Mechanism of Action of Chlorophyllin against Mitomycin-C Mutagenicity in Allium cepa

Rigonato, Janaina; Mantovani, Mário; Jordão, Berenice Quinzani

doi:10.1508/cytologia.69.459

Cited by 5 publications

(4 citation statements)

References 24 publications

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“…These, among other agents, can lead to the appearance of cellular abnormalities (Rigonato et al, 2004). Therefore, we verified the need to develop preventive strategies by means of studies that identify substances with chemoprotective potential for the genetic material (De Flora and Ferguson, 2005).…”

Section: Introductionmentioning

confidence: 58%

“…This event is based on the principle that the growth of roots is inhibited when they are exposed to toxic substances (Fiskesjö, 1993a(Fiskesjö, ,b, 1994Rigonato et al, 2004). According to the reference values of Fiskesjö (1993a), the MIs that were calculated in the mutagenicity protocols show that the inulin does not have genotoxic activity in any of the three concentrations.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of the antimutagenic activity and mode of action of the fructooligosaccharide inulin in the meristematic cells of Allium cepa culture

Mauro¹,

Pesarini²,

Marin-Morales³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. This study evaluated the mutagenicity and antimutagenicity of inulin in a chromosomal aberration assay in cultures of the meristematic cells of Allium cepa. The treatments evaluated were as follows: negative control -seed germination in distilled water; positive control -aqueous solution of methyl methanesulfonate (10 μg/mL MMS); mutagenicity -aqueous solutions of inulin (0.015, 0.15, and 1.50 μg/mL); and antimutagenicity -associations between MMS and the different inulin concentrations. The antimutagenicity protocols established were pretreatment, simultaneous simple, simultaneous with pre-incubation, and post-treatment. The damage reduction percentage (DR%) was 43.56, 27.77, and 55.92% for the pre-treatment; -31.11, 18.51, and 7.03% for the simultaneous simple; 30.43, 19.12, and 21.11% for the simultaneous with pre-incubation; and 64.07, 42.96, and 53.70% for the post-treatment. The results indicated that the most effective treatment for inhibiting damages caused by MMS was the post-treatment, which was followed by the pretreatment, suggesting activity by bioantimutagenesis and desmutagenesis.The Allium cepa assay was demonstrated to be a good screening test for this type of activity because it is easy to perform, has a low cost, and shows DR% that is comparable to that reported studies that evaluated the prevention of DNA damage in mammals by inulin.

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Section: Introductionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Evaluation of the antimutagenic activity and mode of action of the fructooligosaccharide inulin in the meristematic cells of Allium cepa culture

Mauro¹,

Pesarini²,

Marin-Morales³

et al. 2014

Genet. Mol. Res.

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“…Interest in discovering natural agents that can protect DNA against damage induced by xenobiotics is increasing (Rigonato et al, 2004). Rosemary is an herb that contains agents with antimutagenic properties.…”

Section: Introductionmentioning

confidence: 99%

Mutagenic and antimutagenic effects of aqueous extract of rosemary (Rosmarinus officinalis L.) on meristematic cells of Allium cepa

Felicidade¹,

Lima²,

Pesarini³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Polyphenolic compounds present in rosemary were found to have antioxidant properties, anticarcinogenic activity, and to 9987©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (4): 9986-9996 (2014) Effects of rosemary on Allium cepa increase the detoxification of pro-carcinogens. The aim of the study was to determine the effect the aqueous extract of rosemary (AER) on mutagenicity induced by methylmethane sulfonate in meristematic cells of Allium cepa, as well as to describe its mode of action. Anti-mutagenicity experiments were carried out with 3 different concentrations of AER, which alone showed no mutagenic effects. In antimutagenicity experiments, AER showed chemopreventive activity in cultured meristematic cells of A. cepa against exposure to methylmethane sulfonate. Additionally, post-treatment and simultaneous treatment using pre-incubation protocols were the most effective. Evaluation of different protocols and the percent reduction in DNA indicated bioantimutagenic as well desmutagenic modes of action for AER. AER may be chemopreventive and antimutagenic.

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“…Furthermore, Cardoso et al (2014), Neves et al (2014), Barbério et al (2009), Sturbelle et al (2008, and Rigonato et al (2005) classify the A. cepa test system as very efficient for the initial evaluation of the cytotoxic, mutagenic and antimutagenic potential of natural products.…”

Section: Introductionmentioning

confidence: 99%

Antimitotic and antimutagenic action of the Hymenaea stigonocarpa bark on dividing cells

et al. 2016

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The objective of this study was to evaluate the action of Hymenaea stigonocarpa bark hydroalcoholic extract against a mutagenic compound using A. cepa meristematic root cells as a test system. The treatment groups were: Negative Control (NC) -distilled water; Positive Control (PC) -paracetamol at a concentration of 0.008 mg/mL, Jatoba Control (JC) -aqueous fraction jatobá-do-cerrado at 0.5 or 1.0 or 1.5 mg/mL, and Simultaneous Treatment (ST) -jatobá-do-cerrado aqueous fraction at a concentration of 0.5 or 1.0 or 1.5 mg/mL associated with paracetamol solution at a concentration of 0.008 mg/mL. All groups were analyzed at 24 and 48 h. Five onion bulbs (five replications) were used for each treatment group. The root tips were fixed in Carnoy and slides prepared by the crush technique. Cells were analyzed throughout the cell cycle, totaling 5,000 for each treatment group at each exposure time. Mitotic indices were subjected to statistical analysis using the chi-square test (p<0.05). From the results it was found that the ST group, at the three concentrations, significantly potentiated the antiproliferative effect of the test system cells when compared to PC, NC and TJ at the three concentrations. Furthermore, the three ST concentrations significantly reduced the number of cell aberrations when compared to the number of aberrant cells obtained for the PC, demonstrating antimutagenic action on the A. cepa test system cells.Keywords: jatobá-do-cerrado, cytotoxicity, protector effect. Ação antimitótica e antimutagênica do ritidoma de Hymenaea stigonocarpa Mart ex Hayne na divisão celularResumo O objetivo do presente trabalho foi avaliar a ação do extrato hidroalcólico do ritidoma de Hymenaea stigonocarpa frente a um composto mutagênico, utilizando como sistema teste as células meristemáticas de raízes de A. cepa. Os grupos tratamentos avaliados foram: Controle Negativo (CN) -água destilada; Controle Positivo (CP) -paracetamol na concentração de 0,008 mg/mL, Controle Jatobá (CJ) -fração aquosa de jatobá-do-cerrado na concentração de 0,5 ou 1,0 ou 1,5 mg/mL, e Tratamento Simultâneo (TS) -fração aquosa de jatobá-do-cerrado na concentração de 0,5 ou 1,0 ou 1,5 mg/mL associada a solução de paracetamol na concentração de 0,008 mg/mL. Todos os grupos foram analisados nos tempos de 24 e 48 h. Para cada grupo tratamento cinco bulbos de cebolas (cinco repetições) foram utilizados. As radículas foram fixadas em Carnoy e as lâminas preparadas pela técnica de esmagamento. Analisaram-se células em todo ciclo celular, totalizando 5.000 para cada grupo tratamento em cada tempo de exposição. Os índices mitóticos obtidos foram submetidos à análise estatística do 05). A partir dos resultados verificou-se que o grupo TS, nas três concentrações, potencializou o efeito antiproliferativo significativo as células do sistema teste quando comparado ao CP, CN e TJ nas três concentrações. Ainda, o TS nas três concentrações reduziu de forma significativa o número de aberrações celulares quando comparado com o número de células aberrantes ob...

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Mechanism of Action of Chlorophyllin against Mitomycin-C Mutagenicity in Allium cepa

Cited by 5 publications

References 24 publications

Evaluation of the antimutagenic activity and mode of action of the fructooligosaccharide inulin in the meristematic cells of Allium cepa culture

Evaluation of the antimutagenic activity and mode of action of the fructooligosaccharide inulin in the meristematic cells of Allium cepa culture

Mutagenic and antimutagenic effects of aqueous extract of rosemary (Rosmarinus officinalis L.) on meristematic cells of Allium cepa

Antimitotic and antimutagenic action of the Hymenaea stigonocarpa bark on dividing cells

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