Mechanical relaxations of hydrogels governed by their physical or chemical crosslinks

Cuenot, Stéphane; Gélébart, Perrine; Sinquin, Corinne; Colliec-Jouault, Sylvia; Zykwinska, Agata

doi:10.1016/j.jmbbm.2022.105343

Cited by 17 publications

(10 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…demonstrated macrophage polarization may be dependent on both stiffness and the crosslinking chemistry used to formulate the hydrogels. [67] They showed large differences in macrophage polarization based on whether the gel was physically or chemically crosslinked; however, Cuenot et al showed that crosslinking chemistry influences viscoelasticity, which was not explored by Sridharan et al [76] When hydrogel viscoelasticity was changed, T cell phenotype was impacted. [75]…”

Section: Mechanical Properties Of the Biomaterials Impact Immune Cell...mentioning

confidence: 99%

See 1 more Smart Citation

Engineering Biomaterials to Model Immune‐Tumor Interactions In Vitro

Skirzynska,

Xue,

Shoichet

2024

Advanced Materials

View full text Add to dashboard Cite

Engineered biomaterial scaffolds are becoming more prominent in research laboratories to study drug efficacy for oncological applications in vitro, but do they have a place in pharmaceutical drug screening pipelines? The low efficacy of cancer drugs in phase II/III clinical trials suggests that there are critical mechanisms not properly accounted for in the pre‐clinical evaluation of drug candidates. Immune cells associated with the tumour may account for some of these failures given recent successes with cancer immunotherapies; however, there are few representative platforms to study immune cells in the context of cancer as traditional 2D culture is typically monocultures and humanized animal models have a weakened immune composition. Biomaterials that replicate tumour microenvironmental cues may provide a more relevant model with greater in vitro complexity. In this review, we explore the pertinent microenvironmental cues which drive tumour progression in the context of the immune system, discuss how these cues can be incorporated into hydrogel design to culture immune cells, and describe progress toward precision oncological drug screening with engineered tissues.This article is protected by copyright. All rights reserved

show abstract

Section: Mechanical Properties Of the Biomaterials Impact Immune Cell...mentioning

confidence: 99%

“…showed that crosslinking chemistry influences viscoelasticity, which was not explored by Sridharan et al. [ 76 ] When hydrogel viscoelasticity was changed, T cell phenotype was impacted. [ 75 ]…”

Section: Biomaterials Scaffolds Modulate Immune Cell Phenotypementioning

confidence: 99%

Engineering Biomaterials to Model Immune‐Tumor Interactions In Vitro

Skirzynska,

Xue,

Shoichet

2024

Advanced Materials

View full text Add to dashboard Cite

show abstract

“…Then, force-distance curves were recorded on cells at a low constant speed of 1 µm/s to neglect the hydrodynamic drag forces exerted on the cantilever by the liquid medium. 49 These curves were then converted into force-indentation curves, and the approach part was fitted by the Hertz model to determine the apparent elastic modulus of cells. 50…”

Section: Atomic Force Microscopy (Afm) Experimentsmentioning

confidence: 99%

Identification of rare missense variants reducing cathepsin O secretion in families with intracranial aneurysm

Freneau

Blanchet

Benichi

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Intracranial aneurysm (IA) is a common cerebrovascular abnormality characterized by localized dilation and wall thinning in intracranial arteries, that frequently leads to fatal vascular rupture. The mechanisms underlying IA formation, growth and rupture are mostly unknown, and while increasing evidence suggest a genetic component of IA, identification of specific genes or causal molecular pathways remains largely inconclusive and only a small fraction of the risk attributable to genetics for IA in the general population. Methods: Here, we combined whole exome sequencing and identity-by -descent analyses with functional investigations to identify rare IA predisposing variants in familial forms of IA and understand their contribution to the pathophysiology of IA. Results: We identified two rare missense variants in the CTSO gene shared by all the affected relatives in two large pedigrees with multiple IA-affected relative. CTSO encodes for the cysteine-type papain-like cathepsin CTSO. Functional analyses revealed that CTSO acts as an extracellular protease controlling vascular smooth muscle cell migration and adhesion to the extracellular matrix. CTSO depletion, as well as expression of the two CTSO variants, which were poorly secreted, led to increase the amount of fibronectin. This effect is associated with a marked increase in VSMC stiffness which was rescued by exogenous CTSO. Conclusions: This report identifies rare CTSO variants in familial IA patients and suggests that the increased susceptibility to IA induced by these variants is likely related to their primary effects on the vascular tissue, and more particularly on the media layer of the wall of cerebral arteries.

show abstract

“…Previous studies show that higher mechanical strength might exist in hydrogels with physical crosslinking, providing new ideas for their application. 6,7 Though previous studies contributed a lot in synthesizing high-strength hydrogels or enhancing natural polymer hydrogels, the preparation of hydrogels combined with good biocompatibility and mechanical properties still faces many challenges.…”

Section: Introductionmentioning

confidence: 99%