Partially mineralized fibrous tissue situated between tendon and bone is
believed to be tougher than either tendon or bone, possibly serving as a
compliant, energy absorptive, protective barrier between the two. This tissue
does not reform following surgical repair (e.g., rotator cuff tendon-to-bone
re-attachment) and might be a factor in the poor outcomes following such
surgeries. Towards our long-term goal of tissue engineered solutions to
functional tendon-to-bone re-attachment, we tested the hypotheses that partially
mineralized fibrous matrices can derive toughness from mobility of mineral along
their fibers, and that in such cases toughness is maximized at levels of
mineralization sufficiently low to allow substantial mobility. Nanofibrous
electrospun poly(lactic-co-glycolic acid) (PLGA) scaffolds mineralized for
prescribed times were fabricated as model systems to test these hypotheses.
Tensile tests performed at varying angles relative to the dominant fiber
direction confirmed that mineral cross-linked PLGA nanofibers without adhering
to them. Peel tests revealed that fracture toughness increased with
mineralization time up to a peak value, then subsequently decreased with
increasing mineralization time back to the baseline toughness of unmineralized
scaffolds. These experimental results were predicted by a theoretical model
combining mineral growth kinetics with fracture energetics, suggesting that
toughness increased with mineralization time until mineral mobility was
attenuated by steric hindrance, then returned to baseline levels following the
rigid percolation threshold. Results supported our hypotheses, and motivate
further study of the roles of mobile mineral particles in toughening the
tendon-to-bone attachment.