Mechanical properties of acellular mouse lungs after sterilization by gamma irradiation

Pressure- and flow-controlled media perfusion differently modify vascular mechanics in lung decellularization

“…The lung, however, has an additional possible route for decellularization since the media can be also infused through the trachea. In fact, both routes (trachea and pulmonary artery) alone or combined have been used to effectively decellularize lungs (Girard et al, 2013;Nonaka et al, 2014aNonaka et al, , 2014bUriarte et al, 2014;Melo et al 2014;Wagner et al, 2014aWagner et al, , 2014bKhalpey et al, 2014;Price et al, 2014). This study was focused on infusing decellularization agents through the vascular bed since is the most conventional route in lungs and the only available in other relevant organs.…”

“…Dynamic (E dyn ) and static E st ) lung elastances were determined in additional organs (3 native lungs and 11 acellular lungs immediately after decellularization). To characterize the pressure-volume relationship in mechanical conditions similar to those in physiologically normal breathing, the lungs were subjected to conventional mechanical ventilation, following a procedure describe in detail elsewhere (Nonaka et al, 2014a(Nonaka et al, , 2014bUriarte et al, 2014). Briefly, the lungs were tracheally intubated, suspended vertically by gravity and placed within a chamber (32 1C and 100% humidity).…”

Methods of tissue decellularization used for preparation of biologic scaffolds and in vivo relevance

“…It is noteworthy that the measurement of 137 decellularization efficacy used in each study was unique and com-138 mon criteria were not used. Decellularization efficacy is discussed 139 in more detail in a later section. Undoubtedly, the resultant eso- 140 phageal ECM for each protocol was distinct and unique.…”

Coming to terms with tissue engineering and regenerative medicine in the lung

“…Added to these passive forces are the active mechanical forces imposed on both airways and vasculature by luminal contraction and relaxation in response to changing demands, and by endogenous and exogenous factors such as local and circulating agonists and antagonists. Although in silico models (79,83,99,132,148,196), in vitro and ex vivo systems (59,92,103,108,118,176,193,202,203), and whole-organ approaches (94,143,205,206) have been developed to explore the importance of such mechanical forces, further advances in airway, lung parenchyma, and vascular mechanobiology will be critical for bioengineering a lung capable of withstanding the internal and external forces exerted on the transplanted lung within the chest cavity and will be critical to ensuring that lung function occurs without airway collapse, injury, or failure of gas exchange. The challenges to our current understanding of lung mechanobiology and their implications for a bioengineered lung are discussed in Materials, Matrix, and Mechanobiology.…”