Mechanical characteristics of an Ormocomp® biocompatible hybrid photopolymer

Schizas, Charalampos; Karalekas, D.

doi:10.1016/j.jmbbm.2010.09.010

Cited by 43 publications

(41 citation statements)

References 27 publications

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“…Self-decontaminating surfaces would represent additional safety measure towards preventing transmission in healthcare settings. In recent years a growing interest is seen in the use of organic-inorganic hybrid materials in medicine, for example antibacterial protective coatings applied on number of devices used in healthcare [ 3 – 7 ], micro and/or nano needles for transdermal drug delivery [ 8 ], antibacterial protective coatings on the surface of artificial joints [ 9 ], or dental implants [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Protective hybrid coating containing silver, copper and zinc cations effective against human immunodeficiency virus and other enveloped viruses

Hodek

Zajícová

Lovětinská-Šlamborová

et al. 2016

BMC Microbiol

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BackgroundHealthcare-acquired infections by pathogenic microorganisms including viruses represent significant health concern worldwide. Next to direct transmission from person-to-person also indirect transmission from contaminated surfaces is well documented and important route of infections. Here, we tested antiviral properties of hybrid coating containing silver, copper and zinc cations that was previously shown to be effective against pathogenic bacteria including methicillin-resistant Staphylococcus aureus. Hybrid coatings containing silver, copper and zinc cations were prepared through radical polymerization via sol-gel method and applied on glass slides or into the wells of polymethylmethacrylate plates. A 10 μl droplet of several viruses such as human immunodeficiency virus type 1 (HIV-1), influenza, dengue virus, herpes simplex virus, and coxsackievirus was added to coated and uncoated slides or plates, incubated usually from 5 to 240 min and followed by titer determination of recovered virus.ResultsScanning electron microscopy analysis showed better adhesion of coatings on glass surfaces, which resulted in 99.5–100 % HIV-1 titer reduction (3.1 ± 0.8 log10TCID50, n = 3) already after 20 min of exposure to coatings, than on coated polymethylmethacrylate plates with 75–100 % (1.7 ± 1.1 log10TCID50, n = 3) and 98–100 % (2.3 ± 0.5 log10TCID50, n = 3) HIV-1 titer reduction after 20 and 120 min of exposure, respectively. Slower virucidal kinetics was observed with other enveloped viruses, where 240 min exposure to coated slides lead to 97 % (dengue), 100 % (herpes simplex) and 77 % (influenza) reduction in virus titers. Interestingly, only marginal reduction in viral titer after 240 min of exposure was noticed for non-enveloped coxsackie B3 virus.ConclusionsOur hybrid coatings showed virucidal activity against HIV and other enveloped viruses thus providing further findings towards development of broad-spectrum antimicrobial coating suitable for surfaces in healthcare settings.

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Section: Introductionmentioning

confidence: 99%

Protective hybrid coating containing silver, copper and zinc cations effective against human immunodeficiency virus and other enveloped viruses

Hodek

Zajícová

Lovětinská-Šlamborová

et al. 2016

BMC Microbiol

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“…As previously discussed, OrmoComp is particularly advantageous as a surface for cell adhesion and has vast potential as a polymer for artificial ECM scaffolds, which can be specifically designed to encourage cell migration and ultimately tissue formation . Figure displays the false‐colour–shaded 3D map of a Caco‐2 cell adhered and fixed to an OrmoComp‐fabricated grid.…”

Section: Resultsmentioning

confidence: 99%

“…These difficulties are being overcome through computer‐based microfabrication and nanofabrication of tailored 3D scaffolds (e.g., 3D printing set‐ups, laser‐based systems, and nozzle‐based systems) composed of various synthetic polymeric polymers, which have the advantage of producing scaffolds with controlled chemical composition, geometry, and mechanical properties . One particularly successful fabrication approach for 3D scaffolds suitable for cell culture has been the use of direct‐laser writing (DLW) . DLW provides a versatile approach enabling the fabrication of various sized scaffolds from a variety of polymers including mixtures of polydimethylsiloxane, poly(ethyleneglycol)‐diacrylate, pentaerythritol tetracrylate, and OrmoComp (a protein‐binding photoresist containing Irgacure as a photoinitiator).…”

Section: Introductionmentioning

confidence: 99%

“…DLW provides a versatile approach enabling the fabrication of various sized scaffolds from a variety of polymers including mixtures of polydimethylsiloxane, poly(ethyleneglycol)‐diacrylate, pentaerythritol tetracrylate, and OrmoComp (a protein‐binding photoresist containing Irgacure as a photoinitiator). In particular, OrmoComp has been extensively demonstrated to be a good polymer for cell adhesion . Greiner et al fabricated OrmoComp micro‐scale scaffolds, using the two‐photon polymerization DLW technique, and successfully observed variations in cell migration across the scaffold surface of cells depending on nuclear stiffness .…”

Section: Introductionmentioning

confidence: 99%

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Single‐cell Raman microscopy of microengineered cell scaffolds

Baldock

Talari

Smith

et al. 2018

J Raman Spectroscopy

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Studying cells in a three-dimensional (3D) environment has great potential in understanding cell behaviours such as morphology, proliferation, differentiation, and migration. Microengineered 3D cell scaffolds with precise defined geometries have offered a new approach to study cell behaviour and its interactions with scaffolds. The use of Raman spectroscopy to characterise biomolecules is a rapidly expanding area and has been implemented in numerous fields including pharmacology, microbiology, toxicology, and single-cell studies.However, one area where it remains unexploited despite the vast potential of the technique is in the investigation of 3D cell scaffolds. A combination of Raman microscopy and chemometric approaches have employed to investigate the structure and biochemistry of nanofabricated scaffolds and a cell-scaffold complex. The 3D Raman mapping combined with the use of nanofabricated 3D scaffolds offers a unique opportunity to assess the influence of scaffold architecture on cell body and cell nuclei morphology and biochemistry. For the first time, we have cultured a human epithelial colorectal adenocarcinoma cell line on OrmoComp scaffolds and determined the structure and biochemistry of nanofabricated scaffolds and a cell-scaffold complex with the use of Raman microscopy combined with appropriate data analysis protocols. The results demonstrate the potential of 3D Raman mapping for identifying biochemical and physical variation within single cells as they grow and adhere to 3D scaffolds.

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“…Hard stamps, such as silicon or inorganic-organic hybrid polymers, that is, Ormocers, are ideal candidates for T-NIL with squeeze flow in which a pressure is applied for a complete filling of the structures 42 , while soft stamps, such as UV-curable polydimethyl siloxane (UV-PDMS), enable conformation to surface undulations of the moulds by capillary action [43][44][45] . First, the fabrication of the two different masters is presented.…”

Section: Fabrication Approach Considerationsmentioning

confidence: 99%

High-aspect-ratio nanoimprint process chains

et al. 2017

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Different methods capable of developing complex structures and building elements with high-aspect-ratio nanostructures combined with microstructures, which are of interest in nanophotonics, are presented. As originals for subsequent replication steps, two families of masters were developed: (i) 3.2 μm deep, 180 nm wide trenches were fabricated by silicon cryo-etching and (ii) 9.8 μm high, 350 nm wide ridges were fabricated using 2-photon polymerization direct laser writing. Both emerging technologies enable the vertical smooth sidewalls needed for a successful imprint into thin layers of polymers with aspect ratios exceeding 15. Nanoridges with high aspect ratios of up to 28 and no residual layer were produced in Ormocers using the micromoulding into capillaries (MIMIC) process with subsequent ultraviolet-curing. This work presents and balances the different fabrication routes and the subsequent generation of working tools from masters with inverted tones and the combination of hard and soft materials. This provides these techniques with a proof of concept for their compatibility with high volume manufacturing of complex micro-and nanostructures.Keywords: cryo-etching; direct 6 write laser lithography; high aspect ratio; moulding; nanoimprint lithography; Ormocer; photonic nanofences; 2-photon polymerization INTRODUCTION High-aspect-ratio microstructures (HARMS), that is, structures with a large height/width ratio, have found many applications 1,2 such as X-ray gratings 3 , gas chromatography columns 4 , magnetic coils 5 , X-ray telescopes 6 , micro-gears 7 , micro-capacitors with highaspect-ratio (HAR) cantilevers 8 , and micromechanical and microoptical elements 9,10 . These structures exhibit aspect ratios (ARs) of over 10 and almost vertical sidewalls, making them much more challenging to develop than the structures typically used in planar technology with moderate ARs of 1 to 2. They are therefore characterized by an intermediate state between two-and threedimensional (2D and 3D), that is, a 2D planar design that is extended into the vertical dimension and often called . There are several methods to produce HAR structures on typical planar substrates, most of them relying on the anisotropy of subtractive pattern transfer of a low AR masking layer into an underlying resist or substrate, that is, by using mask based ultraviolet (UV)-photolithography (PL) with an AR up to 5, silicon etching (for example, dry etching) with an AR up to 20, and direct write laser lithography (DWL) and X-ray lithography with ARs 420 (Refs. 12-15). These methods are continually being improved to produce a higher AR and smaller lateral dimensions. Furthermore, they are enlarged by new techniques that are often subsets of etching processes with different advantages and disadvantages. One of those challenges is the suitability for submicron and nanopatterning, that is, structures with lateral sizes much smaller than 1 μm and the possibility to combine micro-with nanostructures in the same material.There are three main approaches: (1)...

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Mechanical characteristics of an Ormocomp® biocompatible hybrid photopolymer

Cited by 43 publications

References 27 publications

Protective hybrid coating containing silver, copper and zinc cations effective against human immunodeficiency virus and other enveloped viruses

Protective hybrid coating containing silver, copper and zinc cations effective against human immunodeficiency virus and other enveloped viruses

Single‐cell Raman microscopy of microengineered cell scaffolds

High-aspect-ratio nanoimprint process chains

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