Mechanical force augments the anti‐osteoclastogenic potential of human gingival fibroblasts <i>in vitro</i>

Sh, Kook; Son, Young‐Ok; Choe, Yeong Hun; Kim, Jeehwan; Ym, Jeon; Js, Heo; Jc, Lee

doi:10.1111/j.1600-0765.2008.01121.x

Cited by 21 publications

(16 citation statements)

References 29 publications

(48 reference statements)

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“…It was recently reported that mechanical stress loading affects osteoblasts and periodontal ligament cells to regulate inflammatory cytokine expression (Yamamoto et al , 2006; Maeda et al , 2007; Koyama et al , 2008; Kook et al , 2009), receptor activator of nuclear factor κB ligand (RANKL), and osteoprotegerin (OPG) (Tang et al , 2006; Kreja et al , 2008; Nakajima et al , 2008). We have also reported that mechanical stress as hydrostatic pressure induced cytokine production in human periodontal ligament cells (Yamamoto et al , 2006, 2011).…”

Section: Introductionmentioning

confidence: 99%

Effects of mechanical stress on cytokine production in mandible‐derived osteoblasts

Yamamoto

Ichioka

et al. 2011

Oral Diseases

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Section: Introductionmentioning

confidence: 99%

Effects of mechanical stress on cytokine production in mandible‐derived osteoblasts

Yamamoto

Ichioka

et al. 2011

Oral Diseases

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“…While embryo-like gingival healing is characterized by the absence of fibrosis or irreversible elastin/collagen degradation, the paracrine effect of gingival fibroblasts results in the preservation of elastic fiber and decrease in MMP-1, -3, and -9 production contributing to diminish the degradation of artery when cocultured with human aortic smooth muscle cells (Naveau et al, 2007). Mechanical stimulation up-regulates bone-related protein expression and accelerates osteoprotegerin production by gingival fibroblasts to suppress osteoclastogenesis (Kook et al, 2009). Mechanical stimulation up-regulates bone-related protein expression and accelerates osteoprotegerin production by gingival fibroblasts to suppress osteoclastogenesis (Kook et al, 2009).…”

mentioning

confidence: 99%

“…A graft composed of fibronectin matrixbased multilayered cell sheets of human gingival fibroblasts modified to express alkaline phosphatase (ALP) supported alveolar bone regeneration and exhibited potential for periodontal tissue engineering applications (Nakajima et al, 2008). Mechanical stimulation up-regulates bone-related protein expression and accelerates osteoprotegerin production by gingival fibroblasts to suppress osteoclastogenesis (Kook et al, 2009). These findings hint that human gingival fibroblasts have unique features and ability of undergoing induction-directed differentiation and regeneration.…”

mentioning

confidence: 99%

Characterization of mesenchymal stem cells from human normal and hyperplastic gingiva

Tang

Xie

et al. 2010

Journal Cellular Physiology

151

155

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Human gingiva plays an important role in the maintenance of oral health and shows unique fetal-like scarless healing process after wounding. Here we isolate and characterize mesenchymal stem cells from human normal and hyperplastic gingival tissues (N-GMSC and H-GMSC, respectively). Immunocytochemical staining indicated that gingival lamina propria contained Stro-1 and SSEA-4 positive cells, implying existence of putative gingival MSC. Under attachment-based isolating and culturing condition, gingival MSC displayed highly clonogenic and long-term proliferative capability. By using single colony isolation and expansion approaches, we found both N-GMSC and H-GMSC possessed self-renewal and multipotent differentiation properties. N-GMSC and H-GMSC showed distinct immunoregulatory functions in a murine skin allograft setting via up-regulation of putative systemic regulatory T cells (Tregs). N-GMSC and H-GMSC were capable of regenerating collagenous tissue following in vivo transplantation, in which H-GMSC exhibited more robust regenerative capability. These findings suggest that gingival tissue contains tissue-specific mesenchymal stem cell population and is an ideal resource for immunoregulatory therapy due to its substantial availability and accessibility. In addition, gingival MSC over-activation may contribute to gingival hyperplastic phenotype.

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“…These cells were used in order to determine the anti-tumor activity of the barley extracts in vitro. In addition, human gingival fibroblasts (HGF) were cultured according to previously described methods (Kook et al, 2009) and the primary cultures were used as a control for these cancer cells.…”

Section: Extraction and Fractionation Of Barley Methanol Extractsmentioning

confidence: 99%

Methanol extract of the aerial parts of barley (Hordeum vulgare) suppresses lipopolysaccharide-induced inflammatory responsesin vitroandin vivo

Choi

Hwang

Bang

et al. 2013

Pharmaceutical Biology

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Context: Recently, there has been renewed interest in barley (Hordeum vulgare L. Poaceae) as a functional food and for its medicinal properties. Objective: This study examines the anti-inflammatory potential of the active fractions of barley and the mechanisms involved. Materials and methods: The macrophages were exposed to 100 mg/mL of each of the barley extracts in the presence of 1 mg/mL lipopolysaccharide (LPS) and after 24 or 48 h of incubation, cells or culture supernatants were analyzed by various assays. The anti-inflammatory potential of barley fractions was also investigated using the LPS-injected septic mouse model. The active constituents in the fractions were identified using gas chromatography-mass spectrometry (GC-MS). Results: The active fractions, named F 4 , F 7 , F 9 and F 12 , inhibited almost completely the LPS-induced production of nitric oxide (NO) and inducible NO synthase. Pre-treatment with these fractions at 100 mg/mL diminished the tumor necrosis factor-a (TNF-a) levels to 19.8, 3.5, 1.2 and 1.7 ng/mL, respectively, compared to LPS treatment alone (41.5 ng/mL). These fractions at 100 mg/mL also suppressed apparently the secretion of interleukin (IL)-6 and IL-1b and the DNA-binding activity of nuclear factor-kB in LPS-stimulated cells. Mice injected intraperitoneally with LPS (30 mg/kg BW) showed 20% survival at 48 h after injection, whereas oral administration of the fractions improved the survival rates to 80%. GC-MS analysis revealed the presence of the derivatives of benzoic and cinnamic acids and fatty acids in the fractions. Discussion and conclusion: The aerial parts of barley are useful as functional food to prevent acute inflammatory responses.

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Mechanical force augments the anti‐osteoclastogenic potential of human gingival fibroblasts in vitro

Cited by 21 publications

References 29 publications

Effects of mechanical stress on cytokine production in mandible‐derived osteoblasts

Effects of mechanical stress on cytokine production in mandible‐derived osteoblasts

Characterization of mesenchymal stem cells from human normal and hyperplastic gingiva

Methanol extract of the aerial parts of barley (Hordeum vulgare) suppresses lipopolysaccharide-induced inflammatory responsesin vitroandin vivo

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