Mechanical properties of collagen gels derived from rats of different ages

Wu, Ching-Chou; Ding, Shinn Jyh; Wang, Yao Hsien; Tang, Ming Jer; Chang, Hsien Chang

doi:10.1163/156856205774269494

Cited by 54 publications

(38 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…21 Plastic compression therefore yields scaffolds much denser than conventional, uncompressed collagen type I matrices. The moduli of these conventional scaffolds are typically below 1 kPa, 22,23 and these scaffolds should first grow stronger in culture. 21 Our technique of plastic compression differs from earlier studies, because we use cell culture inserts with 3 mm pores to reduce the liquid content instead of nylon and stainless steel meshes.…”

Section: Discussionmentioning

confidence: 99%

Rheological characterization of the nucleus pulposus and dense collagen scaffolds intended for functional replacement

Bron

Koenderink

Everts

et al. 2008

Journal Orthopaedic Research

View full text Add to dashboard Cite

Lumbar discectomy is an effective therapy for neurological decompression in patients suffering from sciatica due to a herniated nucleus pulposus (NP). However, high numbers of patients suffering from persisting postoperative low back pain have resulted in many strategies targeting the regeneration of the NP. For successful regeneration, the stiffness of scaffolds is increasingly recognized as a potent mechanical cue for the differentiation and biosynthetic response of (stem) cells. The aim of the current study is to characterize the viscoelastic properties of the NP and to develop dense collagen scaffolds with similar properties. The scaffolds consisted of highly dense (0.5%-12%) type I collagen matrices, prepared by plastic compression. The complex modulus of the NP was 22 kPa (at 10 rad s À1 ), which should agree with a scaffold with a collagen concentration of 23%. The loss tangent, indicative of energy dissipation, is higher for the NP (0.28) than for the scaffolds (0.12) and was not dependent of the collagen density. Gamma sterilization of the scaffolds increased the shear moduli but also resulted in more brittle behavior and a reduced swelling capacity. In conclusion, by tuning the collagen density, we can approach the stiffness of the NP. Therefore, dense collagen is a promising candidate for tissue engineering of the NP that deserves further study, such as the addition of other proteins. ß

show abstract

Section: Discussionmentioning

confidence: 99%

Rheological characterization of the nucleus pulposus and dense collagen scaffolds intended for functional replacement

Bron

Koenderink

Everts

et al. 2008

Journal Orthopaedic Research

View full text Add to dashboard Cite

show abstract

“…Interestingly, although immunofluorescence detection for Type-I and Type-III collagen did not show substantial differences at the protein level between young and old prostate tissue, confocal microscopy revealed a disorganized collagen matrix in the aged prostate microenvironment when compared to young prostate (Figure 6b). Previous studies have demonstrated the effect of fibrillar collagen and substrate rigidity on epithelial cell proliferation, apoptosis, and motility in culture systems, suggesting a direct role of the collagen network on the behavior of epithelial cells (8)(9)(10). Taken together, we hypothesized that the disorganized collagen matrix and the diminished expression of Col1a2 and Col3a1 in the aged prostate microenvironment may have an effect on the structural and signaling properties of the extracellular matrix.…”

Section: Bodymentioning

confidence: 74%

The Aged Microenvironment Influences Prostate Carcinogenesis

Bianchi-Frias¹

2009

View full text Add to dashboard Cite

Public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing this collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden to Department of Defense, Washington Headquarters Services, Directorate for Information Operations and Reports (0704-0188), 1215 Jefferson Davis Highway, Suite 1204, Arlington, VA 22202-4302. Respondents should be aware that notwithstanding any other provision of law, no person shall be subject to any penalty for failing to comply with a collection of information if it does not display a currently valid OMB control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS. REPORT DATE PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) 8. PERFORMING ORGANIZATION REPORT NUMBERFred Hutchinson Cancer Research Center Seattle, WA 98109 SPONSORING / MONITORING AGENCY NAME(S) AND ADDRESS(ES) 10. SPONSOR/MONITOR'S ACRONYM(S) U.S. Army Medical Research and Materiel Command Fort Detrick, Maryland 21702-5012 SPONSOR/MONITOR'S REPORT NUMBER(S) DISTRIBUTION / AVAILABILITY STATEMENTApproved for Public Release; Distribution Unlimited SUPPLEMENTARY NOTES ABSTRACTThe greatest factor for the development of prostate adenocarcinoma is advanced age. Emerging evidence suggests that molecular alterations in the aged prostate microenvironment mediated by stromal aging and senescence are key factors regulating carcinogenesis and neoplastic progression. We used normal mouse prostate epithelial and adjacent stromal cells from young and old animals, microdissected in situ, to identify factors altered by the aged stroma that may place the prostate gland at risk for developing prostate cancer. Expression profiling demonstrated clear differences in gene expression between old and young prostate stroma, with 219 genes exhibiting significant transcript abundance levels (p<0.005). Transcripts for Type I and III collagen were among the genes most substantially altered with aging. qRT-PCR confirmed the lower expression of Col1a2 and Col3a1 in the aged prostate stroma and immunofluorescence detection for Type-I collagen revealed a disorganized collagen matrix in the aged prostate. The alterations in the collagen network affect the structural and signaling properties of the extracellular matrix and in turn could plausibly facilitate carcinogenesis and neoplastic progression. The aged-expression profiling also demonstrated the up-regulation of several chemokines (Ccl8, Ccl7), and factors that respond to pro-inflammatory agonist and senescent inducers, such as ApoD in the aged stroma. In summary, the transcriptional profile of the aged prostate microenvironment provides novel data regarding senescence-associated candidate genes important in prostate carcinogenesis. Further studies are necessary in order to provide a functiona...

show abstract

“…Billiar (2011) report testing AFM for this purpose, but they could not measure differences between polymerized and nonpolymerized collagen. A surface tension-induced artefact is mentioned in a comment from Wu et al (2005) for application of dynamic mechanical analysis of hydrated collagen gels. Our own observations allow the same conclusion.…”

Section: Determining the Elastic Modulus Using Afmmentioning

confidence: 99%

“…• C. To avoid bias by surface tension at the hydrogel to air interface (Wu et al, 2005), all measurements were conducted with the cantilevers submerged in preheated phosphate-buffered saline (PBS). Force spectroscopy measurements were conducted using silicon-nitride cantilevers (CSC38, Mikromasch) with force constants ranging from 0.03 to 0.08 N m −1 and quasi-conical tips with an opening angle between 34…”

Section: Determining the Elastic Modulus Using Afmmentioning

confidence: 99%

Quantification of substrate and cellular strains in stretchable 3D cell cultures: an experimental and computational framework

Gonzalez-Avalos

Mürnseer

Deeg

et al. 2017

Journal of Microscopy

View full text Add to dashboard Cite

SummaryThe mechanical cell environment is a key regulator of biological processes . In living tissues, cells are embedded into the 3D extracellular matrix and permanently exposed to mechanical forces. Quantification of the cellular strain state in a 3D matrix is therefore the first step towards understanding how physical cues determine single cell and multicellular behaviour. The majority of cell assays are, however, based on 2D cell cultures that lack many essential features of the in vivo cellular environment. Furthermore, nondestructive measurement of substrate and cellular mechanics requires appropriate computational tools for microscopic image analysis and interpretation. Here, we present an experimental and computational framework for generation and quantification of the cellular strain state in 3D cell cultures using a combination of 3D substrate stretcher, multichannel microscopic imaging and computational image analysis. The 3D substrate stretcher enables deformation of living cells embedded in bead-labelled 3D collagen hydrogels. Local substrate and cell deformations are determined by tracking displacement of fluorescent beads with subsequent finite element interpolation of cell strains over a tetrahedral tessellation. In this feasibility study, we debate diverse aspects of deformable 3D culture construction, ¶ These two authors contribute equally.

show abstract

Mechanical properties of collagen gels derived from rats of different ages

Cited by 54 publications

References 24 publications

Rheological characterization of the nucleus pulposus and dense collagen scaffolds intended for functional replacement

Rheological characterization of the nucleus pulposus and dense collagen scaffolds intended for functional replacement

The Aged Microenvironment Influences Prostate Carcinogenesis

Quantification of substrate and cellular strains in stretchable 3D cell cultures: an experimental and computational framework

Contact Info

Product

Resources

About