Mechanical Tissue Resuscitation Protects Against Myocardial Ischemia-Reperfusion Injury

Abstract

Treatment with mechanical tissue resuscitation during reperfusion reduces both early cell death and the delayed, programmed cell death after ischemia-reperfusion. This cardioprotection is also associated with a significant reduction in interstitial water. Additional cardioprotection may be derived from mechanical tissue resuscitation-induced increased blood flow. Mechanical tissue resuscitation, particularly with a resorbable device, is a straightforward and efficacious mechanical strategy for decreasing cardiomyocyte death following myocardial infarction as an adjunctive therapy to surgical revascularization.

Comparison of ketamine–dexmedetomidine–methadone and tiletamine–zolazepam–methadone combinations for short-term anaesthesia in domestic pigs

“…Short-term anaesthesia of domestic pigs is often required in biomedical research for minor surgical or diagnostic procedures (Hastings et al, 1982;Swindle et al, 1994;Nunes et al, 2007;Grasso et al, 2009;Staffieri et al, 2012;Jordan et al, 2014). The ideal anaesthetic protocol in pigs should provide fast and reliable immobilisation, minimal cardiovascular and respiratory depression, and adequate analgesia and muscle relaxation.…”

Mechanical Tissue Resuscitation (MTR): A Nonpharmacological Approach to Treatment of Acute Myocardial Infarction

“…MTR administered immediately upon reperfusion has previously been shown to reduce myocardial edema, infarct size, and apoptosis . Here, MTR reduced infarct size by nearly 50% even though treatment was delayed for 90 minutes (half of the total reperfusion period).…”

“…Hemodynamics and arterial blood gases were recorded at baseline, middle, and end of ischemia (mid‐ and end‐isc), as well as at 30 (R30), 60 (R60), 120 (R120), and 180 (R180) minutes of reperfusion. MTR‐treatment was applied 90 minutes after reperfusion and was accomplished using a polyvinyl–alcohol sponge, nitrile sheet, and a vacuum tube attached to a computer‐controlled vacuum pump as previously described . Evicel glue (Ethicon, Somerville, NJ, USA) was used to seal the edges of the nitrile to the myocardium creating an airtight patch.…”

“…Infarct size was determined by gravimetric analysis as previously described . Briefly, the coronary ligatures were permanently tightened and a blue dye was used to negatively stain the area at risk (AAR).…”

“…Regional myocardial blood flow was assessed at baseline, end of ischemia, R30, and R180 using neutron‐activated microspheres as previously described . Microspheres were injected centrally into the left atrium and reference blood samples were collected from the femoral artery.…”