Mechanical properties of breast, kidney, and thyroid tumours measured by AFM: Relationship with tissue structure

Levillain, Aurélie; Confavreux, Cyrille; Decaussin‐Petrucci, Myriam; Durieux, E.; Paparel, P.; Carval, K Lebail; Maillard, L.; Bermond, François; Mitton, David; Follet, Hélène

doi:10.1016/j.mtla.2022.101555

Cited by 5 publications

(6 citation statements)

References 56 publications

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“…Except for P(CL-co-DLLA), the mechanical properties achieved using hydrogel models fell within the range of those found in actual tumor samples. However, the values can significantly differ based on the cancer type, sample type, and methodology employed to measure mechanical properties [140][141][142][143][144].…”

Section: Mechanical Characterization Of the Hydrogelsmentioning

confidence: 99%

“…As mentioned earlier and demonstrated in table 4, most in vitro studies use oscillatory rheometry to characterize samples. However, when transitioning to ex vivo studies that measure the viscoelastic properties of various tumors, the most frequently used techniques are based on nanoindentation [7,8,141,143,174], with oscillatory rheometry and compression tests being the exception rather than the rule [117,144]. The ability to translate observations made in vitro to ex vivo and vice versa will be crucial in the future to validate the relationship between viscoelasticity and cancer progression.…”

Section: Concluding Remarks and Future Perspectivesmentioning

confidence: 99%

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Beyond stiffness: deciphering the role of viscoelasticity in cancer evolution and treatment response

Zubiarrain-Laserna,

Martínez-Moreno,

López de Andrés

et al. 2024

Biofabrication

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There is increasing evidence that cancer progression is linked to tissue viscoelasticity, which challenges the commonly accepted notion that stiffness is the main mechanical hallmark of cancer. However, this new insight has not reached widespread clinical use, as most clinical trials focus on the application of tissue elasticity and stiffness in diagnostic, therapeutic, and surgical planning. Therefore, there is a need to advance the fundamental understanding of the effect of viscoelasticity on cancer progression, to develop novel mechanical biomarkers of clinical significance. Tissue viscoelasticity is largely determined by the extracellular matrix (ECM), which can be simulated in vitro using hydrogel-based platforms. Since the mechanical properties of hydrogels can be easily adjusted by changing parameters such as molecular weight and crosslinking type, they provide a platform to systematically study the relationship between ECM viscoelasticity and cancer progression. This review begins with an overview of cancer viscoelasticity, describing how tumor cells interact with biophysical signals in their environment, how they contribute to tumor viscoelasticity, and how this translates into cancer progression. Next, an overview of clinical trials focused on measuring biomechanical properties of tumors is presented, highlighting the biomechanical properties utilized for cancer diagnosis and monitoring. Finally, this review examines the use of biofabricated tumor models for studying the impact of ECM viscoelasticity on cancer behavior and progression and it explores potential avenues for future research on the production of more sophisticated and biomimetic tumor models, as well as their mechanical evaluation.

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Section: Mechanical Characterization Of the Hydrogelsmentioning

confidence: 99%

Section: Concluding Remarks and Future Perspectivesmentioning

confidence: 99%

Beyond stiffness: deciphering the role of viscoelasticity in cancer evolution and treatment response

Zubiarrain-Laserna,

Martínez-Moreno,

López de Andrés

et al. 2024

Biofabrication

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“…Hertzian (for spherical indenters) or Sneddon (for conical indenters) contact mechanics models (Equations (1) and (2), respectively) are commonly used to fit force–distance curves and estimate the Young’s modulus of biological samples:

where F is the applied load; E is the Young’s modulus; R is the tip radius;

is the Poisson’s ratio;

is the indentation depth; and

is the cone half angle. In cases in which the indenter is modified (e.g., a microsphere glued to the free end of a rectangular tip) and/or has a non-spherical and non-conical geometry (e.g., pyramidal), other models are used [ 30 , 31 , 32 , 33 , 34 ]. For example, the equation to analyze curves gathered by a four-sided, blunted pyramidal tip is as follows:

…”

Section: Principles Of Atomic Force Microscopy For Studying Tissue Me...mentioning

confidence: 99%

“…Indeed, viscous forces are important in other contexts as well. One study found that while the elastic modulus alone is able to differentiate between breast, kidney, and thyroid cancer subtypes, viscosity is able to discriminate between normal and malignant thyroid tissue [ 30 ]. Here, the elastic modulus and viscosity are quantified using Equation (3) and the creep function for a standard linear solid viscoelastic model, respectively.…”

Section: Application Of Atomic Force Microscopy To Study Cancer Patho...mentioning

confidence: 99%

“…where F is the applied load; E is the Young's modulus; R is the tip radius; ν is the Poisson's ratio; δ is the indentation depth; and α is the cone half angle. In cases in which the indenter is modified (e.g., a microsphere glued to the free end of a rectangular tip) and/or has a non-spherical and non-conical geometry (e.g., pyramidal), other models are used [30][31][32][33][34].…”

Section: Modelsmentioning

confidence: 99%

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Atomic Force Microscopy Methods to Measure Tumor Mechanical Properties

Najera

Rosenberger

Datta

2023

Cancers

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Atomic force microscopy (AFM) is a popular tool for evaluating the mechanical properties of biological materials (cells and tissues) at high resolution. This technique has become particularly attractive to cancer researchers seeking to bridge the gap between mechanobiology and cancer initiation, progression, and treatment resistance. The majority of AFM studies thus far have been extensively focused on the nanomechanical characterization of cells. However, these approaches fail to capture the complex and heterogeneous nature of a tumor and its host organ. Over the past decade, efforts have been made to characterize the mechanical properties of tumors and tumor-bearing tissues using AFM. This has led to novel insights regarding cancer mechanopathology at the tissue scale. In this Review, we first explain the principles of AFM nanoindentation for the general study of tissue mechanics. We next discuss key considerations when using this technique and preparing tissue samples for analysis. We then examine AFM application in characterizing the mechanical properties of cancer tissues. Finally, we provide an outlook on AFM in the field of cancer mechanobiology and its application in the clinic.

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Raman-AFM-fluorescence-guided impact of linoleic and eicosapentaenoic acids on subcellular structure and chemical composition of normal and cancer human colon cells

Beton-Mysur,

Surmacki,

Brożek-Płuska

2024

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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Mechanical properties of breast, kidney, and thyroid tumours measured by AFM: Relationship with tissue structure

Cited by 5 publications

References 56 publications

Beyond stiffness: deciphering the role of viscoelasticity in cancer evolution and treatment response

Beyond stiffness: deciphering the role of viscoelasticity in cancer evolution and treatment response

Atomic Force Microscopy Methods to Measure Tumor Mechanical Properties

Raman-AFM-fluorescence-guided impact of linoleic and eicosapentaenoic acids on subcellular structure and chemical composition of normal and cancer human colon cells

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