Mechanically robust photodegradable gelatin hydrogels for 3D cell culture and <i>in situ</i> mechanical modification

Norris, Sam C. P.; Delgado, Stephanie M.; Kasko, Andrea M.

doi:10.1039/c9py00308h

Cited by 26 publications

(37 citation statements)

References 76 publications

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“…GelMA is the methacrylation product of gelatin, which is a denatured form of collagen. [ 19b,26 ] We investigated the creation of GelMA‐DNA polymerization motor gels for potential tissue engineering applications.…”

Section: Resultsmentioning

confidence: 99%

“…GelMA hydrogels are widely used for cell adhesion, proliferation, and migration studies since they contain the RGD (arginine‐glycine‐aspartic acid) cell adhesive peptide motif. [ 19b ] Incorporating GelMA instead of Am or PEGDA as the copolymer enhances the biocompatibility of the gel for cell culture applications without adding peptides or proteins. [ 29 ] To validate cell compatibility of GelMA‐DNA gels, we cultured fluorescent HeLa GFP reporter cells and verified green fluorescence on GelMA‐DNA hydrogels.…”

Section: Resultsmentioning

confidence: 99%

“…Cell density will increase with increasing GelMA concentration, and despite the relatively low fractional concentration (5 wt%) of GelMA in our GelMA‐DNA hydrogel, we still observed high cell confluency after 2 days in culture. [ 19b ] Importantly, cells that were first adhered to as prepared GelMA‐DNA gels were viable even after DNA directed swelling was induced by adding a DNA hairpin solution to a final concentration of 10 × 10 −6 m (Figure 4d).…”

Section: Resultsmentioning

confidence: 99%

“…[ 1a,18 ] We developed gelatin‐based DNA polymerization motor hydrogels since gelatin is an irreversibly hydrolyzed form of collagen with good biocompatibility, cell adhesion, and biodegradation properties, and has previously been used to create cross‐linked matrices to study the behavior of cells in synthetic environments and is widely utilized in cell culture and tissue engineering. [ 19 ] These new multicomponent materials demonstrate how the DNA polymerization motor provides a general means to induce a stimuli‐responsive swelling behavior in hydrogels and open up new opportunities to use DNA sequences or other biomolecules to induce hydrogel shape change.…”

Section: Introductionmentioning

confidence: 99%

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Multicomponent DNA Polymerization Motor Gels

Shi

Fern

et al. 2020

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Hydrogels with the ability to change shape in response to biochemical stimuli are important for biosensing, smart medicine, drug delivery, and soft robotics. Here, a family of multicomponent DNA polymerization motor gels with different polymer backbones is created, including acrylamide‐co‐bis‐acrylamide (Am‐BIS), poly(ethylene glycol) diacrylate (PEGDA), and gelatin‐methacryloyl (GelMA) that swell extensively in response to specific DNA sequences. A common mechanism, a polymerization motor that induces swelling is driven by a cascade of DNA hairpin insertions into hydrogel crosslinks. These multicomponent hydrogels can be photopatterned into distinct shapes, have a broad range of mechanical properties, including tunable shear moduli between 297 and 3888 Pa and enhanced biocompatibility. Human cells adhere to the GelMA‐DNA gels and remain viable during ≈70% volumetric swelling of the gel scaffold induced by DNA sequences. The results demonstrate the generality of sequential DNA hairpin insertion as a mechanism for inducing shape change in multicomponent hydrogels, suggesting widespread applicability of polymerization motor gels in biomaterials science and engineering.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Multicomponent DNA Polymerization Motor Gels

Shi

Fern

et al. 2020

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“…[ 188 ] The o NB moiety has also been incorporated into N ‐hydroxysuccinimide‐terminated‐photocleavable PEG and methacrylated‐photocleavable‐gelatin. [ 189,190 ] PEGdiPDA has since been used to study the effect of organized and randomly arranged patterns of elastic moduli on hMSC and VIC differentiation and activation. [ 191,192 ]…”

Section: Mechanical Patterning Of Hydrogel Substratesmentioning

confidence: 99%

Surface Patterning of Hydrogel Biomaterials to Probe and Direct Cell–Matrix Interactions

Munoz‐Robles

Kopyeva

DeForest

2020

Adv Materials Inter

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Due to their mechanical and structural similarity to native tissues, hydrogel biomaterials have gained tremendous popularity for applications in 3D tissue culture, therapeutic screening, disease modeling, and regenerative medicine. Recent advances in pre‐ and post‐synthetic processing have afforded anisotropic manipulation of the biochemical, mechanical, and topographical properties of biocompatible gels, increasingly in a dynamic and heterogeneous fashion that mimics natural processes in vivo. Herein, the current state of hydrogel surface patterning to investigate cellular interactions with the surrounding matrix is reviewed, both in techniques utilized and biological findings explored, and the perspective on proposed future directions for the field is offered.

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Self‐Assembly and Mechanical Properties of Engineered Protein Based Multifunctional Nanofiber for Accelerated Wound Healing

George

Aarthy

Hemalatha

et al. 2021

Adv Healthcare Materials

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The present work reports a new route for preparing tunable multifunctional biomaterials through the combination of synthetic biology and material chemistry. Genetically encoded catechol moiety is evolved in a nanofiber mat with defined surface and secondary reactive functional chemistry, which promotes self-assembly and wet adhesion property of the protein. The catechol moiety is further exploited for the controlled release of boric acid that provides a congenial cellular microenvironment for accelerated wound healing. The presence of 3,4-dihydroxyphenylalanine in the nanofiber mat act as a stimulus to trigger cell proliferation, migration, and vascularization to accelerate wound healing. Electron paramagnetic resonance, NMR, FTIR, and circular dichroism spectroscopy confirm the structural integrity, antioxidant property, and controlled release of boric acid. Fluorescent and scanning electron microscopy reveals the 3D architecture of nanofiber mat, which favors fibroblast growth, endothelial cell attachment, and tube formation, which are the desirable properties of a wound-healing material. Animal studies in the murine wound healing model assert that the multifunctional biomaterial significantly improve re-epithelialization and accelerate wound closure.

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Mechanically robust photodegradable gelatin hydrogels for 3D cell culture and in situ mechanical modification

Abstract: Highly conjugated, hydrophobically modified gelatin hydrogels were synthesized, polymerized and degraded with orthogonal wavelengths of light.

Cited by 26 publications

References 76 publications

Multicomponent DNA Polymerization Motor Gels

Multicomponent DNA Polymerization Motor Gels

Surface Patterning of Hydrogel Biomaterials to Probe and Direct Cell–Matrix Interactions

Self‐Assembly and Mechanical Properties of Engineered Protein Based Multifunctional Nanofiber for Accelerated Wound Healing

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