Mechanism for Inhibition of Folic Acid Photodecomposition by Various Antioxidants

Wusigale,; Hu, Lyulin; Cheng, Hao; Gao, Yahui; Liang, Li

doi:10.1021/acs.jafc.9b06263

Cited by 14 publications

(10 citation statements)

References 53 publications

(127 reference statements)

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“…Compounds known generically as antioxidants (flavonoids, cinnamic acids, stilbene, etc.) can influence the breakdown of folic acid, proving to be useful protectors [20]. Folates found in plasma have low affinity for proteins, instead folic acid could react with several proteins: through both hydrophobic and hydrophilic interactions it can interact with human and bovine serum albumin (due to tryptophan in their structure), with lactoferrin forming saturated reactive complexes, with β-casein forming stable complexes due to certain hydrophobic parts that are found in both structures [21][22][23].…”

Section: Chemical Perspectivesmentioning

confidence: 99%

Folates and Anti-folates � Chemical Perspectives, in vitro and in ovo Assessments

Sorop¹,

David²,

Heghes³

et al. 2020

Rev. Chim.

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Folates participate in DNA replication reactions, act as a substrate in enzymatic reactions related to amino acid synthesis and vitamin metabolism while antifolates participate in reactions that inhibit the formation of tetrahydrofolate with consequences on protein and nucleic acid synthesis and implicitly on growth and development both types of cells, healthy and diseased. In the present study, the viability of healthy cells, keratinocytes and human fibroblasts was evaluated in the presence of three folates (folic, dihydrofolic and tetrahydrofolic acids), one antifolate (methotrexate) and combinations between them by Alamar blue assay. The antiangiogenic potential was also evaluated by in ovo technique, CAM assay. Cell viability was influenced in a cell-dependent and dose-dependent manner, fibroblasts being more sensitive to the action of the test compounds, especially the combination of metrotrexate and dihydrofolate. Data related to CAM assay showed that methotrexate revealed a slightly higher vessel density, but without inducing toxicity on vascular architecture and functionality. The data obtained highlight the greater sensitivity of the viability of fibroblasts in the presence of metrotrexate and its combinations with folates used in the study.

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Section: Chemical Perspectivesmentioning

confidence: 99%

Folates and Anti-folates � Chemical Perspectives, in vitro and in ovo Assessments

Sorop¹,

David²,

Heghes³

et al. 2020

Rev. Chim.

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“…However, the stability of antioxidants could be improved through shielding them from the harsh environment upon complexation and encapsulation by proteins . Moreover, antioxidants may protect photosensitizers against photodecomposition . Therefore, the study on interaction among proteins, photosensitizers, and antioxidants would shed more light on the sensitization and stabilization mechanisms under light illumination, contributing to designing food products with desirable chemical stability.…”

Section: Introductionmentioning

confidence: 99%

“…β-Casein (24 kDa) accounts for about a third of total caseins, a major protein in bovine milk . The goal of this study is to explain the interaction between the proteins and FA under UVA irradiation in the absence and presence of CA, which at a concentration of 1 μM could reportedly inhibit the photodecomposition of 10 μM FA . The susceptibility of proteins to indirect photo-oxidation was characterized by amino acid composition and structural change.…”

Section: Introductionmentioning

confidence: 99%

Effects of Folic Acid and Caffeic Acid on Indirect Photo-oxidation of Proteins and Their Costabilization under Irradiation

Wusigale

Xiaojun

Yin

et al. 2021

J. Agric. Food Chem.

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Proteins, vitamins, and phenols are often present together in foods, but they are sensitive to environmental factors. Folic acid (FA), a synthetic form of folate, decomposes under light, leading to protein oxidation. Caffeic acid (CA), a phenolic acid, exhibits remarkable activity for scavenging reactive molecules. The exploitation of their interactions offers opportunities for designing the stabilizing system. In this study, FA-photodecomposition-induced protein (β-lactoglobulin, α-lactalbumin, bovine serum albumin, and β-casein) damage and its inhibition by CA were investigated in terms of protein composition and structural change. The results indicated that FA photoproducts oxidized the proteins via the electron transfer pathway, leading to degradation, aggregation, and unfolding. At the same time, photostability of FA, CA, and proteins in the tertiary mixture was better than that of any individual components. The antioxidant activity of the proteins contributed to their protection for FA. CA and its products inhibited FA photodecomposition and the photodecomposition-induced protein oxidation by trapping excited states.

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“…Although the topic of antioxidants (AO) research dates to the beginning of the last century, it is still relevant [ 1 , 2 , 3 ]. This is due to the great variety of biological mechanisms of antioxidant action in the human body [ 4 , 5 , 6 , 7 , 8 ] and, accordingly, the lack of possibility to create a universal method of antioxidant research. One of the relevant directions in this area is the creation of approaches to the determination of integral parameters of antioxidant and antiradical capacity/activity because the efficiency of the body’s antioxidant system may not be related to the content of a particular compound, but reflects the property of the system as a whole.…”

Section: Introductionmentioning

confidence: 99%

Study of Antioxidant Properties of Agents from the Perspective of Their Action Mechanisms

Ivanova

Газизуллина

2020

Molecules

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The creation and analysis of a large variety of existing methods for the evaluation of integrated antioxidant properties are quite relevant in connection with a range of biological mechanisms of the antioxidants (AO) action. In this work, the existing methods are correlated with mechanisms of antioxidant action. It is shown that the results obtained by various methods are mainly incomparable. This can be connected with the implementation of various mechanisms of antioxidant action in methods. The analysis of the literature data presented in this review indicates the difficulty of creating a universal method and the feasibility of using integrated approaches based on the use of several methods that implement and combine various mechanisms of the chemical conversion of antioxidants. This review describes methods for studying the chelating ability of antioxidants, except for methods based on electron and hydrogen atom transfer reactions, which are currently not widely covered in modern literature. With the description of each mechanism, special attention is paid to electrochemical methods, as the interaction of active oxygen metabolites of radical and non-radical nature with antioxidants has an electron/proton/donor-acceptor nature, which corresponds to the nature of electrochemical methods and suggests that they can be used to study the interaction.

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Mechanism for Inhibition of Folic Acid Photodecomposition by Various Antioxidants

Cited by 14 publications

References 53 publications

Folates and Anti-folates � Chemical Perspectives, in vitro and in ovo Assessments

Folates and Anti-folates � Chemical Perspectives, in vitro and in ovo Assessments

Effects of Folic Acid and Caffeic Acid on Indirect Photo-oxidation of Proteins and Their Costabilization under Irradiation

Study of Antioxidant Properties of Agents from the Perspective of Their Action Mechanisms

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