Mechanical characterization of cancer cells during TGF-β1-induced epithelial-mesenchymal transition using an electrodeformation-based microchip

Teng, Yun; Pang, Mingshu; Huang, Jianyong; Xiong, Chunyang

doi:10.1016/j.snb.2016.08.104

Cited by 23 publications

(11 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As mentioned in the preceding section, the compliance function of cells, i.e., J ( t ), can be written as the ratio of the apparent stretch strain of single cells ε( t ) to the corresponding stretch stress σ. In general, the stretch stress was independent of the cell size in the experiment. , Subsequently, we employed a simple finite-element simulation to check the influence of the initial radius of the cell involved on the apparent stretch strain. Figure A presents the geometric model we established for the electrodeformation analysis, whereas Figure B shows the spatial distribution of electrical field where a distinct electrical potential, e.g., 5 or 10 V pp , was exerted on the two electrodes.…”

Section: Resultsmentioning

confidence: 99%

“…Although the deformability of single cells was studied using deformation cytometry with the aid of microfluidic chips, , these mechanical indexes, together with the deformability, can provide more information about mechanical phenotypes of cells. Recent studies have indicated that cellular elasticity and viscosity are also key indicators of the metastatic phenotype of some kinds of cells. , The epithelial–mesenchymal transition (EMT) is often accompanied by a decrease in cellular elasticity and viscosity, which likely facilitated the migration and invasion of cells. In fact, it has already been found that the cell cycle is related to metastatic phenotypes, as revealed by prior research; ,, it was shown to be much easier to induce the metastatic phenotype at the G1/G0 phase.…”

Section: Resultsmentioning

confidence: 99%

“…Theoretically, there was a dielectrophoretic (DEP) force on a cell between two electrodes in the electrodeformation-based biomechanical microchip, which therefore produced a mechanical stimulus to deform the cell. , Generally, the DEP force was closely associated with the complex Clausius–Mosotti factor that remained nearly invariable when the frequency of electrical fields applied ranged from ∼10 5 to ∼10 7 Hz (see the Supporting Information for details). ,− This phenomenon implies that the DEP force could be viewed as a quantity independent of the frequency within the specific frequency range. In this context, the quasi-electrostatic regime could be reasonably employed to investigate the current electrodeformation of cells. , In general, the cell membrane behaved as a capacitor under the action of external electrical field, which thus gave rise to a transmembrane potential dependent on membrane geometry. , The electrical potential usually satisfied the following Laplace equation: in which Φ denotes the electrical potential, while E 0 is the electrical field. The resultant mechanical stress (i.e., force per unit area) could be solved through the classical Maxwell stress tensor: where ε is the permittivity involved and I indicates the unit tensor. ,, A finite element model was created to solve the Laplace equation and study the electrodeformation of cells based on the modules of electrostatic and solid mechanics in the Comsol Multiphysics software (COMSOL Inc., Sweden; see the Supporting Information for more details).…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Electrodeformation-Based Biomechanical Chip for Quantifying Global Viscoelasticity of Cancer Cells Regulated by Cell Cycle

et al. 2018

Self Cite

View full text Add to dashboard Cite

Mechanical phenotypes of cells are found to hold vital clues to reveal cellular functions and behaviors, which not only has great physiological significance but also is crucial for disease diagnosis. To this end, we developed a set of electrodeformation-based biomechanical microchip assays to quantify mechanical phenotypes on the single-cell level. By investigating the spatiotemporal dynamics of cancer cells driven by dielectrophoresis forces, we captured the key global viscoelastic indexes including cellular elasticity, viscosity, and transition time that was defined as the ratio of the transient viscosity and elasticity, simultaneously, and thus explored their intrinsic correlation with cell cycle progression. Our results showed that both global elasticity and viscosity have a significant periodic variation with cell cycle progression, but the transition time remained unchanged in the process, indicating that it might be an intrinsic property of cancer cells that is independent of the cell cycle and the type of cell in the experiments. Further, we investigated the molecular mechanism regulating cellular viscoelastic phenotypes on the biomechanical chips through intracellular cytoskeletal perturbation assays. These findings, together with the electrodeformation-based microchip technique, not only reveal the relation between mechanical phenotypes of cancer cells and cell cycle progression but also provide a platform for implementing multi-index mechanical phenotype assays associated with cancer cell cycles in the clinic.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Electrodeformation-Based Biomechanical Chip for Quantifying Global Viscoelasticity of Cancer Cells Regulated by Cell Cycle

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…Depending on the electric field strength and conductivity of the suspension environment, dielectrophoretic forces result in the deformation of cells (21). Many studies have resorted to AC electrodeformation to measure apparent stiffness of red blood cells and platelets (22,23), viscoelasticity of cancer cells (24,25), and to study the effect of actin depolymerization on the relaxation of electrodeformed cells (26).…”

Section: Introductionmentioning

confidence: 99%

Differential regulation of GUV mechanics via actin network architectures

Wubshet

Veerapaneni³

et al. 2022

Preprint

View full text Add to dashboard Cite

Actin networks polymerize and depolymerize to construct highly organized structures, thereby, endowing the mechanical phenotypes found in a cell. It is generally believed that the amount of filamentous actin and actin network architecture determine cytoplasmic viscosity and elasticity of the whole cell. However, the intrinsic complexity of a cell and numerous other endogenous cellular components make it difficult to study the differential role of distinct actin networks in regulating cell mechanics. Here, we model a cell by using giant unilamellar vesicles (GUVs) encapsulating actin filaments and networks assembled by various actin crosslinker proteins. Perturbation of these cytoskeletal vesicles using AC electric fields revealed that deformability depends on lumenal viscosity and actin network architecture. While actin-free vesicles exhibited large electromechanical deformations, deformations of GUVs encapsulating actin filaments were significantly dampened. The suppression of electrodeformation of actin-GUVs can be similarly recapitulated by using aqueous PEG 8000 solutions at different concentrations to modulate viscosity. Furthermore, alpha actinin-crosslinked actin networks resulted in decreased GUV deformability in comparison to actin filament-encapsulating GUVs, and membrane-associated actin networks through the formation of dendritic actin cortex greatly dampened electrodeformation of GUVs. These results highlight the organization of actin networks regulates the mechanics of GUVs and shed insights into the origin of differential deformability of cells.

show abstract

“…The intrinsic properties of cells, such as the geometrical parameters [1,2], refractive index [3,4], stiffness [5], Young modulus [6], and dielectric parameters [7], have received widespread attention in bio-related fields. More recently, the degradation of cellular mechanics and morphology has been investigated to predict the biological age of cells and ultimately reveal the ageing process and chronic disease states of older adults [8].…”

Section: Introductionmentioning

confidence: 99%

Determination of Dielectric Properties of Cells using AC Electrokinetic-based Microfluidic Platform: A Review of Recent Advances

Yang

Wang

et al. 2020

Micromachines

View full text Add to dashboard Cite

Cell dielectric properties, a type of intrinsic property of cells, can be used as electrophysiological biomarkers that offer a label-free way to characterize cell phenotypes and states, purify clinical samples, and identify target cancer cells. Here, we present a review of the determination of cell dielectric properties using alternating current (AC) electrokinetic-based microfluidic mechanisms, including electro-rotation (ROT) and dielectrophoresis (DEP). The review covers theoretically how ROT and DEP work to extract cell dielectric properties. We also dive into the details of differently structured ROT chips, followed by a discussion on the determination of cell dielectric properties and the use of these properties in bio-related applications. Additionally, the review offers a look at the future challenges facing the AC electrokinetic-based microfluidic platform in terms of acquiring cell dielectric parameters. Our conclusion is that this platform will bring biomedical and bioengineering sciences to the next level and ultimately achieve the shift from lab-oriented research to real-world applications.

show abstract

Mechanical characterization of cancer cells during TGF-β1-induced epithelial-mesenchymal transition using an electrodeformation-based microchip

Cited by 23 publications

References 64 publications

Electrodeformation-Based Biomechanical Chip for Quantifying Global Viscoelasticity of Cancer Cells Regulated by Cell Cycle

Electrodeformation-Based Biomechanical Chip for Quantifying Global Viscoelasticity of Cancer Cells Regulated by Cell Cycle

Differential regulation of GUV mechanics via actin network architectures

Determination of Dielectric Properties of Cells using AC Electrokinetic-based Microfluidic Platform: A Review of Recent Advances

Contact Info

Product

Resources

About