports technology has played an important role in national leadership and sports [2]. With the introduction of the use of technology in sports training and the emergence of new research to make a difference in the study of physical education, training teaching training has not been completed previously.…”

Section: Introductionmentioning

confidence: 99%

Tennis Technology Recognition and Training Attitude Analysis Based on Artificial Intelligence Sensor

2022

Journal of Sensors

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In order to solve the problem of traditional tennis serving technique teaching, subjective, and experience-based teaching methods, the author proposes a method to extract tennis training movements based on artificial intelligence sensor video analysis. This method requires the use of ordinary cameras and computer technology; video analysis technology is used to guide tennis teaching and training. The result obtained is as follows: in the experimental group and the control group, at the end of the teaching stage, the movement characteristics of each link of the body are quite different, which can reach 15%-20%; when the experimental group and the control group did the “scratching back” movement during the teaching period, at the end of teaching, the students in the experimental group were close to high-level tennis players in their movement skills, and the movement gap was only about 5%. It is proved that the method proposed by the author provides an objective and scientific basis for measuring the referee’s penalty level. In the grass-roots tennis teaching and training, it has important value and significance and has a great promotion prospect.

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Section: Introductionmentioning

confidence: 99%

Tennis Technology Recognition and Training Attitude Analysis Based on Artificial Intelligence Sensor

2022

Journal of Sensors

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“…Peroxidases catalyze redox reactions on a wide range of substrates, and have potential industrial and biotechnological applications ( Regalado et al, 2004 ; Lopes et al, 2014 ; de Oliveira et al, 2021 ). HRP C1A (hereafter referred to as HRP), the major enzyme among the 19 isoenzymes produced in horseradish root ( Krainer and Glieder, 2015 ), is one of the most extensively studied peroxidases.…”

Section: Resultsmentioning

confidence: 99%

Production of Recombinant Horseradish Peroxidase in an Engineered Cell-free Protein Synthesis System

Park

Kim

2021

Front. Bioeng. Biotechnol.

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One of the main advantages of a cell-free synthesis system is that the synthetic machinery of cells can be modularized and re-assembled for desired purposes. In this study, we attempted to combine the translational activity of Escherichia coli extract with a heme synthesis pathway for the functional production of horseradish peroxidase (HRP). We first optimized the reaction conditions and the sequence of template DNA to enhance protein expression and folding. The reaction mixture was then supplemented with 5-aminolevulinic acid synthase to facilitate co-synthesis of the heme prosthetic group from glucose. Combining the different synthetic modules required for protein synthesis and cofactor generation led to successful production of functional HRP in a cell-free synthesis system.

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“…For example, Trichoderma fungi can synthesize several enzymes, such as peroxidase, chitinase, β-1, 3-glucanase, and phenylalanine ammonia-lyase, to overcome environmental stresses [40][41][42]. Previous works have shown the involvement of peroxidases in mycotoxin (especially OTA and AFB1) degradation and decontamination [43][44][45] as they are capable of oxidizing aflatoxins into polar and less toxic molecules [46]. The fungal strain and growing condition (i.e., media) affect the Trichoderma's ability to produce peroxidases [47].…”

Section: Peroxidases Productionmentioning

confidence: 99%

“…The quantification of OTA was obtained by the HPLC method [46]. The chromatographic separations were carried out in the isocratic mode using 65% phase A (water + 1% acetic acid) and 35% phase B (acetonitrile + 1% acetic acid) (Sigma-Aldrich).…”

Section: Ochratoxin a Quantificationmentioning

confidence: 99%

Trichoderma Enzymes for Degradation of Aflatoxin B1 and Ochratoxin A

et al. 2022

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The contamination of agricultural products with mycotoxins causes risks to animal and human health and severe economic losses. Mycotoxicoses can be reduced by preventing fungal infection using chemical and biological approaches. The chemical strategies can release toxic molecules; therefore, strategies for biological control are being evaluated, such as using nontoxic fungi and their metabolites. This work evaluated the effect of exoenzymes produced by the beneficial fungus Trichoderma afroharzianum strain T22 in degrading Aflatoxin B1 (AFB1) and Ochratoxin A (OTA). The ability of Trichoderma to produce hydrolases was stimulated by using different inducing substrates. The highest AFB1 and OTA degradation activity was obtained using a medium containing lyophilized mushrooms and crude fiber. The T. afroharzianum T22’s ability to reduce mycotoxins may be attributed to peroxidase enzymes. This study showed that T.afroharzianum strain T22 or its peroxidase supplementation could represent a sustainable strategy for the degradation of AFB1 and OTA in feed and food products.

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Mechanism of action, sources, and application of peroxidases

Cited by 68 publications

References 92 publications

Tennis Technology Recognition and Training Attitude Analysis Based on Artificial Intelligence Sensor

Tennis Technology Recognition and Training Attitude Analysis Based on Artificial Intelligence Sensor

Production of Recombinant Horseradish Peroxidase in an Engineered Cell-free Protein Synthesis System

Trichoderma Enzymes for Degradation of Aflatoxin B1 and Ochratoxin A

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