Resolution of synthetic Holliday junctions in DNA by an endonuclease activity from calf thymus.

Elborough, Kieran; West, Stephen C.

doi:10.1002/j.1460-2075.1990.tb07484.x

Cited by 103 publications

(70 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The evidence for diagonal digestion of cruciform DNA suggested that the resolution activity for Hollidayjunction structures in mammalian cells 28 could participate in this reaction. MUS81 nuclease, GEN1 and a complex of SLX4-SLX1 have been proposed as candidates for such a resolvase in mammalian cells [29][30][31][32][33][34][35][36][37] .…”

Section: Resultsmentioning

confidence: 99%

Two sequential cleavage reactions on cruciform DNA structures cause palindrome-mediated chromosomal translocations

et al. 2013

View full text Add to dashboard Cite

Gross chromosomal rearrangements (GCRs), such as translocations, deletions or inversions, are often generated by illegitimate repair between two DNA breakages at regions with nucleotide sequences that might potentially adopt a non-B DNA conformation. We previously established a plasmid-based model system that recapitulates palindrome-mediated recurrent chromosomal translocations in humans, and demonstrated that cruciform DNA conformation is required for the translocation-like rearrangements. Here we show that two sequential reactions that cleave the cruciform structures give rise to the translocation: GEN1-mediated resolution that cleaves diagonally at the four-way junction of the cruciform and Artemismediated opening of the subsequently formed hairpin ends. Indeed, translocation products in human sperm reveal the remnants of this two-step mechanism. These two intrinsic pathways that normally fulfil vital functions independently, Holliday-junction resolution in homologous recombination and coding joint formation in rearrangement of antigen-receptor genes, act upon the unusual DNA conformation in concert and lead to a subset of recurrent GCRs in humans.

show abstract

Section: Resultsmentioning

confidence: 99%

Two sequential cleavage reactions on cruciform DNA structures cause palindrome-mediated chromosomal translocations

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Although eukaryotic Holliday junction resolvases have not yet been identified, related activities have been observed during the fractionation of mammalian extracts (Elborough and West 1990;Hyde et al 1994), indicating that similar junction-processing events are likely to occur in eukaryotes. Our data indicate that factors that affect the assembly of the resolvase at the site of the junction may affect the outcome of a recombination event.…”

Section: Discussionmentioning

confidence: 99%

Assembly of the Escherichia coli RuvABC resolvasome directs the orientation of Holliday junction resolution

Gool¹,

Hajibagheri²,

Stasiak³

et al. 1999

Genes & Development

Self Cite

View full text Add to dashboard Cite

Genetic recombination can lead to the formation of intermediates in which DNA molecules are linked by Holliday junctions. Movement of a junction along DNA, by a process known as branch migration, leads to heteroduplex formation, whereas resolution of a junction completes the recombination process. Holliday junctions can be resolved in either of two ways, yielding products in which there has, or has not, been an exchange of flanking markers. The ratio of these products is thought to be determined by the frequency with which the two isomeric forms (conformers) of the Holliday junction are cleaved. Recent studies with enzymes that process Holliday junctions in Escherichia coli, the RuvABC proteins, however, indicate that protein binding causes the junction to adopt an open square-planar configuration. Within such a structure, DNA isomerization can have little role in determining the orientation of resolution. To determine the role that junction-specific protein assembly has in determining resolution bias, a defined in vitro system was developed in which we were able to direct the assembly of the RuvABC resolvasome. We found that the bias toward resolution in one orientation or the other was determined simply by the way in which the Ruv proteins were positioned on the junction. Additionally, we provide evidence that supports current models on RuvABC action in which Holliday junction resolution occurs as the resolvasome promotes branch migration.

show abstract

“…Direct Association of RAD51C and XRCC3 with HJ Resolvase Activity-The resolvase activity present in HeLa and hamster cell extracts has also been detected in a variety of calf and rabbit organ tissues (thymus, testis, and spleen) (37)(38)(39). An identical activity is found in plant extracts, such as those obtained from wheat germ, giving us a unique opportunity to determine whether exogenous expression of RAD51C (effectively using an in vitro transcription/translation system) in a wheat germ extract stimulates or blocks the endogenous resolvase activity present in the extract.…”

Section: Interaction Of the Resolvase Complex With Holliday Junctions-mentioning

confidence: 99%

Role of RAD51C and XRCC3 in Genetic Recombination and DNA Repair

Liu¹,

Tarsounas²,

O'Regan³

et al. 2007

Journal of Biological Chemistry

106

View full text Add to dashboard Cite

In germ line cells, recombination is required for gene reassortment and proper chromosome segregation at meiosis, whereas in somatic cells it provides an important mechanism for the repair of DNA double-strand breaks. Five proteins (RAD51B, RAD51C, RAD51D, XRCC2, and XRCC3) that share homology with RAD51 recombinase and are known as the RAD51 paralogs are important for recombinational repair, as paralog-defective cell lines exhibit spontaneous chromosomal aberrations, defective DNA repair, and reduced gene targeting. The paralogs form two distinct protein complexes, RAD51B-RAD51C-RAD51D-XRCC2 and RAD51C-XRCC3, but their precise cellular roles remain unknown. Here, we show that, like MLH1, RAD51C localized to mouse meiotic chromosomes at pachytene/diplotene. Using immunoprecipitation and gel filtration analyses, we found that Holliday junction resolvase activity associated tightly and co-eluted with the 80-kDa RAD51C-XRCC3 complex. Taken together, these data indicate that the RAD51C-XRCC3-associated Holliday junction resolvase complex associates with crossovers and may play an essential role in the resolution of recombination intermediates prior to chromosome segregation.

show abstract

Resolution of synthetic Holliday junctions in DNA by an endonuclease activity from calf thymus.

Cited by 103 publications

References 43 publications

Two sequential cleavage reactions on cruciform DNA structures cause palindrome-mediated chromosomal translocations

Two sequential cleavage reactions on cruciform DNA structures cause palindrome-mediated chromosomal translocations

Assembly of the Escherichia coli RuvABC resolvasome directs the orientation of Holliday junction resolution

Role of RAD51C and XRCC3 in Genetic Recombination and DNA Repair

Contact Info

Product

Resources

About