Effects of stress fiber contractility on uniaxial stretch guiding mitosis orientation and stress fiber alignment

Zhao, Lei; Sang, Chen; Yang, Chun; Zhuang, Fengyuan

doi:10.1016/j.jbiomech.2011.06.033

Cited by 19 publications

(22 citation statements)

References 35 publications

(42 reference statements)

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“…Thus, the migration of intact keratocytes parallel to the stretching direction may be induced by the re-distribution of stress fibers, as seen in slow-crawling cells. [26][27][28][29][30][31][32] In this study, we have shown only indirect and statistical evidence (Figs. 2-4), and could not completely rule out other possibilities.…”

Section: Discussioncontrasting

confidence: 64%

“…[26][27][28][29][30][31][32] The cause of this reaction is thought to be the depolymerization of stress fibers aligned parallel to the direction of stretching by the binding of the actin-severing protein cofilin. 50,51 To test whether the depolymerization of stress fibers in response to the periodic stretching stimuli is required for the directional migration of keratocytes, we treated them with jasplakinolide, which inhibits the depolymerization of stress fibers composed of actin filaments.…”

Section: Jasplakinolide-treated Keratocytes Migrate In Random Directimentioning

confidence: 99%

“…In response to this periodic stretching of the elastic substratum, in slow-crawling cells such as fibroblasts, as well as endothelial, osteosarcoma, and smooth muscle cells, the intracellular stress fibers are rearranged perpendicular to the stretching direction, and the shape of the cells extends in that direction. [26][27][28][29][30][31][32] In neutrophil-like differentiated HL-60 cells and Dictyostelium cells, which have no stress fibers, and which show fast-crawling migration, periodic stretching of the elastic substratum makes them migrate perpendicular to the direction of stretching. 33,34 The chief cause of this directional migration was revealed by trajectory analysis, which is a powerful tool for identifying the behavioral strategy that crawling cells adopt for survival.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Hybrid mechanosensing system to generate the polarity needed for migration in fish keratocytes

Okimura

Iwadate

2016

Cell Adhesion & Migration

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Crawling cells can generate polarity for migration in response to forces applied from the substratum. Such reaction varies according to cell type: there are both fast-and slow-crawling cells. In response to periodic stretching of the elastic substratum, the intracellular stress fibers in slow-crawling cells, such as fibroblasts, rearrange themselves perpendicular to the direction of stretching, with the result that the shape of the cells extends in that direction; whereas fast-crawling cells, such as neutrophil-like differentiated HL-60 cells and Dictyostelium cells, which have no stress fibers, migrate perpendicular to the stretching direction. Fish epidermal keratocytes are another type of fastcrawling cell. However, they have stress fibers in the cell body, which gives them a typical slowcrawling cell structure. In response to periodic stretching of the elastic substratum, intact keratocytes rearrange their stress fibers perpendicular to the direction of stretching in the same way as fibroblasts and migrate parallel to the stretching direction, while blebbistatin-treated stress fiberless keratocytes migrate perpendicular to the stretching direction, in the same way as seen in HL-60 cells and Dictyostelium cells. Our results indicate that keratocytes have a hybrid mechanosensing system that comprises elements of both fast-and slow-crawling cells, to generate the polarity needed for migration.

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Section: Discussioncontrasting

confidence: 64%

Section: Jasplakinolide-treated Keratocytes Migrate In Random Directimentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Hybrid mechanosensing system to generate the polarity needed for migration in fish keratocytes

Okimura

Iwadate

2016

Cell Adhesion & Migration

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“…[3][4][5][6] In response to periodic stretching of the elastic substratum, intracellular stress fibers in fibroblasts, and endothelial, osteosarcoma, and smooth muscle cells rearrange themselves perpendicular to the direction of stretching, with the result that the shape of the cells extends in that direction. [7][8][9][10][11][12][13] It is also an interesting question as to whether or not the direction of cell crawling is regulated by periodic stretching of the substratum. We found recently that Dictyostelium cells, which are fast-crawling cells that have no stress fibers, migrate perpendicular to the direction of periodic stretching.…”

Section: Introductionmentioning

confidence: 99%

Fast-crawling cell types migrate to avoid the direction of periodic substratum stretching

Okimura

Ueda

Sakumura

et al. 2016

Cell Adhesion & Migration

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To investigate the relationship between mechanical stimuli from substrata and related cell functions, one of the most useful techniques is the application of mechanical stimuli via periodic stretching of elastic substrata. In response to this stimulus, Dictyostelium discoideum cells migrate in a direction perpendicular to the stretching direction. The origins of directional migration, higher migration velocity in the direction perpendicular to the stretching direction or the higher probability of a switch of migration direction to perpendicular to the stretching direction, however, remain unknown. In this study, we applied periodic stretching stimuli to neutrophil-like differentiated HL-60 cells, which migrate perpendicular to the direction of stretch. Detailed analysis of the trajectories of HL-60 cells and Dictyostelium cells obtained in a previous study revealed that the higher probability of a switch of migration direction to that perpendicular to the direction of stretching was the main cause of such directional migration. This directional migration appears to be a strategy adopted by fast-crawling cells in which they do not migrate faster in the direction they want to go, but migrate to avoid a direction they do not want to go.

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“…Several stress inducing protocols are well established to approach cytoskeleton behaviors, including fluid shear stress [13, 14], (simulated) micro-gravity [15, 16], cyclic stretch [11, 17, 18], among others. Cellular dynamics under such stress conditions are then studied by classical fluorescence or confocal microscopy, on fixed or alive cells.…”

Section: Introductionmentioning

confidence: 99%

A Robust Actin Filaments Image Analysis Framework

Alioscha-Pérez

Benadiba²,

Goossens

et al. 2016

PLoS Comput Biol

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The cytoskeleton is a highly dynamical protein network that plays a central role in numerous cellular physiological processes, and is traditionally divided into three components according to its chemical composition, i.e. actin, tubulin and intermediate filament cytoskeletons. Understanding the cytoskeleton dynamics is of prime importance to unveil mechanisms involved in cell adaptation to any stress type. Fluorescence imaging of cytoskeleton structures allows analyzing the impact of mechanical stimulation in the cytoskeleton, but it also imposes additional challenges in the image processing stage, such as the presence of imaging-related artifacts and heavy blurring introduced by (high-throughput) automated scans. However, although there exists a considerable number of image-based analytical tools to address the image processing and analysis, most of them are unfit to cope with the aforementioned challenges. Filamentous structures in images can be considered as a piecewise composition of quasi-straight segments (at least in some finer or coarser scale). Based on this observation, we propose a three-steps actin filaments extraction methodology: (i) first the input image is decomposed into a ‘cartoon’ part corresponding to the filament structures in the image, and a noise/texture part, (ii) on the ‘cartoon’ image, we apply a multi-scale line detector coupled with a (iii) quasi-straight filaments merging algorithm for fiber extraction. The proposed robust actin filaments image analysis framework allows extracting individual filaments in the presence of noise, artifacts and heavy blurring. Moreover, it provides numerous parameters such as filaments orientation, position and length, useful for further analysis. Cell image decomposition is relatively under-exploited in biological images processing, and our study shows the benefits it provides when addressing such tasks. Experimental validation was conducted using publicly available datasets, and in osteoblasts grown in two different conditions: static (control) and fluid shear stress. The proposed methodology exhibited higher sensitivity values and similar accuracy compared to state-of-the-art methods.

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Effects of stress fiber contractility on uniaxial stretch guiding mitosis orientation and stress fiber alignment

Cited by 19 publications

References 35 publications

Hybrid mechanosensing system to generate the polarity needed for migration in fish keratocytes

Hybrid mechanosensing system to generate the polarity needed for migration in fish keratocytes

Fast-crawling cell types migrate to avoid the direction of periodic substratum stretching

A Robust Actin Filaments Image Analysis Framework

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