Mechanical properties of mouse lungs along organ decellularization by sodium dodecyl sulfate

Pressure- and flow-controlled media perfusion differently modify vascular mechanics in lung decellularization

“…Dynamic (E dyn ) and static E st ) lung elastances were determined in additional organs (3 native lungs and 11 acellular lungs immediately after decellularization). To characterize the pressure-volume relationship in mechanical conditions similar to those in physiologically normal breathing, the lungs were subjected to conventional mechanical ventilation, following a procedure describe in detail elsewhere (Nonaka et al, 2014a(Nonaka et al, , 2014bUriarte et al, 2014). Briefly, the lungs were tracheally intubated, suspended vertically by gravity and placed within a chamber (32 1C and 100% humidity).…”

“…Taking into account the value of pre-inspiratory pressure (P o ), lung static elastance (E st ) was computed as the adjusted plateau pressure (P el -P o ) recorded after 5 s of occlusion divided by the tidal volume. E dyn was computed by dividing the adjusted inflection point pressure (P i -P o ) by the tidal volume (Nonaka et al, 2014a(Nonaka et al, , 2014b. For each native and decellularized lung, E st and E dyn were obtained as the means from five end-inspiratory occlusions, each one carried out after 1 min of normal mechanical ventilation.…”

Behavior of vascular resistance undergoing various pressure insufflation and perfusion on decellularized lungs

“…The decellularization process eliminates lung cells, i.e., type II alveolar epithelial cells, which secrete lung surfactant, thereby increasing the lung compliance as described previously (da Palma et al, 2015;Nonaka et al, 2014). Owing to low lung elastance, this decellularized lung can no longer increase the tension in the alveolar walls to alter vascular resistance, thereby explaining the slight influence of CPAP on vascular resistance.…”

“…As described in a previous study (Nonaka et al, 2014), pulmonary decellularization was carried out by a combination of freezing/thawing methods, and SDS removed cellular debris while preserving the mechanical properties of the structure. No significant changes in the resistance values and elastance of the lungs were observed during conventional mechanical ventilation.…”

Histological Evaluation of Decellularized Skeletal Muscle Tissue Using Two Different Decellularization Agents

“…Successful decellularization produces biological material which combines advantages and properties of extracellular matrix as supportive microarchitecture, vascular network and participate in cell differentiation and proliferation (5,6). Biological scaffolds composed of the extracellular matrix are commonly used in reconstructive surgical applications of many organs such as urinary bladder (7,8), heart (9-11), lung (12,13) or skin (14,15).…”