Mechanical Stimulation Increases Knee Meniscus Gene RNA-level Expression in Adipose-derived Stromal Cells

Meier, Elizabeth M.; Wu, Bin; Siddiqui, Aamir; Tepper, Donna; Longaker, Michael T.; Lam, Mai T.

doi:10.1097/gox.0000000000000854

Cited by 13 publications

(10 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Despite this difference in energy release, we demonstrate here that ultrasound deployed via the two most commonly used UAL devices (VASER and Lysonix) allow the harvest of fully functional ASCs for regenerative medicine. In fact, in line with recent mechanobiology discoveries, mechanical stimulation could be a beneficial impulse for mesenchymal stem cells potentially enhancing their functionality [20] and our data suggests that ultrasonic energy deployed at 36kHZ may be beneficial for osteogenic differentiation of ACSs.…”

Section: Discussionsupporting

confidence: 85%

Ultrasound-assisted liposuction provides a source for functional adipose-derived stromal cells

et al. 2017

Self Cite

View full text Add to dashboard Cite

Regenerative medicine employs human mesenchymal stromal cells (MSCs) for their multi-lineage plasticity and their pro-regenerative cytokine secretome. Adipose derived mesenchymal stromal cells (ASCs) are concentrated in fat tissue and the ease of harvest via liposuction makes them a particularly interesting cell source. However, there are various liposuction methods and only few have been assessed regarding their impact on ASC functionality. Here we study the impact of the two most popular ultrasound-assisted liposuction (UAL) devices currently in clinical use VASER (Solta Medical; Hayward, CA, USA) and Lysonix 3000 (Mentor, Santa Barbara, CA, USA) on ASCs yield, viability, osteogenic and adipogenic differentiation capacity and in vivo regenerative performance. After lipoaspirate harvest and processing we sorted for ASCs using Fluorescent Assisted Cell Sorting (FACS) based on an established surface marker profile (CD34+CD31−CD45−). Both UAL samples demonstrated equivalent ASC yield and viability. VASER UAL ACSs showed higher osteogenic and adipogenic marker expression, however still a comparable differentiation capacity was observed. Soft tissue healing and neovascularization were significantly enhanced via both UAL derived ASCs in vivo and there was no significant difference between the cell therapy groups. Taken together, our data suggests that UAL allows safe and efficient harvesting of the mesenchymal stromal cellular fraction of adipose tissue and that cells harvested via this approach are suitable for cell therapy and tissue engineering applications.

show abstract

Section: Discussionsupporting

confidence: 85%

Ultrasound-assisted liposuction provides a source for functional adipose-derived stromal cells

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…51 Hence, it was also essential to investigate expression level of type I collagen as a specific marker of fibrogenic tissues which plays a key role in a tissue's ability to withstand tensile forces. 52 Here, a considerable level of COLL1A1 expression was observed which could indicate that seeded cells on PCL 0.2 /Ch/ECM group can differentiate toward a fibrochondrocyte phenotype and be used to improve meniscus injury outcomes. It was well documented that, fibrochondrocytes in inner part of meniscus, have a clear cell associated matrix (CAM) and synthesize large amount of fibrous type I collagen and lower of type II collagen.…”

Section: Discussionmentioning

confidence: 72%

Development of meniscus‐inspired 3D‐printed PCL scaffolds engineered with chitosan/extracellular matrix hydrogel

Asgarpour

Masaeli

Kermani

2021

Polymers for Advanced Techs

View full text Add to dashboard Cite

show abstract

“…62 In addition, mechanical stimulation, such as tensile strain, can promote the osteogenic, chondrogenic, tenogenic, and fibrogenic differentiation of stem cells in vitro. 7,17,42 Thus, we suspect that the ECM of an implanted USC sheet and the local tensile environment at the RC healing site may work together to induce the implanted cells partly to follow osteogenic, chondrogenic, tenogenic, angiogenic, and fibrogenic lineages, thereby allowing the IT enthesis in the USC sheet group to show better enhancements in healing quality. To prove these interpretations, further research will be required.…”

Section: Discussionmentioning

confidence: 99%

Application of Autogenous Urine-Derived Stem Cell Sheet Enhances Rotator Cuff Healing in a Canine Model

et al. 2020

Am J Sports Med

View full text Add to dashboard Cite

Background: A repaired rotator cuff (RC) often heals with interposed scar tissue, making repairs prone to failure. Urine-derived stem cells (USCs), with robust proliferation ability and multilineage differentiation, can be isolated from urine, avoiding invasive and painful surgical procedures for harvesting the cells. These advantages make it a novel cell source for autologous transplantation to enhance RC healing. Hypothesis: Implantation of an autogenous USC sheet to the injury site will enhance RC healing. Study Design: Controlled laboratory study. Methods: USCs isolated from urine were cultured using ascorbic acid and transforming growth factor β3 to form a cell sheet. Sixteen male mature beagles underwent bilateral shoulder surgery. The right shoulder underwent infraspinatus tendon (IT) insertion detachment and repair only, and the other was subjected to IT insertion detachment and repair, followed by autogenous USC sheet implantation. Among the animals, 3 received a Dil (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate)- labeled USC sheet implant in the right shoulder and were sacrificed at postoperative 6 weeks for cell tracking. The other animals were sacrificed at postoperative 12 weeks, and the IT-humerus complexes were harvested for gross observation, micro–computed tomography evaluation and histological analysis (n = 5), and mechanical testing (n = 8). Additionally, 13 unpaired canine cadaveric shoulders were included as native controls. Results: Micro–computed tomography analysis showed that the USC sheet group had a significant increase in bone volume/total volume and trabecular thickness at the RC healing site when compared with the control group ( P < .05 for all). Histologically, the Dil-labeled USC sheet was still visible at the RC healing site, which suggested that the implanted USCs remained viable at postoperative 6 weeks. Meanwhile, the healing interface in the USC sheet group regenerated significantly more enthesis-like tissue than did that of the control group ( P < .05). Additionally, the healing interface in the USC sheet group presented a larger fibrocartilage area, more proteoglycan deposition, and higher collagen birefringence than did that of the control group ( P < .05 for all). Biomechanically, the USC sheet group showed significantly higher failure load and stiffness versus the control group ( P < .05 for all). Conclusion: A USC sheet was able to enhance RC healing in a canine model. Clinical Relevance: The findings of the study showed that USC sheet implantation could serve as a practical application for RC healing.

show abstract

Mechanical Stimulation Increases Knee Meniscus Gene RNA-level Expression in Adipose-derived Stromal Cells

Abstract: Supplemental Digital Content is available in the text.

Cited by 13 publications

References 25 publications

Ultrasound-assisted liposuction provides a source for functional adipose-derived stromal cells

Ultrasound-assisted liposuction provides a source for functional adipose-derived stromal cells

Development of meniscus‐inspired 3D‐printed PCL scaffolds engineered with chitosan/extracellular matrix hydrogel

Application of Autogenous Urine-Derived Stem Cell Sheet Enhances Rotator Cuff Healing in a Canine Model

Contact Info

Product

Resources

About