Mechanical Strain Using 2D and 3D Bioreactors Induces Osteogenesis: Implications for Bone Tissue Engineering

Griensven, Martijn van; Diederichs, Solvig; Roeker, Stefanie; Boehm, Stefanie; Peterbauer, Anja; Wolbank, Susanne; Riechers, Daniel; Stahl, Frank; Kasper, Cornelia

doi:10.1007/10_2008_14

Cited by 10 publications

(9 citation statements)

References 71 publications

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“…It is proposed that in vivo mechanical loading can be used to increase bone formation in the scaffold instead of pre-seeding the scaffold with cells or delivering growth factors [33,34]. Mechanotransduction activates the mandibular osteoblast to promote remodeling and maintain the bone tissue [35].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Development of a biointegrated mandibular reconstruction device consisting of bone compatible titanium fiber mesh scaffold

et al. 2016

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Section: Accepted Manuscriptmentioning

confidence: 99%

Development of a biointegrated mandibular reconstruction device consisting of bone compatible titanium fiber mesh scaffold

et al. 2016

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“…Automated cell culture systems now exist and mechanical stimulation can be generated (van Griensven et al ., ; Tran et al ., ). Some relevant results obtained by means of bioreactors such as rotary cell culture (RCCS), rotating wall vessel (RWV) or random positioning machine (RPM) will be presented later in this review.…”

Section: Introductionmentioning

confidence: 99%

Bone mechanobiology, gravity and tissue engineering: effects and insights

Ruggiu

Cancedda

2014

J Tissue Eng Regen Med

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Bone homeostasis strongly depends on fine tuned mechanosensitive regulation signals from environmental forces into biochemical responses. Similar to the ageing process, during spaceflights an altered mechanotransduction occurs as a result of the effects of bone unloading, eventually leading to loss of functional tissue. Although spaceflights represent the best environment to investigate near-zero gravity effects, there are major limitations for setting up experimental analysis. A more feasible approach to analyse the effects of reduced mechanostimulation on the bone is represented by the 'simulated microgravity' experiments based on: (1) in vitro studies, involving cell cultures studies and the use of bioreactors with tissue engineering approaches; (2) in vivo studies, based on animal models; and (3) direct analysis on human beings, as in the case of the bed rest tests. At present, advanced tissue engineering methods allow investigators to recreate bone microenvironment in vitro for mechanobiology studies. This group and others have generated tissue 'organoids' to mimic in vitro the in vivo bone environment and to study the alteration cells can go through when subjected to unloading. Understanding the molecular mechanisms underlying the bone tissue response to mechanostimuli will help developing new strategies to prevent loss of tissue caused by altered mechanotransduction, as well as identifying new approaches for the treatment of diseases via drug testing. This review focuses on the effects of reduced gravity on bone mechanobiology by providing the up-to-date and state of the art on the available data by drawing a parallel with the suitable tissue engineering systems.

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“…Moreover, no physiological gradients of signaling molecules, metabolites, and oxygen can be created in 2D culture systems. For these reasons, 3D cultures are now increasingly used in basic research, drug screening, toxicity studies, and tissue engineering (TE) [1,2,3,4]. There are several 3D cell culture models that are used for these applications: scaffold-free cellular aggregates, cells growing on natural or synthetic scaffolds, and cells encapsulated in hydrogels [5,6].…”

Section: Introductionmentioning

confidence: 99%

Gelatin-Methacryloyl (GelMA) Formulated with Human Platelet Lysate Supports Mesenchymal Stem Cell Proliferation and Differentiation and Enhances the Hydrogel’s Mechanical Properties

et al. 2019

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Three-dimensional (3D) cell culture is a major focus of current research, since cultivation under physiological conditions provides more reliable information about in vivo cell behavior. 3D cell cultures are used in basic research to better understand intercellular and cell-matrix interactions. However, 3D cell culture plays an increasingly important role in the in vitro testing of bioactive substances and tissue engineering. Gelatin-methacryloyl (GelMA) hydrogels of different degrees of functionalization (DoFs) are a versatile tool for 3D cell culture and related applications such as bioprinting. Human platelet lysate (hPL) has already demonstrated positive effects on 2D cell cultures of different cell types and has proven a valuable alternative to fetal calf serum (FCS). Traditionally, all hydrogels are formulated using buffers. In this study, we supplemented GelMA hydrogels of different DoF with hPL during adipose tissue-derived mesenchymal stem cell (AD-MSCs) encapsulation. We studied the effect of hPL supplementation on the spreading, proliferation, and osteogenic differentiation of AD-MSCs. In addition, the influence of hPL on hydrogel properties was also investigated. We demonstrate that the addition of hPL enhanced AD-MSC spreading, proliferation, and osteogenic differentiation in a concentration-dependent manner. Moreover, the addition of hPL also increased GelMA viscosity and stiffness.

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Mechanical Strain Using 2D and 3D Bioreactors Induces Osteogenesis: Implications for Bone Tissue Engineering

Cited by 10 publications

References 71 publications

Development of a biointegrated mandibular reconstruction device consisting of bone compatible titanium fiber mesh scaffold

Development of a biointegrated mandibular reconstruction device consisting of bone compatible titanium fiber mesh scaffold

Bone mechanobiology, gravity and tissue engineering: effects and insights

Gelatin-Methacryloyl (GelMA) Formulated with Human Platelet Lysate Supports Mesenchymal Stem Cell Proliferation and Differentiation and Enhances the Hydrogel’s Mechanical Properties

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