Mechanism of Action and Structure of Acid Deoxyribonuclease

Bernardi, Giorgio

doi:10.1002/9780470122761.ch1

Cited by 11 publications

(2 citation statements)

References 109 publications

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“…The hybridized double stranded region of the DNA-MPs was expected to be cleaved by DNase II to release CpG ODNs efficiently from the mismatched region, due to the preference of DNase II to cleave double stranded regions than that of single strands under acidic conditions. 27 …”

Section: Resultsmentioning

confidence: 99%

“…Because DNase II is an abundant nuclease that exists in the phago-lysosomal compartment, it could mediate the release of CpG ODNs from the DNA-MPs by cleaving not single-stranded CpGs in the mismatched region, but double-stranded template DNA strands for successful interactions with TLR-9. 27,34 For this reason, we incubated DNA-MP-1.0 with DNase II for 4 h at acidic pH (pH 4.8) and physiological pH (pH 7.2) to mimic the conditions inside of the phagolysosome and extracellular region/cytoplasm. 35,36 After treatment of the DNA-MPs with several different concentrations of DNase II, the cleaved DNA-MPs were analyzed by gel electrophoresis.…”

Section: Resultsmentioning

confidence: 99%

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CpG incorporated DNA microparticles for elevated immune stimulation for antigen presenting cells

et al. 2018

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

CpG incorporated DNA microparticles for elevated immune stimulation for antigen presenting cells

et al. 2018

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Resolution of synthetic Holliday junctions in DNA by an endonuclease activity from calf thymus.

Elborough¹,

West²

1990

The EMBO Journal

103

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Extracts of calf thymus have been fractionated to reveal a nuclease activity that specifically cleaves model Holliday junctions in vitro. The products of cleavage are unbranched linear duplex DNA molecules. Using synthetic four‐way junctions, we show that the major sites of cutting are diametrically opposed, at sites one nucleotide from the base of the junction. Other types of four‐way junctions, including pseudo‐cruciform structures and cruciforms extruded from supercoiled plasmids, are also cleaved by the nuclease. The Mr of the partially purified activity, determined by gel filtration, is approximately 75,000. The calf thymus enzyme provides the first example of an endonuclease from a higher eukaryote that acts specifically on branch points in DNA, and indicates that junction‐resolving proteins are normal constituents of somatic cells.

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Endonuclease Activation and Chromosomal DNA Fragmentation during Apoptosis in Leukemia Cells

2006

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Apoptotic endonuclease is a key enzyme that mediates regulated DNA fragmentation and chromatin condensation in response to apoptotic signals such as the Fas ligand, ionizing radiation, and anticancer agents. An endonuclease that is activated specifically by caspase-3 has been identified in humans and mice. The human gene for this protein has been termed DFF40 (DNA fragmentation factor, 40-kd subunit) or caspase-activated nuclease (CPAN), whereas the mouse homologue has been named caspase-activated deoxyribonuclease (CAD). Although CAD/DFF40 is known as a major apoptotic nuclease, mice lacking inhibitor of CAD (ICAD) (also known as DFF45) are viable and still show DNA fragmentation, suggesting that alternative endonucleases play an important role during apoptosis. Endonuclease G has been reported to possibly be responsible for DNA fragmentation in various cells during apoptosis. Furthermore, we also have found that apurinic/apyrimidinic endonuclease 1 (Ape1) and its N-terminal-truncated form (AN34) are involved in DNA fragmentation during apoptosis in leukemia cells. In this review, we describe the features of several endonucleases that are involved in the apoptosis of human leukemia cells. Apoptotic endonuclease may vary among different leukemia cell types.

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Mechanism of Action and Structure of Acid Deoxyribonuclease

Cited by 11 publications

References 109 publications

CpG incorporated DNA microparticles for elevated immune stimulation for antigen presenting cells

CpG incorporated DNA microparticles for elevated immune stimulation for antigen presenting cells

Resolution of synthetic Holliday junctions in DNA by an endonuclease activity from calf thymus.

Endonuclease Activation and Chromosomal DNA Fragmentation during Apoptosis in Leukemia Cells

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