Development of a biointegrated mandibular reconstruction device consisting of bone compatible titanium fiber mesh scaffold

Hirota, Makoto; Shima, Takaki; Sato, Itaru; Ozawa, Tomomichi; Iwai, Toshinori; Ametani, Akihiro; Sato, Mitsunobu; Noishiki, Yasuharu; Ogawa, Takahiro; Hayakawa, Tohru; Tohnai, Iwai

doi:10.1016/j.biomaterials.2015.09.034

Cited by 35 publications

(15 citation statements)

References 45 publications

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“…Thus, Ti microfiber scaffolds enable rapid and stable bone formation and integration while maintaining the shape of the bone reconstruction site. We previously reported that thin Ti microfiber scaffolds restored bone continuity of a segmental and critical-sized mandibular defects in rabbits [4]. However, it was reported that the bone formation ratio inside of the device was less than 50%, indicating that osteoconductivity into the device should be improved.…”

Section: Introductionmentioning

confidence: 97%

Tuning of Titanium Microfiber Scaffold with UV-Photofunctionalization for Enhanced Osteoblast Affinity and Function

Iwasaki

Hirota

Tanaka

et al. 2020

IJMS

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Titanium (Ti) is an osteoconductive material that is routinely used as a bulk implant to fix and restore bones and teeth. This study explored the effective use of Ti as a bone engineering scaffold. Challenges to overcome were: (1) difficult liquid/cell infiltration into Ti microfiber scaffolds due to the hydrophobic nature of Ti; and (2) difficult cell attachment on thin and curved Ti microfibers. A recent discovery of UV-photofunctionalization of Ti prompted us to examine its effect on Ti microfiber scaffolds. Scaffolds in disk form were made by weaving grade 4 pure Ti microfibers (125 µm diameter) and half of them were acid-etched to roughen the surface. Some of the scaffolds with original or acid-etched surfaces were further treated by UV light before cell culture. Ti microfiber scaffolds, regardless of the surface type, were hydrophobic and did not allow glycerol/water liquid to infiltrate, whereas, after UV treatment, the scaffolds became hydrophilic and immediately absorbed the liquid. Osteogenic cells from two different origins, derived from the femoral and mandibular bone marrow of rats, were cultured on the scaffolds. The number of cells attached to scaffolds during the early stage of culture within 24 h was 3–10 times greater when the scaffolds were treated with UV. The development of cytoplasmic projections and cytoskeletal, as well as the expression of focal adhesion protein, were exclusively observed on UV-treated scaffolds. Osteoblastic functional phenotypes, such as alkaline phosphatase activity and calcium mineralization, were 2–15 times greater on UV-treated scaffolds, with more pronounced enhancement on acid-etched scaffolds compared to that on the original scaffolds. These effects of UV treatment were associated with a significant reduction in atomic carbon on the Ti microfiber surfaces. In conclusion, UV treatment of Ti microfiber scaffolds tunes their physicochemical properties and effectively enhances the attachment and function of osteoblasts, proposing a new strategy for bone engineering.

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Section: Introductionmentioning

confidence: 97%

Tuning of Titanium Microfiber Scaffold with UV-Photofunctionalization for Enhanced Osteoblast Affinity and Function

Iwasaki

Hirota

Tanaka

et al. 2020

IJMS

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“…Consequently, the absence of bonding osteogenesis was attributed to the insufficient deposition of the apatite coating. We successfully coated a thin CA film inside the center area as well as the surface of TW using a molecular precursor solution [31][32][33] and found that CA-coated TW showed excellent bone formation [34][35][36][37] .…”

Section: Application Of the Molecular Precursor Methods Toward Three-dmentioning

confidence: 99%

“…They placed non-coated and CA-coated TWs under the periosteum of rat calvaria and confirmed greater bone formation and vertical bone formation rates of CA-coated TWs. Hirota et al 37) tried to use thin CA-coated TWs in the repair of segmental bone defects. They found that CA-coated TW is a bone compatible mandibular reconstruction device in immediately loaded segmental defects.…”

Section: Application Of the Molecular Precursor Methods Toward Three-dmentioning

confidence: 99%

Molecular precursor method for thin carbonate-containing apatite coating on dental implants

Hayakawa

Sato

2020

Dent. Mater. J.

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The molecular precursor method is an easy and simple method for coating thin carbonate-containing apatite (CA) films onto titanium surfaces. A molecular precursor solution containing ethanol, calcium-EDTA complex, and phosphate salt was dropped onto a titanium surface and then heated at 600°C for 2 h. An adherent thin CA coating was achieved. Animal implantation experiments showed that CA-coated implants had significantly higher bone-to-implant values than non-coated implants (p<0.05). The molecular precursor method was also used to coat three-dimensional titanium webs (TWs). Thin CA films could be coated inside the center area, as well as the surface of the TW, with excellent bone formation inside the CA-coated TW. Furthermore, the molecular precursor method was used to coat partially stabilized zirconia with CA. Better bone response was observed for CA-coated zirconia. From this, it is concluded that the molecular precursor method is useful for producing thin CA coatings on implant materials.

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“…Therefore, there are expectations that the recent advances in bone regenerative medicine will have practical use. As such, bone regenerative medicine requires a scaffold to serve as a basis for bone formation by retaining growth factors and cells; however, investigations of various materials have not yet find the most appropriate scaffold . Although conventional titanium plates are candidate scaffolds, bone forms on them and so they cannot be removed .…”

Section: Young's Moduli Of the Family Of Titanium Alloys And Human Comentioning

confidence: 99%

Titanium Fiber Plates for Bone Tissue Repair

et al. 2017

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Titanium plates are widely used in clinical settings because of their high bone affinity. However, owing to their high elastic modulus, these plates are not suitable for bone repair since their proximity to the bone surface for prolonged periods can cause stress shielding, leading to bone embrittlement. In contrast, titanium fiber plates prepared by molding titanium fibers into plates by simultaneously applying compression and shear stress at normal room temperature can have an elastic modulus similar to that of bone cortex, and stress shielding will not occur even when the plate lies flush against the bone's surface. Titanium fibers can form a porous structure suitable for cell adhesion and as a bone repair scaffold. A titanium fiber plate is combined with osteoblasts and shown that the titanium fiber plate is better able to facilitate bone tissue repair than the conventional titanium plate when implanted in rat bone defects. Capable of being used in close contact with bone for a long time, and even capable of promoting bone repair, titanium fiber plates have a wide range of applications, and are expected to make great contributions to clinical management of increasing bone diseases, including bone fracture repair and bone regenerative medicine.

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Development of a biointegrated mandibular reconstruction device consisting of bone compatible titanium fiber mesh scaffold

Cited by 35 publications

References 45 publications

Tuning of Titanium Microfiber Scaffold with UV-Photofunctionalization for Enhanced Osteoblast Affinity and Function

Tuning of Titanium Microfiber Scaffold with UV-Photofunctionalization for Enhanced Osteoblast Affinity and Function

Molecular precursor method for thin carbonate-containing apatite coating on dental implants

Titanium Fiber Plates for Bone Tissue Repair

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