Pressure- and flow-controlled media perfusion differently modify vascular mechanics in lung decellularization

Palma, Renata Kelly da; Campillo, Noelia; Uriarte, Juan J.; Oliveira, Luís Vicente Franco de; Navajas, Daniel; Farré, Ramón

doi:10.1016/j.jmbbm.2015.04.024

Cited by 31 publications

(38 citation statements)

References 43 publications

(77 reference statements)

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“…The decellularization process eliminates lung cells, i.e., type II alveolar epithelial cells, which secrete lung surfactant, thereby increasing the lung compliance as described previously (da Palma et al, 2015;Nonaka et al, 2014). Owing to low lung elastance, this decellularized lung can no longer increase the tension in the alveolar walls to alter vascular resistance, thereby explaining the slight influence of CPAP on vascular resistance.…”

Section: Discussionmentioning

confidence: 91%

“…It is known that the absence of surfactant in decellularized lungs may cause the alveolar walls to collapse; therefore, in a previous study, we used a CPAP of 10 cmH 2 O to keep the lungs inflated during the decellularization process (da Palma et al, 2015). However, the influence of CPAP on vascular resistance in acellular lungs was not known.…”

Section: Discussionmentioning

confidence: 99%

“…Once the trachea and pulmonary artery were cannulated and placed into the experimental system, the trachea was connected to a continuous positive airway pressure (CPAP) device that was set to provide a tracheal (i.e., transpulmonary) pressure of 10 cmH 2 O to inflate the lung close to total lung capacity in an attempt to avoid atelectasis (da Palma et al, 2015). The following sequence of decellularizing process, the lungs were perfused through the pulmonary artery: 1) PBS 1 Â for 30 min, 2) deionized water for 15 min, 3) 1% sodium dodecyl sulfate (SDS) for 150 min and 4) PBS for 30 min, at a pressure of 20 cmH 2 O.…”

Section: Lung Decellularizationmentioning

confidence: 99%

“…Previous study from our group demonstrated that effective vascular resistance varies considerably during the process of decellularization (da Palma et al, 2015). However, data on circulatory resistance of scaffolds as a function of airway and vascular pressures are unavailable.…”

Section: Introductionmentioning

confidence: 97%

“…Monitoring vascular resistance could also be a useful quality control tool for future high-throughput production (da Palma et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Behavior of vascular resistance undergoing various pressure insufflation and perfusion on decellularized lungs

Palma

Nonaka

Campillo

et al. 2016

Journal of Biomechanics

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a b s t r a c tBioengineering of functional lung tissue by using whole lung scaffolds has been proposed as a potential alternative for patients awaiting lung transplant. Previous studies have demonstrated that vascular resistance (Rv) could be altered to optimize the process of obtaining suitable lung scaffolds. Therefore, this work was aimed at determining how lung inflation (tracheal pressure) and perfusion (pulmonary arterial pressure) affect vascular resistance. This study was carried out using the lungs excised from 5 healthy male Sprague-Dawley rats. The trachea was cannulated and connected to a continuous positive airway pressure (CPAP) device to provide a tracheal pressure ranging from 0 to 15 cmH 2 O. The pulmonary artery was cannulated and connected to a controlled perfusion system with continuous pressure (gravimetric level) ranging from 5 to 30 cmH 2 O. Effective Rv was calculated by ratio of pulmonary artery pressure (P PA ) by pulmonary artery flow (V 0 PA ). Rv in the decellularized lungs scaffolds decreased at increasing V 0 PA, stabilizing at a pulmonary arterial pressure greater than 20 cmH 2 O. On the other hand, CPAP had no influence on vascular resistance in the lung scaffolds after being subjected to pulmonary artery pressure of 5 cmH 2 O. In conclusion, compared to positive airway pressure, arterial lung pressure markedly influences the mechanics of vascular resistance in decellularized lungs.

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Lung Decellularizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

“…Monitoring vascular resistance could also be a useful quality control tool for future high-throughput production (da Palma et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Behavior of vascular resistance undergoing various pressure insufflation and perfusion on decellularized lungs

Palma

Nonaka

Campillo

et al. 2016

Journal of Biomechanics

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Comparative study of two perfusion routes with different flow in decellularization to harvest an optimal pulmonary scaffold for recellularization

Wang

et al. 2016

J Biomedical Materials Res

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Decellularization processes may variably distort or degrade extracellular matrix (ECM) structure. In this study, two perfusion routes (PR) were tested on SD rat lung samples. One decellularization protocol, PR1, was perfused through the pulmonary artery. The other decellularization protocol, PR2, was perfused through the trachea. Both decellularization protocols were used by the same detergent-based (sodium dodecyl sulphate and Triton X-100) with different flow continuous perfusion. There was no visible difference in vessel architecture between PR1- and PR2-decellularized scaffold. However, the airway structure and alveoli architecture of pulmonary decellularized scaffolds generated through PR2 at a flow rate of 8 mL/min were destroyed partly when compared to that in native lung and PR1-decellularized scaffold. Ultramicroscopic assessment of scaffolds was similar in both protocols and showed filamentous ECM with preserved fiber disposition and structure. Histological analysis and immunostaining showed no detectable cells remaining in the pulmonary scaffolds compare with native lung. The DNA concentration was significantly reduced in the decellularized scaffolds compared to the native lungs. A549 cells reseeded onto decellularized pulmonary scaffolds were no significant difference between PR1 and PR2 in cell viability, p > 0.05. We conclude that under the same high flow velocity status, perfusion decellularization through the pulmonary artery may be an optimal pathway to obtain decellularized scaffolds for pulmonary regeneration. This article is protected by copyright. All rights reserved. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2567-2575, 2016.

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Perfusion decellularization of porcine esophagus: Study of two processing factors affecting the folded mucosal structure of the esophageal scaffold

Sitthisang

Leong

Chian

2020

J Biomedical Materials Res

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Acellular scaffolds from decellularized donor organs are showing promising clinical results in tissue and organ repair and regeneration. A successful decellularization process is determined by (a) its capability to decellularize complete organs of large animals, (b) retention of the extracellular matrix (ECM) structures and morphologies, and (c) minimal loss of ECM proteins. In this study, porcine esophagi were perfused in full thickness with 0.25% w/v sodium dodecyl sulfate at perfusion rates 0.1–0.2 ml/min for up to 5 days. Decellularized tissues were characterized for their residual DNA, histological staining for their matrix structures, immunohistochemical staining for collagen type IV and laminin, and scanning electron microscopy for structural integrity. Our results showed that full thickness esophageal tissues treated using the horizontal perfusion setup were decellularized with good structural and biochemical integrity in the ECM. Residual DNA content in decellularized tissues was found to be 36 ± 12 ng/mg of tissues (n = 6) which was significantly lower than that of native tissues (p = .00022). Our study showed that the organ must be decellularized in full thickness and perfusion pressure must be controlled to minimize radial expansion. These factors were found to be critical in preserving the folded mucosa in the decellularized tissues.

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Pressure- and flow-controlled media perfusion differently modify vascular mechanics in lung decellularization

Cited by 31 publications

References 43 publications

Behavior of vascular resistance undergoing various pressure insufflation and perfusion on decellularized lungs

Behavior of vascular resistance undergoing various pressure insufflation and perfusion on decellularized lungs

Comparative study of two perfusion routes with different flow in decellularization to harvest an optimal pulmonary scaffold for recellularization

Perfusion decellularization of porcine esophagus: Study of two processing factors affecting the folded mucosal structure of the esophageal scaffold

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