Mechanical Stability Determines Stress Fiber and Focal Adhesion Orientation

Stamenović, Dimitrije; Lazopoulos, K.A.; Pirentis, Athanassios P.; Suki, Bélâ

doi:10.1007/s12195-009-0093-3

Cited by 35 publications

(42 citation statements)

References 34 publications

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“…The Poisson constant of basic mechanics describes how stretched objects get thinner, i.e., are compressed. Theoretical modeling of cytoskeleton reorganization under stretching has shown that the cytoskeleton resists loading mainly by changing the orientation and spacing of actin filaments (33). Longitudinal stretching along fibers causes reduction in the spacing between them (31).…”

Section: Discussionmentioning

confidence: 99%

Real-time observation of flow-induced cytoskeletal stress in living cells

Rahimzadeh

Meng

Sachs

et al. 2011

American Journal of Physiology-Cell Physiology

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The mechanical stress due to shear flow has profound effects on cell proliferation, transport, gene expression, and apoptosis. The mechanisms for flow sensing and transduction are unclear, but it is postulated that fluid flow pulls upon the apical surface, and the resulting stress is eventually transmitted through the cytoskeleton to adhesion plaques on the basal surface. Here we report a direct observation of this flow-induced stress in the cytoskeleton in living cells using a parallel plate microfluidic chip with a fluorescence resonance energy transfer (FRET)-based mechanical stress sensor in actinin. The sensing cassette was genetically inserted into the cytoskeletal host protein and transfected into Madin-Darby canine kidney cells. A shear stress of 10 dyn/cm(2) resulted in a rapid increase in the FRET ratio indicating a decrease in stress across actinin with flow. The effect was reversible, and cells were able to respond to repeated stimulation and showed adaptive changes in the cytoskeleton. Flow-induced Ca(2+) elevation did not affect the response, suggesting that flow-induced changes in actinin stress are insensitive to intracellular Ca(2+) level. The reduction in FRET ratio suggests actin filaments are under normal compression in the presence of flow shear stress due to changes in cell shape, and/or actinin is not in series with actin. Treatment with cytochalasin-D that disrupts F-actin reduced prestress and the response to flow. The FRET/flow method is capable of resolving changes of stress in multiple proteins with optical spatial resolution and time resolution >1 Hz. This promises to provide insight into the force distribution and transduction in all cells.

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Section: Discussionmentioning

confidence: 99%

Real-time observation of flow-induced cytoskeletal stress in living cells

Rahimzadeh

Meng

Sachs

et al. 2011

American Journal of Physiology-Cell Physiology

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“…7,11,62,64,67,69 While each model has provided insight into the process of stretchinduced stress fiber reorganization, only a few of these models are capable of addressing the effects of strain rate. Wei et al 69 proposed a chemo-mechanical model in which cells initially devoid of stress fibers quickly formed stress fibers oriented in the direction of least fiber shortening, i.e.…”

Section: Strain Rate-dependent Stress Fiber Reorientationmentioning

confidence: 99%

Multiple Roles for Myosin II in Tensional Homeostasis Under Mechanical Loading

Kaunas

Deguchi

2011

Cel. Mol. Bioeng.

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Cyclic stretching of adherent cells regulates cell morphology, signal transduction and cell function. It is well established that actin stress fibers are mechano-sensitive structural elements that reorganize in response to applied stress and strain. In this review, we discuss studies revealing the roles of myosin II in stress fiber remodeling including stress fiber assembly, disassembly, and tension maintenance. The results of these studies are interpreted with mathematical models that describe a mechanism by which stress fibers reorganize in response to cyclic stretch and predict how changes in stress fiber tension regulate signal transduction dependent on the spatial and temporal patterns of the applied strain.

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“…To address a biomechanical issue, mathematical models help elucidate the complex relations between SFO and different patterns of stretch (De et al, 2007, Deshpande et al, 2006Hsu et al, 2009;Stamenović et al, 2009). Based on different criteria, such as maintaining local strain in the surrounding matrix or SF, or attaining a global minimum energy, different models predicted that SF contractility, Poisson's ration of the substrate, or the strain frequency are the predominant parameters controlling SFO response to the exogenous uniaxial stretch, respectively.…”

Section: Introductionmentioning

confidence: 99%