Mechanical transmission of Tomato leaf curl New Delhi virus to cucurbit germplasm: selection of tolerance sources in Cucumis melo

López, Carmelo; Ferriol, M.; Picó, Maria Belén

doi:10.1007/s10681-015-1371-x

Cited by 72 publications

(112 citation statements)

References 27 publications

Supporting

Mentioning

109

Contrasting

Order By: Relevance

“…ToLCNDV-infected zucchini plants from Almeria were the original source of inoculum for mechanical inoculation as described in López et al (2015). The virus was transmitted to zucchini seedlings of the susceptible accession MU-CU-16 by virus-free whiteflies.…”

Section: Virus Sources For Mechanical Inoculationmentioning

confidence: 99%

“…With either inoculum from field or derived from the infectious clone, mechanical inoculation of ToLCNDV was performed as described in López et al (2015). Briefly, 1 g of infected zucchini leaf tissue was ground in inoculation buffer in a 1 : 4 (w : v) proportion.…”

Section: Mechanical Inoculationmentioning

confidence: 99%

“…Symptoms were assessed visually, using a scale from 0 (absence of symptoms) to 4 (very severe symptoms or dead plant) detailed in López et al (2015). The same conditions were used in the second assay, performed to confirm the response of some selected accessions, but symptoms were scored at 15 and 30 dpi.…”

Section: Symptoms Evaluation and Virus Detection By Polymerase Chain mentioning

confidence: 99%

“…Using this protocol, we demonstrated that this isolate has a wide host range, as it was successfully transmitted to 4 genera and 13 species of the Cucurbitaceae family, including the main crop species, such as cucumber, watermelon, melon, squash and zucchini, as well as crop-related exotic germplasm. The availability of this highly efficient method for mechanical transmission facilitated the identification of resistance in Indian melons (López et al, 2015). This resistance is now being used to develop resistant melon cultivars.…”

Section: Introductionmentioning

confidence: 99%

“…Losses in this crop are being especially devastating (Alfaro & Font, 2014;Janssen et al, 2014). The mechanical transmission method developed by López et al (2015) also allowed a preliminary study of the response of the main species of the genus Cucurbita. In general, the susceptibility of Cucurbita pepo L. was much higher than that of the other cultivated species of the genus, especially Cucurbita moschata Duchesne and Cucurbita maxima Duchesne.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Resistance to Tomato leaf curl New Delhi virus in Cucurbita spp.

Sáez

Martínez

Ferriol

et al. 2016

Annals of Applied Biology

View full text Add to dashboard Cite

Tomato leaf curl New Delhi virus (ToLCNDV) is a bipartite begomovirus (family Geminiviridae) first reported in India and its neighbouring countries. ToLCNDV severely affects zucchini crop (Cucurbita pepo) in the main production areas of Southern Spain since 2012. This emerging begomovirus is a serious threat to this and other cucurbit crops. Breeding resistant cultivars is the most promising method for disease control, but requires the identification of sources of resistance in the Cucurbita genus. In this work, we screened for ToLCNDV resistance a large collection of Cucurbita spp. accessions, including landraces and commercial cultivars of the main cultivated species, C. pepo, Cucurbita moschata and Cucurbita maxima and wild species. The screening was performed using mechanical and whitefly inoculation. The level of resistance was assessed by scoring symptom severity and by measuring the virus content with quantitative polymerase chain reaction in selected genotypes. Diversity in the response was observed within and among species. Severe symptoms and high viral amounts were found at 30 days after mechanical and whitefly inoculation in C. pepo, in all accessions belonging to the Zucchini morphotype and to other morphotypes of both subspecies, pepo and ovifera, and even in the wild relative Cucurbita fraterna. C. maxima was also highly susceptible. This species showed characteristic symptoms of leaf decay and intense yellowing, different from those of mosaic, curling and internode shortening found in C. pepo. The only species showing resistance was C. moschata. Four accessions were symptomless or had some plants with only mild symptoms after three independent rounds of mechanical inoculation with different inoculum sources. Two of them also remained symptomless after virus inoculation with viruliferous whiteflies. ToLCNDV was detected in these asymptomatic accessions at 15 and 30 days post inoculation, but viral amounts were much lower than those found in susceptible genotypes, suggesting a high level of resistance. The symptoms in the susceptible accessions of this species were also different, with a characteristic leaf mottling, evolving to a severe mosaic. The newly identified C. moschata resistant accessions are good candidates for breeding programmes to avoid the damage caused by ToLCNDV.

show abstract

Section: Virus Sources For Mechanical Inoculationmentioning

confidence: 99%

Section: Mechanical Inoculationmentioning

confidence: 99%

Section: Symptoms Evaluation and Virus Detection By Polymerase Chain mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Resistance to Tomato leaf curl New Delhi virus in Cucurbita spp.

Sáez

Martínez

Ferriol

et al. 2016

Annals of Applied Biology

View full text Add to dashboard Cite

show abstract

Breeding and genetics of resistance to major diseases in Cucurbita—A review

Sabharwal,

Thakur,

Shrestha

et al. 2024

Crop Science

View full text Add to dashboard Cite

Cucurbita crops, which include market types of pumpkin and squash, have unparalleled fruit variation and equally important economic value worldwide. Pumpkin and squash have versatile uses but are most popular in culinary, ornamental, snacking, and seed oil industries. The production of Cucurbita crops is hindered by diseases caused by fungal, oomycetes, and viral pathogens. Host resistance is an important component of integrated disease management for Cucurbita crops and is a major goal for plant breeders. This review addresses the major diseases of Cucurbita, including powdery mildew, downy mildew, Phytophthora rot, and aphid and whitefly transmitted viruses, with an emphasis on germplasm exploitation for the development of resistant cultivars. Resistance to powdery mildew derived from Cucurbita okeechobeensis subsp. martinezii (designated PM‐0) has been extensively deployed in commercial cultivars and was recently mapped on chromosomes 3 and 10 of Cucurbita moschata and Cucurbita pepo, respectively. Resistance to Phytophthora crown rot is present across several Cucurbita species, including Cucurbita lundelliana, C. okeechobeenesis subsp. okeechobeenesis, C. moschata, and C. pepo. Mapping studies have identified significant loci associated with Phytophthora crown rot resistance on chromosomes 4, 11, and 20 of C. moschata and chromosomes 4, 5, 8, 12, 13, 16, and 19 of C. pepo. Sources of resistance to aphid‐transmitted viruses exist in C. moschata, Cucurbita ficifolia, Cucurbita ecuadorensis, Cucurbita martinezii, C. ecuadorensis, Cucurbita maxima, and Cucurbita foetidissima. The availability of DNA markers linked to resistance against zucchini yellow mosaic virus and papaya ringspot virus in C. moschata and C. pepo has facilitated marker‐assisted selection (MAS) in breeding programs. On the other hand, sources of resistance to tomato leaf curl New Delhi virus (ToLCNDV), a major whitefly‐transmitted virus in Cucurbita, have been identified in C. moschata, C. lundelliana, and C. okeechobeensis. A major locus conferring resistance to ToLCNDV in C. moschata was recently mapped on chromosome 8 enabling the application of MAS with a prediction accuracy of 94.3%. Overall, the continued discovery and application of genomic tools for resistance breeding in Cucurbita will accelerate the rate of genetic gain while reducing costs associated with phenotyping.

show abstract