Mechanical properties of a laminin–apatite composite layer formed on an ethylene–vinyl alcohol copolymer

Oyane, Ayako; Yokoyama, Yoshiro; Kasahara, Mayumi; Ichinose, Noboru; Saitô, Makoto; Ito, Atsuo

doi:10.1016/j.msec.2009.01.012

Cited by 14 publications

(18 citation statements)

References 30 publications

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“…The HA nuclei grow into flakes, merged, and finally formed a continuous and homogeneous layer. During the spontaneous growth of HA crystals on the HA disk and HA scaffold surfaces, plasmid and lipid (FuGENE®), in the form of either discrete molecule or complex, are adsorbed onto the growing crystal surface through electrostatic interaction, taking into account our previous reports on protein-apatite composite layers [25][26][27][28][29][30][31][32][33]. The obviously decreased HA crystal size of the DLp-Ap layer (Fig.…”

Section: Discussionmentioning

confidence: 98%

DNA-lipid-apatite composite layers enhance gene expression of mesenchymal stem cells

Wang

Ito

et al. 2013

Materials Science and Engineering: C

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Section: Discussionmentioning

confidence: 98%

DNA-lipid-apatite composite layers enhance gene expression of mesenchymal stem cells

Wang

Ito

et al. 2013

Materials Science and Engineering: C

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“…The great advantage of our coating process is the immobilization of biomolecules (such as proteins, [20][21][22][23] antibiotics, 5 and DNA [24][25][26] ), which retain their biological activity, into the HA layer on polymer specimens. In the present study, laminin, [27][28][29] which is a major cell adhesion protein, was immobilized into the HA layer to increase the shear strength of the layer 30 and promote cell adhesion onto the layer, 20,31 which would facilitate implant-skin integration.…”

Section: Contract Grant Sponsors: Industrial Technology Research Granmentioning

confidence: 99%

“…30 This physical reinforcement by the immobilized laminin is likely to be responsible for the improved adhesion between the EVLCP specimen and the skin tissue ( Figure 11). Differences in layer solubility and thickness between the HA and LHA layers, which arise from an impurity effect on the layer composition and an inhibitory effect on the HA growth of the laminin supplements, 21 could also be possible factors that affect the adhesion with skin tissue.…”

Section: Biocompatibility Of the Ha Coating Layermentioning

confidence: 99%

Preliminary in vivo study of apatite and laminin‐apatite composite layers on polymeric percutaneous implants

et al. 2011

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A polymeric material coated with a hydroxyapatite (HA) layer would be useful as a flexible percutaneous device with good biocompatibility and resistance to bacterial infection. We have recently developed a simple, safe, and mild coating process to form an HA layer on the surfaces of polymeric materials. In this study, our coating process was applied to an ethylene-vinyl alcohol copolymer film. The resulting HA-coated film was percutaneously implanted in the scalp of a rat to examine the stability and biocompatibility of the HA layer. From the results of histological analysis, the HA layer remained undissolved on the film surface under the skin tissue even 3 days after implantation. Owing to the good biocompatibility of HA, the HA-coated film suppressed a host's foreign-body response and integrated with the surrounding skin tissue for as long as 14 days, in a similar fashion to a conventional percutaneous device composed of ceramic HA. Immobilization of a cell adhesion protein, laminin, into the HA layer was found to improve the adhesion strength between the film and the surrounding skin tissue without compromising good biocompatibility of HA. Our coating process to form HA and laminin-HA composite layers would be useful in fabricating polymeric percutaneous devices with a reduced risk of bacterial infection, although further in vivo studies are required.

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“…34) As determined by a mechanical test, the laminin molecules immobilized in the LAp layer increased the layer's shear strength under wet condition, probably owing to electrostatic interactions with the surrounding apatite crystals. 36) As determined by an in vitro test, the laminin molecules retained their inherent cell adhesion property even in the LAp layer; they apparently enhanced adhesion and spreading of various cells on the layer (Fig. 5). 30),31),45),47) Such a biological effect of laminin …”

Section: Biomimetic Process For Apatite Coatingmentioning

confidence: 84%

Development of apatite-based composites by a biomimetic process for biomedical applications

Oyane

2010

J. Ceram. Soc. Japan

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A new biomimetic process for apatite coating on polymeric materials has been developed. In this process, the surface of a polymer is modified with amorphous calcium phosphate (ACP), and then the polymer is immersed in a supersaturated calcium phosphate solution. The new biomimetic process has the advantages of safety, simplicity, and applicability to various types and forms of polymeric materials. By adding a biomolecule (such as protein, antibacterial agent, or DNA) to the supersaturated solution, immobilization of a biomolecule into the apatite coating while retaining the intrinsic biological activity of the biomolecule is possible. As a result of this, the base polymer would possess biological activity to control cell behaviors (such as adhesion, proliferation, and differentiation), in addition to good biocompatibility owing to the apatite. Hence, the new biomimetic process and the resulting composites have a wide variety of biomedical applications including tissue engineering scaffolds, percutaneous devices, and gene delivery carriers.

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Mechanical properties of a laminin–apatite composite layer formed on an ethylene–vinyl alcohol copolymer

Cited by 14 publications

References 30 publications

DNA-lipid-apatite composite layers enhance gene expression of mesenchymal stem cells

DNA-lipid-apatite composite layers enhance gene expression of mesenchymal stem cells

Preliminary in vivo study of apatite and laminin‐apatite composite layers on polymeric percutaneous implants

Development of apatite-based composites by a biomimetic process for biomedical applications

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